Optimising plasmapheresis procedure: The role of plasma unit weight setting

Chiara, María Luisa Dalla; Merolle, Lucia; Rossi, Paolo Giorgi; Baricchi, Roberto; Pertinhez, Thelma A.

doi:10.1016/j.transci.2020.102963

Cited by 1 publication

(5 citation statements)

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“…The study required at least 54 donors (providing two donations each) to achieve a power of 80% and a level of significance of 5% (two-sided), to detect a mean of the differences of 4.3 mL in reinfused ACD-A between pairs, assuming the standard deviation of the differences to be 11 mL. The difference of 5 mL in reinfused blood was that observed in our observational study [14]. Similarly, we estimated the standard deviation from the mean according to the observed standard deviation of total ACD-A used [14].…”

Section: Methodsmentioning

confidence: 98%

“…The difference of 5 mL in reinfused blood was that observed in our observational study [14]. Similarly, we estimated the standard deviation from the mean according to the observed standard deviation of total ACD-A used [14]. During the analyses, according to the advice received by an external reviewer, we decided to apply a multiple test adjusted significance threshold.…”

Section: Methodsmentioning

confidence: 99%

“…Plasma collection was performed at the selected center (Casa del Dono di Reggio Emilia, Reggio Emilia, Italy), in accordance with the Italian Ministerial Decree of November 2, 2015 [3] , using the Plasma Collection System (PCS2, Haemonetics Corp., Rosemont, IL, USA), following the manufacturer’s instructions [16] . The PCS2 machine and the anticoagulant used (ACD-A; Haemonetics Corp., Rosemont, IL, USA) were the same as in our previously published communication [14] . All donors were recruited between February 2022 and March 2021 ( Fig.…”

Section: Methodsmentioning

confidence: 99%

“…In a recent paper comparing two blood donor centers, with apparently identical plasmapheresis protocols and machines (PCS2; Haemonetics Corp., Rosemont, IL, USA) but different plasma unit weight settings (700 g vs. 720 g), we observed a diminished efficiency of the procedure when plasma collection was extended over 700 g [14]. Our observational data suggested that a shift in the setting from 700 g to 720 g was associated with a small increase in net plasma in the final unit (4 mL) but implied an increase of over 100 mL in total blood processed and a higher amount of ACD-A in plasma collected.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, our previous observational study [14] also comprised data from a few women. It is therefore possible to exclude (or confirm) a potential sex effect on the efficiency of plasmapheresis and citrate distribution between unit and donor, and we also recruited female subjects with the plasma donation level set at 700 g.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Plasma Unit Weight and Donor Sex on Post-Donation Citrate Level: An Experimental Study on Plasmapheresis Donors

Chiara

Schiroli

Mancuso

et al. 2023

Transfus Med Hemother

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Introduction: Plasmapheresis donation is considered safe and well tolerated, although long-term effects need to be clarified. The volumes of anticoagulant (ACD-A) used are variable and depend primarily on hematocrit (HCT), total blood processed, amount of plasma collected, and donor characteristics. To elucidate the effect of the plasma unit weight setting on plasmapheresis efficiency and ACD-A distribution, we enrolled male donors undergoing a controlled apheresis process donating 700 g and 720 g in two different sessions. In parallel, we investigated a possible effect of sex, recruiting women donating 700 g of plasma. Methods: The study was conducted on men donating 720 g and (12 months later) 700 g of plasma, and on women donating 700 g of plasma. The main outcomes were pre-/post-donation delta (Δ) citrate concentration in donor plasma and ACD-A reinfused to the donor. Information concerning the annual check-up and the procedure was also collected. Intergroup comparisons (men donating 720 g vs. men donating 700 g and men vs. women both donating 700 g) and intragroup associations with donor and procedural characteristics were reported. Results: With the procedure set at 720 g, the machine processed around 44 mL more whole blood to collect 20 g more plasma, and 720 g donors received around 12 mL more anticoagulant than 700 g donors. Accordingly, Δ citrate concentration was 1.5 times higher (12 μ<sc>m</sc>), with a greater variability observed for 720 g donations. Citrate concentration in the plasma unit was lower in the 720 g group, although not significantly. Comparing outcomes between women and men donating 700 g, we observed higher (and highly variable) Δ citrate and reinfused ACD-A in women, accompanied by lower anticoagulant levels in the unit. Increased Δ citrate is inversely associated with HCT and age in men and with HCT and triglycerides in women. Reinfused ACD-A correlates with HCT in women but not in men. Conclusion: Unit weight setting and sex influence an ACD-A shift from the estimated values toward an increased reinfusion to donor. In parallel, we observed an impact of age and sex on post-donation citrate metabolism. Altogether, these elements should be taken into account for the development of tailored approaches aimed at maintaining similar safety profiles for all donors using different plasmapheresis settings.

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Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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