Optimisation of liquid chromatographic performance on columns packed with microparticulate silicas

Citreoviridin, a specific inhibitor of the mitochondiral adenosine triphosphatase

“…A stainless-steel column (25cm x 0.95 cm) packed with 10gm-diameter silica gel (Partisil 10; Whatman, Maidstone, Kent, U.K.) was used as described by Webber & McKerrell (1976). A pressure of about 3.8 MPa (4001b/in2) gave a flow rate of 4-5ml/min.…”

Efficient packing of small particle microbore columns

“…Numerous procedures for packing conventional columns (3.9-5.0 mm i.d.) have been proposed over the past decade, including balanced density (I, 2), high viscosity (3,4), and dilute slurry (5) techniques. Packing methods for reversed phase have been advocated in which sluury solvents have varied from alcohols (6)(7)(8) and halogenated solvents (9) to organic acids (10).…”

“…Effective slurry concentrations have ranged from 5 to 30% w/v. Columns have been packed at constant pressure (ranging from 3000 to 10000 psi or more) (1,2,8) and constant velocity (3,9), both upward (7,8,11) and downward (1,(4)(5)(6).…”

Some Applications of Electrochemical Oxidation as a Detection Technique in High Performance Liquid Chromatography

“…Other solvents were of reagent grade and were used without further treatment. The HPLC columns were constructed from 0.10 m or 0.20 m x 4.5 mm ID Apollo liquid chromatography stainless steel tubing (Magnus Scientific, Sandbach, Great Britain) and were packed in the laboratory using a carbon tetrachloride slurry technique [4] at a packing pressure of 31.0 MPa (4500 psig).…”