2010
DOI: 10.1007/s10695-010-9401-z
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Optimisation of gene expression analysis in Atlantic salmon lenses by refining sampling strategy and tissue storage

Abstract: Analysis of gene expression in the lens is one of the analytical tools employed to investigate cataract formation in Atlantic salmon (Salmo salar L.). High quality RNA preparations are an essential prerequisite for gene expression analysis. The first aim of the present study was to investigate the possible effects of two methods of tissue preservation on the quality of RNA extracted from Atlantic salmon lenses. RNA was extracted from lenses either stored in RNAlater or flash-frozen in liquid nitrogen. Both tis… Show more

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Cited by 5 publications
(2 citation statements)
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References 25 publications
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“…At the end of the experiment, eight fish per treatment were randomly captured and sacrificed according to the AVMA recommendations (Underwood et al 2013) with an overdose of benzocaine. The liver and distal intestine were extracted in cold under aseptic conditions, 50 mg of each tissue stored in RNAlater (Sigma Aldrich, Saint Louis, MI, USA), kept at 4°C overnight, and subsequently stored at -80°C until further processing and analysis (Tröβe et al 2010).…”
Section: Samplingmentioning
confidence: 99%
“…At the end of the experiment, eight fish per treatment were randomly captured and sacrificed according to the AVMA recommendations (Underwood et al 2013) with an overdose of benzocaine. The liver and distal intestine were extracted in cold under aseptic conditions, 50 mg of each tissue stored in RNAlater (Sigma Aldrich, Saint Louis, MI, USA), kept at 4°C overnight, and subsequently stored at -80°C until further processing and analysis (Tröβe et al 2010).…”
Section: Samplingmentioning
confidence: 99%
“…Data in salmon demonstrate that sedation of 30 min to 2 h before euthanasia and tissue sampling resulted in differential regulation of fast-responding genes. 9,10 Data in rodents are mostly limited to comparison of anesthesia effects during experimental induction of cerebral pathologies. 6,11 There is evidence, however, that the use of isoflurane for euthanasia induces immediate changes in electrophysiology and neuroplasticity compared with decapitation without anesthesia or decapitation with anesthesia 12 ; and that isoflurane anesthesia before CO 2 euthanasia increased c-fos expression in mice brains.…”
Section: Discussionmentioning
confidence: 99%