Optimization and Technological Development Strategies of an Antimicrobial Extract from Achyrocline alata Assisted by Statistical Design

Demarque, Daniel P.; Fitts, Sonia Maria Fernandes; Boaretto, Amanda Galdi; Silva, Julio Cesar Leite da; Vieira, M.C.; Franco, Vanessa; Teixeira, Caroline B.; Toffoli-Kadri, Mônica Cristina; Carollo, Carlos Alexandre

doi:10.1371/journal.pone.0118574

Cited by 8 publications

(7 citation statements)

References 41 publications

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“…Nanotechnology approach can increase the stability and also improve the therapeutic efficiency of phytochemicals, such as polyphenols, tannins, alkaloids, terpenoids and herbal extracts. Since ancient times, compounds derived from plants have been a good source of therapeutic drugs [1]. In recent years, there has been growing interest in alternative therapies and therapeutic use of natural materials such as Taxol (a substance originated from the Pacific yew tree) and they have been approved for the clinical treatment of cancer patients [2].…”

Section: Introductionmentioning

confidence: 99%

Lawsone-loaded Niosome and its antitumor activity in MCF-7 breast Cancer cell line: a Nano-herbal treatment for Cancer

et al. 2018

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Phytochemicals like Lawsone have some drawbacks that stem from their poor solubility. Low solubility in aqueous mediums results in low bioavailability, poor permeability and instability of phytochemical compounds in biological environments. The aim of this study was to design nanoniosomes containing Lawsone (Law) using non-ionic surfactants and cholesterol. Niosomes were prepared by thin film hydration method (TFH). Then, they were loaded with Henna extract (HLaw) and standard Lawsone (SLaw), and two resulted formulations were compared. The henna extract was analyzed by mass gas chromatography. Size, zeta potential, polydispersity index (PDI) and morphology of the loaded formulations were evaluated by dynamic light scattering (DLS) and scanning electron spectroscopy (SEM). The incorporation and release rate of Law from niosome bilayers were evaluated by UV-Vis spectroscopy. In vitro experiments were carried out to evaluate antitumor activity in MCF-7 cell line. The results showed distinct spherical shapes and particle sizes were about 250 nm in diameter and have negative zeta potentials. Niosomes were stable at 4 °C for 2 months. Entrapment efficiently of both formulations was about 70% and showed a sustained release profile. In vitro study exhibited that using of niosome to encapsulating Law can significantly increase antitumor activity of formulation in MCF-7 cell line compared to Law solution (free Law). Thus, niosomes are a promising carrier system for delivery of phytochemical compounds that have poor solubility in biological fluids. Graphical abstract ᅟ.

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Section: Introductionmentioning

confidence: 99%

Lawsone-loaded Niosome and its antitumor activity in MCF-7 breast Cancer cell line: a Nano-herbal treatment for Cancer

et al. 2018

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“…tory actions (9)(10)(11)(12). Achyrocline species subjected to chemical and biological studies include A. satureioides (13)(14)(15)(16), A. alata (17)(18)(19), A. tomentosa, (20,21) and A. flaccida (22,23). The profile of their biological activities include anti-inflammatory, anti-infective and antioxidant effects related, at least in part, to their polyphenol components.…”

Section: Palabras Clavementioning

confidence: 99%

ALPHA ANTIADRENERGIC EFFECT OF Achyrocline bogotensis EXTRACT (“Vira Vira”) IN ISOLATED RAT AORTIC RING

