Optimisation of process variables for microbial degradation of phenol by binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescens using response surface methodology

Agarry, S. E.; Audu, T. O. K.; Solomon, B. O.

doi:10.1504/ijep.2010.035921

Cited by 11 publications

(20 citation statements)

References 37 publications

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“…Estos nutrientes son fundamentales para el metabolismo celular debido a que incrementan la efectividad de degradación de hidrocarburos (Walworth & Reynolds, 1995). Estos diseños estadísticos han sido utilizados en varios estudios (Annadurai et al, 2008;Agarry, Solomon, & Layokun, 2008;Ghanem, Al-Garni & Al-Shehri, 2009;Sridevi et al, 2011) para mejorar la degradación de fenol por medio de la optimización de nutrientes en combinación con microorganismos. El uso adecuado de estos diseños estadísticos permiten evaluar diferentes condiciones de ensayo para lograr reducir la complejidad de un problema, para este estudio, se identificaron los factores limitantes y se lograron optimizar los mismos, reduciendo el fenol en las concentraciones evaluadas en aguas de producción de hidrocarburos.…”

Section: Discussionunclassified

Biodegradación de fenol en aguas tratadas de la industria petrolera para re-uso en cultivos agrícolas

Pardo-Díaz

Rojas‐Tapias

Roldán

et al. 2017

RBT

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Oil exploitation and the usage of its derivatives have undeniably contributed to the technological advance worldwide. This industrial activity, however, generates several by-products that can threaten environmental sustainability. Seawage, for example, can contain organic pollutants and heavy metals; therefore, its deposition must be preceded by adequate water treatment. In this study, we isolated, characterized, and molecularly identified eight bacteria on the basis of their capability to degrade phenol. First, we determined the rates of bacterial growth and phenol degradation using different concentrations of the aromatic hydrocarbon (500, 800 and 1 200 mg / L), and then used sequential statistical designs to select optimal conditions for its degradation. Results showed that all isolated strains were capable of degrading phenol as the sole carbon source; the degradation kinetics and phenol tolerance, however, widely varied among strains. We chose the strain Pseudomonas sp. Sps1 for further studies due to its remarkable tolerance and capability to degrade phenol. By using a Plackett Burman design, followed by a fractionated factorial design in which several carbon, nitrogen, and phosphorus sources were evaluated, and phenol degradation was used as the response variable, we found optimal conditions for phenol degradation by Sps1. Finally, we compared phenol degradation in seawage by the use or not of the optimal conditions established by the statistical methods. Results showed that biostimulation dramatically increased phenol degradation compared with the control. In conclusion, we found that bioaugmentation with the native bacteria Sps1 and a statistically-based biostimulation approach provided an economically and environmentally friendly alternative for the removal of pollutants from oil industry sewage.

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Section: Discussionunclassified

Biodegradación de fenol en aguas tratadas de la industria petrolera para re-uso en cultivos agrícolas

Pardo-Díaz

Rojas‐Tapias

Roldán

et al. 2017

RBT

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“…These factors were found critical in biodegradation processes by many previous studies (Menale et al, 2012;Bhattacharya and Banerjee, 2008;El-Naas et al, 2010a;2009;2010b;Sheeja and Murugesan, 2002;Bhattacharya et al, 2009;Agarry et al, 2008b;Yao et al, 2009;Agarry et al, 2008a). Subsequently, this was followed by optimization of the first three factors by the response surface methodology.…”

Section: Single Factor Batch Experiments To Identify the Optimum Rangmentioning

confidence: 99%

“…The enzymatic activity and rate of metabolism are highly dependent on temperature, which makes it an important quantity in bioprocesses (Agarry et al, 2008b). Experiments were carried out to assess the effect of temperature in the range from 22°C (room temperature) to 40°C in two phases as shown in Fig.…”

Section: Effect Of Temperaturementioning

confidence: 99%

“…It is believed that the decrease in the reactivity of the enzyme system within the cell, that is usually responsible for ring cleavage, will result in a decrease in the degradation rate (Agarry et al, 2008b;Sheeja and Murugesan, 2002). Additionally, extreme temperatures lower the water solubility of oxygen resulting in insufficient content to favor a higher reaction rate at high temperatures (Menale et al, 2012).…”

