Optimisation of solid state fermentation of potato peel for the production of cellulolytic enzymes

Santos, Tereza Cristina dos; Gomes, Devson Paulo Palma; Bonomo, Renata Cristina Ferreira; Franco, Marcelo

doi:10.1016/j.foodchem.2011.11.115

Cited by 90 publications

(30 citation statements)

References 33 publications

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“…The ability of Aspergillus niger to produce enzymes that catalyze the hydrolysis of polymers such as cellulose, hemicellulose, and lignin forming the framework of vegetable matrices enables the production of metabolites required for microbial growth. Some of the synthesized enzymes are amyloglucosidase, endoglucanase, endoglucanase complex and exoglucanase, laccase, xylanase, peroxidase and manganese peroxidase (AHMAD et al, 2013;SANTANA et al, 2012;SANTOS et al, 2012;SANTOS et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Effect of Solid State Fermentation on Nutritional Content and Evaluation of Degradability in Cactus Pear

Santos¹,

Diniz²,

Brito³

et al. 2015

Rev. Caatinga

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-The process of protein enrichment of cactus pear (Nopalea cochenillifera (L.) Salm Dyck) by solid state fermentation with the use of Aspergillus niger and Rhyzopus sp. was studied for improving the nutritional value of this cactus species for use as animal feed. The experiments were conducted in the Agroindustrial Waste Laboratory of State University of Southwest Bahia (Brazil). To this end, we have evaluated the effects of biotransformation on the levels of protein, cellulose, hemicellulose, and lignin, as well as the potential degradability. Bioconversion was carried out using cactus pear as the only substrate, without supplementation with nitrogen, mineral and vitamin sources. The fermentation with Aspergillus niger promoted a 78% increase in/of protein content and reductions of cellulose, hemicellulose, and lignin of 40%, 36%, and 28%, respectively. Degradability, in turn, was observed to have increased by 66 % after 240 h. On the other hand, the fermentation with Rhyzopus sp. was less efficient, with a 69% increase in protein content, and reductions in cellulose, hemicellulose, and lignin contents of 30%, 28%, and 18%. In turn, degradability was seen to have increased by 51%. The fermentation of cactus pear by Aspergillus niger and Rhyzopus sp. exhibited the protein enrichment and increased protein degradability of this Cactaceae. Moreover, this is the most ever efficient micro-organism used in bioconversion. Based on the results, bioconversion of cactus is an excellent alternative to ruminant feeding in arid or semi-arid land. . O efeito da fermentação em estado sólido sobre a palma forrageira foi avaliado comparando os teores de proteína, celulose, hemicelulose e lignina, além da degradabilidade potencial, com os valores in natura. A bioconversão foi realizada utilizando como único substrato a palma forrageira e água, sem a suplementação de fontes alternativas de nitrogênio, minerais ou vitaminas. O fungo Aspergillus niger elevou os teores proteicos em aproximadamente 78% e promoveu a redução nos teores de celulose, hemicelulose e lignina em aproximadamente 40%, 36% e 28%, respectivamente. Por sua vez essa biotransformação elevou a degradabilidade potencial em 66% após 240 h. Enquanto que o fungo Rhyzopus sp. demonstrou ser menos eficiência no enriquecimento proteico, com um aumento de aproximadamente 69 %, e a redução no teores de celulose, hemicelulose e lignina de 30%, 28% e 18%. Ainda para este fungo a degradabilidade potencial teve uma elevação de aproximadamente 51%. Portanto a fermentação da palma forrageira com auxílio dos fungos filamentosos Aspergillus niger e Rhyzopus sp. promoveram um enriquecimento nos teores proteicos desta cactacea além de elevar a sua degradabilidade potencial. Com base nesses resultados, a bioconversão da palma forrageira empregando o método de fermentação em estado sólido pode ser uma excelente alternativa na alimentação de ruminantes em terras áridas ou semiáridas.

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Section: Resultsmentioning

confidence: 99%

Effect of Solid State Fermentation on Nutritional Content and Evaluation of Degradability in Cactus Pear

Santos¹,

Diniz²,

Brito³

et al. 2015

Rev. Caatinga

Self Cite

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“…In filamentous fungi, the temperature directly influences spore germination, growth and formation of products. In practically all the solidstate fermentation processes, temperature was a critical factor in enzyme production due to the metabolic heat generated during the reaction, because in addition to the difficulty to mix the solid medium, most of the substrates utilized have low thermal conductivity (SANTOS et al, 2012). In solid-state fermentation, water activity is a critical factor for fungi growth and controlling its activity is essential for bioprocess optimization.…”

Section: Inmentioning

confidence: 99%

Production and Characterization of Cellulolytic Enzymes by Aspergillus Niger and Rhizopus Sp. By Solid State Fermentation of Prickly Pear

et al. 2016

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-Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.Keywords: Filamentous fungi. Response surface methodology. Bioprocesses. Semi-arid region. Enzymatic characterization. PRODUÇÃO E CARACTERIZAÇÃO DE ENZIMAS CELULOLÍTICAS POR ASPERGILLUS

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“…In fact, bio‐refinery industries are seeking alternative non‐edible and cheaper biological materials such as abundantly available agricultural feedstock or by‐products to fulfill the global bioethanol demand . In this study, we studied potato peel residue (PPR) as an advantageous bio‐energy source that could be exploited for bioethanol production as it contains high amounts of starch and cellulose . Worldwide potato production was estimated to 350 million tons in 2012 .…”

Section: Introductionmentioning

confidence: 99%

Optimization of enzymatic hydrolysis and fermentation conditions for improved bioethanol production from potato peel residues

Taher

Fickers

Chniti

et al. 2017

Biotechnology Progress

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The aim of this work was the optimization of the enzyme hydrolysis of potato peel residues (PPR) for bioethanol production. The process included a pretreatment step followed by an enzyme hydrolysis using crude enzyme system composed of cellulase, amylase and hemicellulase, produced by a mixed culture of Aspergillus niger and Trichoderma reesei. Hydrothermal, alkali and acid pretreatments were considered with regards to the enhancement of enzyme hydrolysis of potato peel residues. The obtained results showed that hydrothermal pretreatment lead to a higher enzyme hydrolysis yield compared to both acid and alkali pretreatments. Enzyme hydrolysis was also optimized for parameters such as temperature, pH, substrate loading and surfactant loading using a response surface methodology. Under optimized conditions, 77 g/L of reducing sugars were obtained. Yeast fermentation of the released reducing sugars led to an ethanol titer of 30g/L after supplementation of the culture medium with ammonium sulfate. Moreover, a comparative study between acid and enzyme hydrolysis of potato peel residues was investigated. Results showed that enzyme hydrolysis offers higher yield of bioethanol production than acid hydrolysis. These results highlight the potential of second generation bioethanol production from potato peel residues treated with onsite produced hydrolytic enzymes.

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Optimisation of solid state fermentation of potato peel for the production of cellulolytic enzymes

Cited by 90 publications

References 33 publications

Effect of Solid State Fermentation on Nutritional Content and Evaluation of Degradability in Cactus Pear

Effect of Solid State Fermentation on Nutritional Content and Evaluation of Degradability in Cactus Pear

Production and Characterization of Cellulolytic Enzymes by Aspergillus Niger and Rhizopus Sp. By Solid State Fermentation of Prickly Pear

Optimization of enzymatic hydrolysis and fermentation conditions for improved bioethanol production from potato peel residues

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