Optimization and partial purification of a protease produced by selected bacterial strains grown on trash fish meal substrate and its antagonistic property against bacterial pathogens

Prakash, S.; Ramasubburayan, Ramasamy; Iyapparaj, Palanisamy; Sankaralingam, S.; Palavesam, A.; Immanuel, G.

doi:10.1016/j.bcab.2014.09.006

“…Carbon source is important for the growth of all microbes, but the ability to utilize a particular carbon source may vary from species to species. However, several authors suggest that between both carbon sources, maltose was most preferred than sucrose (Yang et al 2000;Ellaiah et al 2002;Prakash et al 2014). In these studies, maltose was most efficiently utilized by the microorganism than sucrose, which resulted in optimum protease production.…”

Section: Effects Of Media Components and Physical Factors On Protease...mentioning

confidence: 97%

Optimization and Functional Properties of a Protease from a Fish Gut Isolate Pseudomonas sp. PD14 Grown on Fish Processing Wastes Substrate

Armada¹,

Simora²

2021

Philipp J Sci

0

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Extracellular protease production from Pseudomonas sp. PD14 isolated from the gut of rabbitfish, Siganus guttatus, was investigated using fish processing wastes as nitrogen source. Three types of fish processing by-products – namely, heads, bones, and viscera – from yellowstripe scad (Selaroides sp.) and Japanese halfbeak (Hyporhampus sajori), as well as trimmings and viscera from squid (Loligo sp.) were used. Among the fish waste preparations tested, Japanese halfbeak viscera (HV) flour (1.0%) supported the maximum protease production of 365.0 U/mg protein. Effects of media components on protease production in the optimized concentration of HV flour revealed that the protease enzyme can be optimally produced using maltose (0.50%) as a carbon source, at pH 8.0, and incubated for 48 h at 40 °C. Partial characterization of the crude enzyme supernatant showed that the optimum pH and temperature were 8.0 and 50 °C, respectively. Furthermore, the crude protease exhibited thermostability retaining 50% activity at 60 °C and fairly stable up to pH 11.0. Considering the high costs of industrial enzyme production, the use of fish processing wastes for protease production offers a promising future for enzyme biotechnology.

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“…According to Rosenberg (Rosenberg et al, 2007) the marine environment includes a mega diversity of microorganisms that constitute a complex and intricate system of these ecological relations with the other biota elements and represent an important source of biomolecules, but it's still poorly explored comparing to others habitats, and their microorganisms are still to be described. As a consequence, natural microbial products are promising sources for the bioprospecting of new molecules with potential applications in medicine (pharmaceuticals), agriculture (agrochemicals) and in biological processes (chemical biology) studies (Prakash et al, 2014).…”

Section: Alterações Genéticas Somáticas No Osteossarcomamentioning

confidence: 99%

CITOGENOTOXICIDADE E MUTAGENICIDADE DO SULFATO DE COBRE EM DIFERENTES VARIEDADES DE allium cepa LINN

Messias¹,

Brito²,

Beltrão³

et al. 2019

Conceitos Básicos Da Genética

2

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“…According to Rosenberg (Rosenberg et al, 2007) the marine environment includes a mega diversity of microorganisms that constitute a complex and intricate system of these ecological relations with the other biota elements and represent an important source of biomolecules, but it's still poorly explored comparing to others habitats, and their microorganisms are still to be described. As a consequence, natural microbial products are promising sources for the bioprospecting of new molecules with potential applications in medicine (pharmaceuticals), agriculture (agrochemicals) and in biological processes (chemical biology) studies (Prakash et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Dna Barcoding Confirma a Ocorrência De Espécies Amazônicas Na Ictiofauna Do Rio Turiaçu, Maranhão/Brasil

Teixeira¹,

Barros²,

Fraga³

2019

Conceitos Básicos Da Genética

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Optimization and partial purification of a protease produced by selected bacterial strains grown on trash fish meal substrate and its antagonistic property against bacterial pathogens

Cited by 11 publications

References 36 publications

Optimization and Functional Properties of a Protease from a Fish Gut Isolate Pseudomonas sp. PD14 Grown on Fish Processing Wastes Substrate

Optimization and Functional Properties of a Protease from a Fish Gut Isolate Pseudomonas sp. PD14 Grown on Fish Processing Wastes Substrate

CITOGENOTOXICIDADE E MUTAGENICIDADE DO SULFATO DE COBRE EM DIFERENTES VARIEDADES DE allium cepa LINN

Dna Barcoding Confirma a Ocorrência De Espécies Amazônicas Na Ictiofauna Do Rio Turiaçu, Maranhão/Brasil

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