Optimisation of solid-state fermentation of Aspergillus niger JL-15 for xylanase production and xylooligosaccharides preparation

Abstract

The production of xylanase (XylA) by Aspergillus niger JL-15 in solid-state fermentation (SSF) on orange peel was optimised by the response surface methodology (RSM). The results revealed that four factors had significant effects on the XylA production (P < 0.05), that is the concentrations of the added glycerin and ammonium sulfate, the moisture content, and fermentation time. Exploying orange peel as the solid substrate, maximum xylanase activity (917.7 U/g dry fermentation product) was obtained at 4.2% glycerin, 3.1% (NH 4 ) 2 SO 4 , 61% moisture content, and 73.4-h fermentation, this activity being close to the predicted one and 3.2 times higher than that of the basic medium (218.5 U/g). Optimum temperature and pH for XylA activity were 55°C and pH 5.0, respectively. SDS-PAGE analysis showed that the relative molecular mass of XylA was about 30.0 kDa. XylA exhibited K m and V max values of 9.24 mg/ml and 54.05 μmol/min/ml, respectively. XylA liberated mainly xylotriose from birchwood xylan and wheat bran, respectively. XylA was an endo-acting xylanase with transglycosylation activity, with the ability to hydrolyse, xylobiose, xylotriose, xylotetraose, xylopentaose, and xylohexaose.

Production and characterization of functional wheat bran hydrolysate rich in reducing sugars, xylooligosaccharides and phenolic acids

“…The potential prebiotic (xylose/XOS) was 0.614 mg/mL in 6 h hydrolyzation. The xylanase from various organisms was reported to generate higher concentration of XOS from WB in 24 h, however, we did not use purified enzyme in only 6 h hydrolyzation [ [38] , [39] , [40] ]. There were some unknown peaks shown in Fig.…”

Screening of Fungal Strains for Cellulolytic and Xylanolytic Activities Production and Evaluation of Brewers’ Spent Grain as Substrate for Enzyme Production by Selected Fungi

“…[25][26][27][28][29]. When compared with the xylanase production by SSF reported for other Aspergillus spp., the maximum value of U/mL achieved by A. niger LPB-334 was higher than some fungi [16,[29][30][31][32][33][34] and lower than others [25,35,36] (Table 2). As far as cellulase production is concerned, the maximum value achieved by A. niger LPB 334 was, in most cases, higher [29,32,33,[37][38][39] and only in a few cases lower [26,36] than reported for other Aspergillus strains (Table 2).…”

Revolutionizing Renewable Resources: Cutting-Edge Trends and Future Prospects in the Valorization of Oligosaccharides

“…The autohydrolysis process also accounts for the hydrolysis susceptibility of arabinan, often present in lignocellulosic residues [27]. Optimal conditions for producing xylooligosaccharides (XOS) and arabinooligosaccharides (AROS) differ, reflecting the unique requirements for each type of oligosaccharide [28,29]. Research indicates similar rates of xylan to XOS conversion across various materials, such as corncobs and rice husks, under comparable conditions [30].…”