Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots

Mudrić, Jelena; Janković, Teodora; Šavikin, Katarina; Bigović, Dubravka; Đukić-Ćosić, Danijela; Ibrić, Svetlana; Đuriš, Jelena

doi:10.1016/j.indcrop.2020.112767

Cited by 23 publications

(16 citation statements)

References 40 publications

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“…The insignificant effect of HP β CD concentration can be attributed to the good water solubility of gentiopicroside itself, thus making dissolution support redundant. The prominent effect of extraction temperature is consistent with previous studies in which UAE optimization of G. lutea root was performed [ 27 , 36 ], where the maximum amount of gentiopicroside content was obtained using the highest tested temperatures (65 and 80 °C, respectively). Also, Ariño et al [ 37 ] demonstrated that the content of this secoiridoid compound in yellow gentian root extracts continuously rose with the increase of temperature up to 75 °C.…”

Section: Resultssupporting

confidence: 90%

“…The minimal isogentisin content was obtained after an application extraction temperature of 20 °C, an extraction time of 35 min, and a HP β CD concentration of 2% ( w / v ). In our earlier works, mainly higher contents of isogentisin were obtained from the underground parts of G. lutea and G. asclepiadea , but after using water-ethanol as the solvent [ 26 , 27 ]. On the other hand, the conditions that we defined in this work for the maximal water extraction of isogentisin from willow gentian “dust” allowed for a larger amount of this compound than after using 10% of ethanol (minimum tested concentration) in our previous study on willow gentian underground parts (0.46 mg/g DW) [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies show that one of the main strategies to improve the extraction of isogentisin is the use of high concentrations of ethanol in a water-ethanol solvent [ 26 , 27 , 36 ]. Consequently, the use of HP β CD as a co-solvent in a water solvent may be a valuable alternative strategy to improve the extraction of isogentisin if it is necessary to avoid the utilization of organic solvents.…”

Section: Resultsmentioning

confidence: 99%

“…The traditional “one-factor-at-a-time” optimization approach is time- and material-consuming and inefficient for evaluating the interaction effect of process parameters. These limitations can be overcome by employing statistical and mathematical tools such as the response surface methodology (RSM) [ 27 ]. The Box–Behnken design (BBD) and central composite design (CCD) are the most commonly used response surface designs for the optimization of the extraction process.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules

et al. 2022

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The residue after sieving (“dust”) from the willow gentian underground parts is an unexploited herbal tea by-product, although it contains valuable bioactive compounds. Cyclodextrins as efficient green co-solvents, cage molecules, and multifunctional excipients could improve the extraction and contribute to the added value of the resulting extracts. The objective of this study was to determine the optimal conditions for the extraction of gentiopicroside, isogentisin, and total phenolics (TPC) from willow gentian “dust” using ultrasound-assisted water extraction coupled with hydroxypropyl-β-cyclodextrin (HPβCD). The influence of extraction temperature (X1: 20–80 °C), time (X2: 20–50 min), and HPβCD concentration (X3: 2–4% w/v) was analyzed employing the response surface methodology (RSM). The optimal extraction conditions for simultaneously maximizing the extraction yield of all monitored responses were X1: 74.89 °C, X2: 32.57 min, and X3: 3.01% w/v. The experimentally obtained response values under these conditions (46.96 mg/g DW for gentiopicroside, 0.51 mg/g DW for isogentisin, and 12.99 mg GAE/g DW for TPC) were in close agreement with those predicted, thus confirming the suitability and good predictive accuracy of the developed RSM models. Overall, the developed extraction system could be an applicable alternative strategy to improve the extraction of bioactive compounds from the underutilized “dust” of willow gentian underground parts.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules

et al. 2022

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“…Plant material, Gentiana lutea roots, was purchased from the Institute for Medicinal Plants Research "Dr. Josif Pančić" (Belgrade, Serbia). Liquid gentian root extract was obtained by heat-assisted extraction under the optimal conditions described by Mudrić et al (2020). Extraction in a thermostated water bath (Grant, model LSB 18, United Kingdom) lasted 129 minutes, at a temperature of 65°C, ethanol concentration was 49 % and the solid-to-solvent ratio was 1:40.…”