Section: Effect Of Temperaturementioning

confidence: 99%

“…Biotreatment process optimization by statistical experimental design has been well documented (Annadurai et al, 2008;Sridevi et al, 2011;Sheeja and Murugesan, 2002;Wu et al, 2009;Rigas et al, 2005;Cutright and Meza, 2007;Agarry et al, 2008b;Yao et al, 2009). However few studies were reported as to chlorophenols removal (Kusic et al, 2011;Bhattacharya et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of 2, 4 Dichlorophenol

Al-Khalid¹,

El‐Naas²

2017

American Journal of Engineering and Applied Sciences

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Abstract:The present study focuses on the optimization of process parameters of the aerobic biodegradation of 2, 4 Dichlorophenol (2, 4 DCP) by a commercial strain of P. putida immobilized in PVA gel matrix, using design of experiments by Response Surface Methodology (RSM). The degradation rates obtained from these experiments were used to evaluate the effects of the main factors and their interactions during the biodegradation of 2, 4 DCP. An effective quadratic regression model predicting the rate in terms of the main independent variables, namely temperature, initial pH and initial concentration of 2, 4 DCP, was developed. The optimum conditions for DCP degradation were obtained as follows: Temperature 32.6°C, pH 5.0 and initial DCP concentration 70.5 mg L −1 , resulting in a maximum predicted degradation rate of 41.8 mg L. Under these optimized conditions, a degradation rate of 40.1 mg L −1 h −1was experimentally obtained, thus validating the model.

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Experimental Investigation on Abstraction of Phenol Onto Micrococcus lylae and Cetyl Trimethyl Ammonium Bromide

Saravani

Senthilkumar

Kugarajah

2016

CLEAN Soil Air Water

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Experimental Investigation on Abstraction of Phenol Onto Micrococcus lylae and Cetyl Trimethyl Ammonium BromideAir floatation, an efficient solid-liquid separation process, was tested as a post treatment technique for phenol removal. The results consider about the practical solutions for certain operations such as efficient solid-liquid separation, attainment of maximized percentage removal, phenol rich solution with Micrococcus lylae cells, and pure water without M. lylae cells. The two processes, biosorption and flotation, can effectively operate in combination and both the sorbent and treated water can be recycled. The effect of various operating parameters, such as initial feed concentration, equilibrium time, biosorbent dose, pH, liquid pool height, surfactant concentration, and air flow rate, was experimentally investigated. The maximum sorption capacity was found to be 303 mg/g at pH 7 and an initial phenol concentration of 500 mg/L. The higher floatability of M. lylae was obtained in a maximum time of 10 min. In addition, the adsorption isotherm and kinetic studies revealed that the biosorption process followed the Dubinin-Radushkevich model (R 2 ¼ 0.982) and pseudo-first order kinetics with a kinetic constant of 2.6537/day. The adsorbed chemical species was identified by Fourier transform infrared (FTIR) spectroscopy. Electrokinetic measurements were carried out to determine the isoelectric point of the bacteria. The zeta potential profile of the bacteria was affected by the presence of phenol at different pH values. The recovery of phenol loaded biomass and final traces of phenol by flotation were found to be 99.91%.Abbreviations: 4-AAP, 4-amino anti pyrine; BET, Brunauer-Emmett-Teller; CTAB, cetyl trimethyl ammonium bromide; FTIR, Fourier transform infrared.

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Optimisation of process variables for microbial degradation of phenol by binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescens using response surface methodology

Cited by 11 publications

References 37 publications

Biodegradación de fenol en aguas tratadas de la industria petrolera para re-uso en cultivos agrícolas

Biodegradación de fenol en aguas tratadas de la industria petrolera para re-uso en cultivos agrícolas

Biodegradation of 2, 4 Dichlorophenol

Experimental Investigation on Abstraction of Phenol Onto Micrococcus lylae and Cetyl Trimethyl Ammonium Bromide

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