Lara

Rincón

Guerrero

2017

Vitae

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Background: The treatment of symptoms of prostatic hyperplasia is among the traditional uses of Achyrocline bogotensis (Kunth) [N.V. "Vira Vira", Compositae] in Colombia. Pharmacological therapy for this disorder depends mainly on alpha-1 antiadrenergic agents, and the mechanism has not been studied previously using A. bogotensis. Objectives: To assess the alpha-1 antiadrenergic effect of the extract obtained from the aerial parts of A. bogotensis in isolated aortic rings from Wistar rats. Methods: The study compared the effects of the ethanol extract of A. bogotensis, prazosin (reference) and DMSO (control) in rings stimulated with phenylephrine (PE) or KCl. The capacity to reduce the PE pressor effect by the ethanol extract (pD 2 ' value) was determined. To quantify the A. bogotensis relaxant potency, increasing concentrations of the ethanol extract (0.1 µg/mL-0.1 mg/mL), were added cumulatively to isolated aortic rings pre-contracted with PE (0.1 µM) or KCl (80 mM). To explore the possible participation of nitric oxide (NO), L-NAME (100 µM) was administered to aortic rings exposed to cumulatively increasing concentrations of PE in isolated aortic rings in the presence of the extract (10 µg/mL). Aqueous, butanol and dichloromethane fractions (10 µg/mL) obtained from the ethanol extract were assayed. Phytochemical screening was also performed. Results: Prazosin and A. bogotensis extract notably reduced the contraction induced by PE whereas their inhibitory effect in rings contracted with KCl were lower. A. bogotensis ethanol extract showed a high capacity for reducing the PE pressor response (pD´2: 5.51) as well as total efficacy for relaxing rings previously precontracted with PE. The relaxant efficacy and potency of A. bogotensis extract against rings previously contracted with KCl were notably lower. L-NAME partly reverted the inhibitory effect of A. bogotensis. Aqueous, butanol and dichloromethane fractions gave inhibitory responses lower than that obtained with the ethanol extract. Phytochemical screening of A. bogotensis extract revealed the significant presence of flavonoid and triterpene metabolites. Conclusions: These results suggest that A. bogotensis elicits a smooth muscle relaxant effect related to the alpha-1 antiadrenergic mechanism. This response is partially NO dependent and seems to be due to interactions among active metabolites likely to be of flavonoid and/or terpenoid nature.

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“…Reducing the costs and making the processes economically viable by optimizing the operational key parameters is the ultimate objective of basic research for industrial applications (Asgher, Yasmeen, & Iqbal, 2014). Response Surface Methodology (RSM) is a statistical approach that widely used for developing, improving, and optimizing processes (Demarque et al, 2015;Hayes et al, 2005), and it determinates more than one factors at a time at different levels providing optimization results in a true sense (Rengadurai, Preetha, & Viruthagiri, 2012). Recently, it has been successfully applied in other scientific fields such as biology, medicine, and economy for optimization purposes (Curiel et al, 2004;Demarque et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Response Surface Methodology (RSM) is a statistical approach that widely used for developing, improving, and optimizing processes (Demarque et al, 2015;Hayes et al, 2005), and it determinates more than one factors at a time at different levels providing optimization results in a true sense (Rengadurai, Preetha, & Viruthagiri, 2012). Recently, it has been successfully applied in other scientific fields such as biology, medicine, and economy for optimization purposes (Curiel et al, 2004;Demarque et al, 2015). The objective of the current study was to evaluate the in-vitro antibacterial activity of methanolic extract of leaves of Eucalyptus tereticornis using RSM.…”

Section: Introductionmentioning

confidence: 99%

Optimization of antibacterial activity of Eucalyptus tereticornis leaf extracts against Escherichia coli through response surface methodology

Ammer

Zaman

Khalid

et al. 2016

Journal of Radiation Research and Applied Sciences

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Medicinal plants are the important source of potentially useful chemotherapeutic agents which have made enormous contributions to human health and well-being. The methanolic extract of leaves of Eucalyptus tereticornis for antibacterial activity against the bacterial strain Escherichia coli was investigated. Response surface methodology (RSM) using central composite design (CCD) was adopted to optimize the effect of process variables.The optimum conditions for maximum antibacterial activity (zones of inhibition, mm) were found out to be E. tereticornis extract i.e., Limonene, 3 mL; 1, 8-Cineole, 11 mL; Terpinen-4-ol, 15 mL; pH, 7.0 and temperature, 40 C. The results suggest that the organic (methanol) extract of E. tereticornis could be a possible source to obtain new and effective herbal medicines to combat infections caused by multi-drug resistant microbial strains from community and hospital settings.

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Optimization and Technological Development Strategies of an Antimicrobial Extract from Achyrocline alata Assisted by Statistical Design

Cited by 8 publications

References 41 publications

Lawsone-loaded Niosome and its antitumor activity in MCF-7 breast Cancer cell line: a Nano-herbal treatment for Cancer

Lawsone-loaded Niosome and its antitumor activity in MCF-7 breast Cancer cell line: a Nano-herbal treatment for Cancer

ALPHA ANTIADRENERGIC EFFECT OF Achyrocline bogotensis EXTRACT (“Vira Vira”) IN ISOLATED RAT AORTIC RING

Optimization of antibacterial activity of Eucalyptus tereticornis leaf extracts against Escherichia coli through response surface methodology

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