Optimization and kinetic studies for enzymatic hydrolysis and fermentation of colocynthis vulgaris Shrad seeds shell for bioethanol production

Nnaemeka, Igwilo Christopher; O, Egbuna Samuel; Maxwell, Onoh Ikechukwu; Christain, Asadu O.; S, Onyekwulu Chinelo

doi:10.1016/j.jobab.2021.02.004

Cited by 39 publications

(9 citation statements)

References 21 publications

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“…The optimization of physico-chemical factors is a key step in the success of enzymatic processes, as defining the optimum of each variable allows increased performance and better production [30].…”

Section: Discussionmentioning

confidence: 99%

Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions

et al. 2022

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Cellulose represents the most abundant component of plant biomass on earth; it is degraded by cellulases, specific enzymes produced by microorganisms. However, cellulases of bacterial origin attract more interest due to their natural diversity and ability to inhabit a variety of niches, allowing the selection of cellulolytic strains resistant to environmental stresses. The screening of the cellulolytic activity of 398 bacteria isolated from various ecosystems in Algeria (cave, ruins, chott, thermal station, and rhizosphere of arid and semi-arid regions) was performed by the appearance of a hydrolysis zone on carboxymethylcellulose (CMC) medium. The cellulase activity on CMC (1%) broth allowed to select 26 strains among which 12 had the best activity (0.3 U/mL to 2.2 U/mL). Optimization of physicochemical parameters (salinity: 0–1 M NaCl; pH: 3, 4, 7, 9, and 11; temperature: 30, 45, and 50 °C; PEG8000: 0 and 30%) involved in growth and cellulose production showed that the majority of strains were mesophilic, neutrophilic, or alkali- tolerant and tolerant to 30% of PEG8000. The cellulase activity and stability under different stress allowed to retain five strains, which the most efficient. Based on the 16S-rRNA sequencing results, they belonged to the genus Bacillus. The physicochemical properties of cellulases (crude extract) showed a CMCase active over a wide range of pH (4 to 11), optimal at 50 °C and 60 °C. The inhibiting salinity effect on the activity was not detected and was negligible on the enzymatic stability. The residual CMCase activity remained between 40 and 70% in a temperature range between 40 and 70 °C, was stable over a wide range of saline concentrations (0–2000 mM), and was weakly affected at 30% of PEG8000. The crude enzyme extract was able to hydrolyze both soluble and insoluble cellulosic substrates. The evaluation of the hydrolysis capacity of lignocellulosic waste revealed the ability of tested strains to degrade wheat bran, barley bran, and corncob. In addition, the enzyme showed significant multi-stress resistance on solid and liquid media. By these characteristics, these cellulolytic bacteria could be attractive to be used in various industrial and biotechnology applications.

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Section: Discussionmentioning

confidence: 99%

Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions

et al. 2022

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“…"Optimization is concerned with selecting the best among the entire set by efficient quantitative methods. The goal of optimization however, is to find the values of the variables in the process to yield the best value of the performance criterion" [12]. The filtration loss was therefore, optimized with the design expert to minimize the volume of fluid loss.Maximum filtration loss of 11.58ml was obtained at optimum conditions of concentration of additive (GNC), 6g and filtration time of 15minutes.…”

Section: Validation Of the Optimum Parameters Of The Mudmentioning

confidence: 99%

“…None of the works in the aforementioned articles reported using the RSM tool to optimize the process variables. Igwilo et al, [12] claim that the use of software for data analysis as well as, the optimization of process parameters for optimal results are the current trends in research.…”

Section: Introductionmentioning

confidence: 99%

“…The main benefit is its ability to reduce the numerical strength of experiments, which is required to access the effects of many parameters as well as their interactions on the result. The improvement of industrial process characteristics accelerates industrial development, which eventually leads to profit [12]. RSM consequently focused on enhancing chemical processes to produce better results, such as higher yield and purity at lower costs.…”

Section: Introductionmentioning

confidence: 99%

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Optimization Study on the Filtration Loss of Water Based Drilling Fluid using Groundnut (Arachis hypogaea) Shells Cellulose

Maxwell

Chijioke²,

Onyejiuwa³

2022

JERR

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The key process parameters for the filtration loss of water-based drilling fluid (WBDF), using Arachishypogaea shells cellulose (AHSC) were optimized using the two-level-two factor full factorial design of Response Surface Methodology (RSM). This research reveals the key outcomes of investigations, carried out on the formulation of a sustainable drilling fluid system, where AHSC is used as a fluid loss additive having no toxicity and high biodegradability. The characterization and pre-treatment of the AHSC used were carried out. Meanwhile, the result of the proximate analysis revealed that the AHSC had 70.40% cellulose, which is a good additive for drilling mud formulation that displays related functions as some of the foreign fluid loss polymers like polyanionic cellulose used in producing drilling muds. It was established that, additive concentration and filtration time had significant effects on WBDF formulation and performance while the optimum results were obtained as: 6g, 15 minutes and 11.58ml for additive concentration, filtration time and filtration loss, respectively. This investigation showed the suitability of AHSC as fluid loss additive that is cost-effective and eco-friendly alternative in this challenging phase of the hydrocarbon exploration industry.

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“…However, it has proven difficult to develop nanomaterials with flexible properties that are desirable for meeting the growing demand for solutions to environmental problems worldwide. Only clean and cost-effective energy generation/storage technologies will allow us to achieve this goal [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Boron carbon nitride nanosheets in water and wastewater treatment: A critical review

et al. 2022

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Optimization and kinetic studies for enzymatic hydrolysis and fermentation of colocynthis vulgaris Shrad seeds shell for bioethanol production

Cited by 39 publications

References 21 publications

Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions

Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions

Optimization Study on the Filtration Loss of Water Based Drilling Fluid using Groundnut (Arachis hypogaea) Shells Cellulose

Boron carbon nitride nanosheets in water and wastewater treatment: A critical review

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