Section: Preparation Of Dry Extractmentioning

confidence: 99%

Development of gastroretentive floating granules with gentian root extract by hot-melt granulation

Mudrić¹,

Pajić²,

Bigović³

et al. 2020

Lekovite sirovine

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The roots of yellow gentian, Gentiana lutea L. (Gentianaceae) are used in traditional medicine for the treatment of gastrointestinal disorders, with the literature data indicates a local gastric effect of gentian root extract (GRE) and support the use of the solid pharmaceutical forms. Gentiopicroside, as a dominant secoiridoid in the GRE, has a short elimination half-life and low bioavailability and, consequently, its bioactivity is limited. The aim of the study was to develop gastroretentive floating delivery system with GRE, and to provide prolonged release of gentiopicroside. Floating granules with dry GRE (DGRE) were prepared by the hot-melt granulation technique, while formulations included effervescent components (citric acid and sodium bicarbonate), hydroxypropyl methylcellulose (HPMC) and meltable binders (Compritol® 888 ATO and Gelucire® 50/13). The flowability of the DGRE and prepared formulations was determined by calculating the Carr index and Hausner ratio. Floating properties and in vitro dissolution rate of gentiopicroside from investigated formulations were examined. Floating granules were characterized with improved flowability (Carr index 14-22 %; Hausner ratio 1.16-1.28) in comparison to the DGRE (Carr index 28.99 %; Hausner ratio of 1.41). Furthermore, the floating granules exhibited immediate and long-lasting buoyancy and prolonged-release of gentiopicroside (over 8 h). Compritol® 888 ATO has provided sustained release of gentiopicroside from floating granules, while HPMC has decreased release rate additionally. On the other hand, Gelucire® 50/13 has increased gentiopicroside release rate. The results have shown that hot-melt granulation technique, as a green granulation method was successfully employed for obtaining gastroretentive floating granules with DGRE.

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Unlocking hot trub's potential: a simple method for extracting bitter acids and xanthohumol

Silva,

Feltre,

Zandonadi

et al. 2024

J Sci Food Agric

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BackgroundHot trub is a macro‐ and micronutrient‐rich by‐product generated in the brewing industry, which is still underrated as a raw material for reprocessing purposes. In this context, this study aimed to investigate the extraction of bitter acids' and xanthohumol from hot trub as well as identify the significance of parameters for the process. The research assessed various extraction parameters, such as pH, ethanol concentration, temperature, and solid‐to‐liquid ratio, using a Plackett‐Burman design.ResultsEthanol concentration and pH were the most significant parameters affecting extraction yield. β‐acids were found to be the principal components of the bitter acids, with a maximum concentration near 16 mg/g, followed by iso‐α‐acids and α‐acids achieving 6 and 3.6 mg/g, respectively. The highest yields of bitter acids were observed in the highest ethanol concentration, while pH was relevant to extraction process in treatments with low ethanol ratios. Concerning the xanthohumol extraction, the approach achieved maximum concentration (239 μg/g) in treatments with ethanol concentration above 30%. Despite their variances, the phytochemicals exhibited comparable extraction patterns, indicating similar interactions with macromolecules. Moreover, the solid residues' characterization demonstrated that the extraction process did not bring about any alterations to the chemical and total protein profiles.ConclusionEthanol concentration was found to have the most significant impact on the extraction of bitter acids and xanthohumol, while temperature had no significant effect. The solid remains resulting from the extraction showed potential for use as a protein source.This article is protected by copyright. All rights reserved.

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Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots

Cited by 23 publications

References 40 publications

Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules

Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules

Development of gastroretentive floating granules with gentian root extract by hot-melt granulation

Unlocking hot trub's potential: a simple method for extracting bitter acids and xanthohumol

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