Optimization and scale-up of propionic acid production by propionic acid-tolerant Propionibacterium acidipropionici with glycerol as the carbon source

Zhu, Yunfeng; Li, Jianghua; Tan, Teck Ming; Liu, Long; Jiang, Lili; Sun, Jun; Lee, Pengsoon; Du, Guocheng; Chen, Jian

doi:10.1016/j.biortech.2010.06.070

Cited by 100 publications

(68 citation statements)

References 21 publications

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“…While propionate biosynthesis has primarily been conducted by anaerobic cultivation of Gram-positive native producers, such as Propionibacteria and Clostridia [2,15,43,45], or by anaerobic E. coli cultivation but with limited propionate titers [6,20], we herein present a novel approach through activation of the endogenous Sbm operon of E. coli. Further strain engineering resulted in high-level propionate production, with titers comparable to those achieved by anaerobic batch cultivation of Propionibacteria [2,8,15,30].…”

Section: Discussionmentioning

confidence: 99%

“…High-level production of propionate in these microorganisms, including P. ferudenreichii ssp shermanii, P. acidipropionici, and C. propionicum, using glucose and glycerol as carbon sources has been demonstrated with high yields [2,15,20,43,45]. Despite these successes, production platforms on the basis of these Gram-positive anaerobes are not ideal for various reasons, including their slow growth rate, the use of costly and complex media, and the lack of available genetic tools for strain engineering.…”

Section: Introductionmentioning

confidence: 97%

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Engineering Escherichia coli for high-level production of propionate

Akawi

Srirangan

Liu

et al. 2015

Journal of Industrial Microbiology and Biotechnology

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Mounting environmental concerns associated with the use of petroleum-based chemical manufacturing practices has generated significant interest in the development of biological alternatives for the production of propionate. However, biological platforms for propionate production have been limited to strict anaerobes, such as Propionibacteria and select Clostridia. In this work, we demonstrated high-level heterologous production of propionate under microaerobic conditions in engineered Escherichia coli. Activation of the native Sleeping beauty mutase (Sbm) operon not only transformed E. coli to be propionogenic (i.e., propionate-producing) but also introduced an intracellular "flux competition" between the traditional C2-fermentative pathway and the novel C3-fermentative pathway. Dissimilation of the major carbon source of glycerol was identified to critically affect such "flux competition" and, therefore, propionate synthesis. As a result, the propionogenic E. coli was further engineered by inactivation or overexpression of various genes involved in the glycerol dissimilation pathways and their individual genetic effects on propionate production were investigated. Generally, knocking out genes involved in glycerol dissimilation (except glpA) can minimize levels of solventogenesis and shift more dissimilated carbon flux toward the C3-fermentative pathway. For optimal propionate production with high C3:C2-fermentative product ratios, glycerol dissimilation should be channeled through the respiratory pathway and, upon suppressed solventogenesis with minimal production of highly reduced alcohols, the alternative NADH-consuming route associated with propionate synthesis can be critical for more flexible redox balancing. With the implementation of various biochemical and genetic strategies, high propionate titers of more than 11 g/L with high yields up to 0.4 g-propionate/g-glycerol (accounting for ~50 % of dissimilated glycerol) were achieved, demonstrating the potential for industrial application. To our knowledge, this represents the most effective engineered microbial system for propionate production with titers and yields comparable to those achieved by anaerobic batch cultivation of various native propionate-producing strains of Propionibacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Engineering Escherichia coli for high-level production of propionate

Akawi

Srirangan

Liu

et al. 2015

Journal of Industrial Microbiology and Biotechnology

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“…The microorganisms in this process are Propionibacterium (Himmi et al, 2000;A. Zhang & Yang, 2009;Zhu et al, 2010). Propionic acid is widely used in chemical, pharmaceutical and food industries.…”

Section: Propionic Acidmentioning

confidence: 99%

“…shermanii strain was used in comparison to cultivation feeding by glucose. Zhu et al (2010) converted glycerol to propionic acid using propionic acid-tolerant strain of Propionibacterium acidipropionici in a large scale of 10m 3 bioreactor. The highest propionic acid production reached ca.…”

Section: Propionic Acidmentioning

confidence: 99%

Improved Utilization of Crude Glycerol By-Product from Biodiesel Production

Kośmider¹,

Leja²,

Czaczyk³

2011

Biodiesel- Quality, Emissions and by-Products

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“…[17] Propionibacterium are able to use different carbon sources such as glucose, [18,19] maltose, [20] sucrose, [21] lactose, [22,23] lactate [19,24] and glycerol. [25,26] It can also use complex sources such as hemicelluloses, corncob molasses and sugarcane molasses and etc. [27] Since, the application of conventional and expensive systems of fermentation is limited due to the low concentration, yield and productivity, the increased yields of propionic acid obtained by fermentation of cheap industrial waste e.g.…”

Section: Introductionmentioning

confidence: 99%

Fed-Batch Fermentation for Propionic, Acetic and Lactic Acid Production

Ahmadi¹,

Zarean-Shahraki²,

Mortazavian³

et al. 2015

Orient. J. Chem

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Propionibacterium is capable of producing many important industrial products such as propionic acid, vitamin B 12 and bacteriocin. Also it shows some probiotic health benefits and produces stimulator of intestinal bacteria. The aim of this research is to evaluate the effect of pH control on biomass, propionic, acetic and lactic acids production in fed-batch system by Propionibacterium freudenreichii ssp. shermanii and Lactobacillus acidophilus. Fermentation was conducted in a 3-L fermentor containing base medium and molasses as the carbon source in which milk feeding were added in 36th hour and maintained at 30 °C. Total fermentation time was 144 h. Every 24 h sampling has been done to measure the biomass and organic acids. Yield of biomass and acid production were compared in two separate trials: one treatment without pH control and another in which pH was maintained at 6.5 by using NaOH 1N. Content of biomass and organic acid were measured by freeze drying method and HPLC, respectively. The final concentration of the obtained responses in treatments with and without pH control is as following (g/L): biomass 6.22±0.04 and 13.76 ±0.04; propionic acid, 5.25±0.02 and 5.67±0.01; acetic acid 5.86±0.02 and 6.33±0.06; and lactic acid 11.34±0.09 and 7.40±0.07. In treatment which pH has not been controlled, production of dried biomass, propionic and acetic acid were higher than controlled treatment. So production of a beverage containing propionic acid could be recommended due to satiety-inducing and saturation effect in consumer.

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Optimization and scale-up of propionic acid production by propionic acid-tolerant Propionibacterium acidipropionici with glycerol as the carbon source

Cited by 100 publications

References 21 publications

Engineering Escherichia coli for high-level production of propionate

Engineering Escherichia coli for high-level production of propionate

Improved Utilization of Crude Glycerol By-Product from Biodiesel Production

Fed-Batch Fermentation for Propionic, Acetic and Lactic Acid Production

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