Optimisation of nutritional requirements for dopamine synthesis by calcium alginate-entrapped mutant strain of<i>Aspergillus oryzae</i>EMS-6

Ali, Sikander; Nawaz, Wajeeha

doi:10.1080/14786419.2016.1233408

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…There are not significant differences between normal group and other groups in ALP value. These result agree with Ali and Nawaz, (2017) who recorded that measure the level of serum liver enzymes are commonly referred to as liver function tests, they usually reflect hepatocyte integrity or cholestasis rather than liver function is specific for liver disease,…”

Section: Characterization Of Groupssupporting

confidence: 91%

Assessment of Aflatoxins in Some Foods and Its Relation to Hepatic Diseases

Sayed²,

et al. 2018

Journal of Environmental Science

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Humans are exposed to hepatocarcinogenic aflatoxins (Afs) through ingestion of contaminated foods as a result of poor storageful of susceptible grains or eating foods contaminated with aflatoxins animals and vegetables. This work aimed at evaluating the effect level of total (Afs) on human health. Study samples ninety persons divided into three groups (each /30 human). Group (1) no suffering liver disease as normal or negative control. Group (2) patients input (El Raghi hospital in Assuit) are suffering of liver inflammatory disease (HI). Group (3) patients input/or output (El Raghi hospital in Assuit) are suffering of hepatocellular carcinoma (HCC). Random samples were obtained from some foods (chicken egg; chicken, duck and rabbit liver) purchased from local market in Assuit. Total amount of aflatoxins was determined by using high-performance liquid chromatography (HPLC) instrumental. This study used 24 h recall and food frequency questioners for these groups to determination the type and amount of food which were intake. In serum humans measured total aflatoxins by used the enzyme-linked immunosorbent assay (ELISA) kits and ELISA technique. In addition oxidation tests are carried out for the studied individuals by using colorimetric kites to determine liver function (Alanine transaminase (ALT), Aspartate transaminase (AST), Total protein (TP),albumin (Alb), Bilirubin (Total, Direct), alkaline phosphatase (ALP), Glutathione peroxidase (GPX) and Malondialdehyde (MDA) by used spectroscopy instrument. Results indicated that were significant increase in liver function and biomarker antioxidants (GPX, MDA). Study concluded that these alterations in liver functions could be related to the development of liver damage in

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Section: Characterization Of Groupssupporting

confidence: 91%

Assessment of Aflatoxins in Some Foods and Its Relation to Hepatic Diseases

Sayed²,

et al. 2018

Journal of Environmental Science

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“…Azin and Noroozi ( 2001 ) also reported increased enzyme activity with NA mutagenesis in Aspergillus oryzae PTCC 5164. Whereas, Ali and Nawaz ( 2017 ) also observed resistance development in Aspergillus oryzae mutant with low L-cysteine HCl concentration.…”

Section: Discussionmentioning

confidence: 98%

Kinetics and synthesis of poly(3-hydroxybutyrate) by a putative-mutant of Bacillus licheniformis

Ali,

Lodhi,

Ahmad

et al. 2024

Bioresour. Bioprocess.

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The present study deals with the kinetics of improved poly(3-hydroxybutyrate) (PHB) production by an L-cysteine HCl-depressed mutant of Bacillus licheniformis. Production of biodegradable polymers is to eliminate use of materials derived from petrochemicals and also because of their environmental impact. For the current study, mutant strain (NA-21) & wild-type (IIB-isl19) were used for PHB production. Submerged culture with two-stage fermentation technique was used for PHB production. Results indicated that PHB production was improved with 300 mM of –HNO2. The superior mutant strain (NA-21) resulted in 2-fold more PHB as compared to the wild-type (IIB-isl9). It was selected, and resistance against L-cysteine HCl was developed. At 4 ppm concentration of L-cysteine HCl, PHB production by mutant strain (NA-cys4) was higher than its wild counterpart by 5.7-fold. Kinetic study of parameters including specific growth rate (µ h− 1), growth (Yx/s,Ys/x), product yield coefficients (Yp/s,Yp/x), volumetric rate constants (Qp, Qs, Qx) and specific rate constants (qp, qs, qx), were also accomplished. Moreover, Yp/x, Qp and qp = µ × Yp/x were found to be very significant as 1.254 ± 0.06 (g/g biomass), 0.134 ± 0.01 (g/l/h) and 0.168 ± 0.01 (g/g/h), respectively. The effect of fatty acids on PHB production highlighted the improvement in PHB production by 1.94-fold. The highest PHB production during the study was 16.35 ± 3.12 g/l which highlighted its significance (p ≤ 0.05) and impact on the overall process. The variation in PBH yield between wild-type and mutant B. licheniformis is possibly because of induced DNA interstrand thus making unstable thymidine-thymidine dymers. From the results, it was concluded that improved PHB production on industrial scale is fairly possible and it holds the potential to contribute significantly to plastic circularity in the future.

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Immobilization of Aspergillus oryzae tyrosine hydroxylase on ZnO nanocrystals for improved stability and catalytic efficiency towards L-dopa production

Khalid,

Ali,

Rukhma

et al. 2023

Sci Rep

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The current study focuses on the submerged fermentation of tyrosine hydroxylase (TH) from Aspergillus oryzae IIB-9 and its immobilization on zinc oxide nanocrystals (ZnO-NPs) for increased L-dopa production. The volume of Vogel’s medium (75 ml), period of incubation (72 h), initial pH (5.5), and size of inoculum (1.5 ml) were optimal for maximum TH activity. The watch glass-dried (WG) and filter paper-dried (FP) ZnO-NPs were prepared and characterized using analytical techniques. The UV–Vis spectra revealed 295 and 285 nm absorption peaks for WG-ZnO-NPs and FP-ZnO-NPs dispersed in isopropanol. X-ray diffraction analysis confirmed the crystalline nature of ZnO-NPs. FTIR spectra band from 740 to 648.1/cm and 735.8/cm to 650.1/cm showed the stretching vibrations of WG-ZnO-NPs and FP-ZnO-NPs, respectively. The particle size of ZnO-NPs observed by scanning electron microscopy (SEM) images was between 130 and 170 nm. Furthermore, the stability of immobilized TH on ZnO-NPs was determined by varying the incubation period (10 min for WG-NPs and 15 min for FP-NPs) and temperature (45 °C and 30 °C for WG and FP-NPs, respectively). Incubating enzymes with various copper, iron, manganese, and zinc salts studied the catalytic efficiency of TH. Immobilization of TH on ZnO-NPs resulted in an 11.05-fold increase in TH activity, thus enhancing stability and catalytic efficiency.

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Optimisation of nutritional requirements for dopamine synthesis by calcium alginate-entrapped mutant strain ofAspergillus oryzaeEMS-6

Cited by 4 publications

References 20 publications

Assessment of Aflatoxins in Some Foods and Its Relation to Hepatic Diseases

Assessment of Aflatoxins in Some Foods and Its Relation to Hepatic Diseases

Kinetics and synthesis of poly(3-hydroxybutyrate) by a putative-mutant of Bacillus licheniformis

Immobilization of Aspergillus oryzae tyrosine hydroxylase on ZnO nanocrystals for improved stability and catalytic efficiency towards L-dopa production

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