Optimisation of gene transfer into vascular endothelial cells using electroporation

Abstract

The results indicate that endothelial cells can be efficiently transduced by electroporation to stably express an introduced gene. This may have important implications in vascular surgery.

Prediction and optimization of gene transfection and drug delivery by electroporation

“…We first tested the ability to predict relative levels of DNA transfection for 34 different mammalian cell types from 12 different studies [17][18][19][20][21][22][23][24][25][26][27][28] (See Materials and methods for discussion of how these predictions were made). Of greatest importance for gene therapy applications is the ability to predict the set of electroporation conditions corresponding to the maximum level of transfection.…”

“…11,[17][18][19]24,25,[27][28][29][30][31][32][33][34][35][36][37][38] To validate the correlation, we compared experimental data to predictions of electroporation conditions that yield 50% viability, since this criterion is often used as a 'rule of thumb' to crudely identify optimal laboratory transfection conditions. [1][2][3][4] Overall, predictions for 50% viability were within an average of 29 ± 28% of experimentally determined values (eg Figure 2a,c).…”

Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse

“…Electroporation-mediated gene transfer has been widely used to introduce DNA into various types of cells in vitro, [7][8][9][10][11] and in vivo application of electronic pulsemediated gene transfer, using specially designed electrodes, has been shown to be effective for mouse muscle, 12,13 chick embryos, 14 rat liver, 15 cardiac tissue, 16 and solid tumors. [17][18][19] Information on the usefulness of electroporation in arterial gene transfer has not been available.…”

Ex vivo electroporation as a potent new strategy for nonviral gene transfer into autologous vein grafts

“…The midportion of each vein graft was sliced into four segments at 5-mm intervals, embedded in paraffin, sectioned to 5 m, and stained with hematoxylin-eosin or Elastica van Gieson. Intimal thickness (defined according to the 8-points method) and the square of the intima were measured by MAC SCOPE software (Mitani, Fukui, Japan) as described previously (29,35,20).…”