Optimization and Evaluation of the 30S-S11 rRNA Gene for Taxonomic Profiling of Oral Streptococci

O’Connell, Lauren M.; Blouin, Thomas; Soule, Ashlyn; Burne, Robert A.; Nascimento, Marcelle M.; Richards, Vincent P.

doi:10.1128/aem.00453-22

Cited by 8 publications

(7 citation statements)

References 69 publications

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“…In the current study, we could not monitor S. mutans using a partial region of the 16S rRNA gene. Moreover, Streptococcus species have poor taxonomic resolution below the genus level 50 . Future studies using shotgun whole genome sequencing are warranted for monitoring functional microbiome changes at species-level with higher resolution and accuracy as well as microbial association network analyses to identify interspecies interactions.…”

Section: Resultsmentioning

confidence: 99%

Iron oxide nanozymes stabilize stannous fluoride for targeted biofilm killing and synergistic oral disease prevention

Huang,

Liu,

Pandey

et al. 2023

Nat Commun

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Dental caries is the most common human disease caused by oral biofilms despite the widespread use of fluoride as the primary anticaries agent. Recently, an FDA-approved iron oxide nanoparticle (ferumoxytol, Fer) has shown to kill and degrade caries-causing biofilms through catalytic activation of hydrogen peroxide. However, Fer cannot interfere with enamel acid demineralization. Here, we show notable synergy when Fer is combined with stannous fluoride (SnF2), markedly inhibiting both biofilm accumulation and enamel damage more effectively than either alone. Unexpectedly, we discover that the stability of SnF2 is enhanced when mixed with Fer in aqueous solutions while increasing catalytic activity of Fer without any additives. Notably, Fer in combination with SnF2 is exceptionally effective in controlling dental caries in vivo, even at four times lower concentrations, without adverse effects on host tissues or oral microbiome. Our results reveal a potent therapeutic synergism using approved agents while providing facile SnF2 stabilization, to prevent a widespread oral disease with reduced fluoride exposure.

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Section: Resultsmentioning

confidence: 99%

Iron oxide nanozymes stabilize stannous fluoride for targeted biofilm killing and synergistic oral disease prevention

Huang,

Liu,

Pandey

et al. 2023

Nat Commun

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“…The distribution of ASVs belonging to a single species tends to be variable across health groups, with some ASVs increasing or decreasing along the six progressive disease states, a pattern recently documented among Streptococcus spp. ( 28 ). For example, while the relative abundance of Lachnospiraceae bacterium oral taxon 096 ASV1 and ASV1578 is relatively equivalent across samples from all six tooth health groups, ASV1575 is significantly higher in the H-CF than the H-CE group (Wilcoxon signed-rank test, false discovery rate [FDR] = 0.009) and significantly higher in H-CE compared to D-CD (Wilcoxon signed-rank test, FDR = 0.01).…”

Section: Resultsmentioning

confidence: 99%

Impact of HIV on the Oral Microbiome of Children Living in Sub-Saharan Africa, Determined by Using an rpoC Gene Fragment Metataxonomic Approach

et al. 2023

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Since its recognition as a global epidemic in the early 1980s, approximately 84.2 million people have been diagnosed with HIV and 40.1 million people have died from AIDS-related illnesses. The development and increased global availability of antiretroviral treatment (ART) regimens have dramatically reduced the mortality rate of HIV and AIDS, yet approximately 1.5 million new infections were reported in 2021, 51% of which are in sub-Saharan Africa.

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“…Furthermore, oral bacteria often co-aggregate, which can form microenvironments for competition/cooperation and may cause situational population dynamics to occur ( 55 ). Coupled with the difficulty in resolving Streptococcus species through 16S rRNA gene sequencing ( 56 ), we were unable to fully determine the relationships between the three S . mitis taxa and suggest that future research investigate the competition/cooperation of these bacteria, especially in the context of oral and systemic health.…”

Section: Discussionmentioning

confidence: 99%

Oral microbial communities in children, caregivers, and associations with salivary biomeasures and environmental tobacco smoke exposure

et al. 2023

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Human oral microbial communities are diverse, with implications for oral and systemic health. Oral microbial communities change over time; thus, it is important to understand how healthy versus dysbiotic oral microbiomes differ, especially within and between families. There is also a need to understand how the oral microbiome composition is changed within an individual including by factors such as environmental tobacco smoke (ETS) exposure, metabolic regulation, inflammation, and antioxidant potential. Using archived saliva samples collected from caregivers and children during a 90-month follow-up assessment in a longitudinal study of child development in the context of rural poverty, we used 16S rRNA gene sequencing to determine the salivary microbiome. A total of 724 saliva samples were available, 448 of which were from caregiver/child dyads, an additional 70 from children and 206 from adults. We compared children’s and caregivers’ oral microbiomes, performed “stomatotype” analyses, and examined microbial relations with concentrations of salivary markers associated with ETS exposure, metabolic regulation, inflammation, and antioxidant potential (i.e., salivary cotinine, adiponectin, C-reactive protein, and uric acid) assayed from the same biospecimens. Our results indicate that children and caregivers share much of their oral microbiome diversity, but there are distinct differences. Microbiomes from intrafamily individuals are more similar than microbiomes from nonfamily individuals, with child/caregiver dyad explaining 52% of overall microbial variation. Notably, children harbor fewer potential pathogens than caregivers, and participants’ microbiomes clustered into two groups, with major differences being driven by Streptococcus spp. Differences in salivary microbiome composition associated with ETS exposure, and taxa associated with salivary analytes representing potential associations between antioxidant potential, metabolic regulation, and the oral microbiome. IMPORTANCE The human oral cavity is a multi-environment habitat that harbors a diversity of microorganisms. This oral microbiome is often transmitted between cohabitating individuals, which may associate oral and systemic health within family members. Furthermore, family social ecology plays a significant role in childhood development, which may be associated with lifelong health outcomes. In this study, we collected saliva from children and their caregivers and used 16S rRNA gene sequencing to characterize their oral microbiomes. We also analyzed salivary biomeasures of environmental tobacco smoke exposure, metabolic regulation, inflammation, and antioxidant potential. We show there are differences in individuals’ oral microbiomes mainly due to Streptococcus spp. that family members share much of their microbes, and several bacterial taxa associate with the selected salivary biomeasures. Our results suggest there are large-scale oral microbiome patterns, and there are likely relationships between oral microbiomes and the social ecology of families.

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Optimization and Evaluation of the 30S-S11 rRNA Gene for Taxonomic Profiling of Oral Streptococci

Cited by 8 publications

References 69 publications

Iron oxide nanozymes stabilize stannous fluoride for targeted biofilm killing and synergistic oral disease prevention

Iron oxide nanozymes stabilize stannous fluoride for targeted biofilm killing and synergistic oral disease prevention

Impact of HIV on the Oral Microbiome of Children Living in Sub-Saharan Africa, Determined by Using an rpoC Gene Fragment Metataxonomic Approach

Oral microbial communities in children, caregivers, and associations with salivary biomeasures and environmental tobacco smoke exposure

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