Optimisation of a pollen DNA metabarcoding method for diet analysis of flying-foxes (

Bell, Karen L.; Batchelor, Kathryn L.; Bradford, Matt; McKeown, Adam; Macdonald, Stewart L.; Westcott, David A.

doi:10.1071/zo20085

Cited by 15 publications

(21 citation statements)

References 45 publications

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“…Complete methods for the extraction of DNA from samples and the identification of plant taxa are provided in Bell et al . 2021.…”

Section: Resultsmentioning

confidence: 99%

“…Complete methods for the extraction of DNA from samples and the identification of plant taxa are provided in Bell et al . (2021). Briefly, we used a DNA barcode from the ITS2 region (Chen et al .…”

Section: Methodsmentioning

confidence: 99%

“…Complete methods for the extraction of DNA from samples and the identification of plant taxa are provided in Bell et al (2021). Briefly, we used a DNA barcode from the ITS2 region (Chen et al 2010) sequenced using Illumina MiSeq and analysed the data using a bioinformatics pipeline based on the dada2 package (Callahan et al 2016), with taxonomic matching to a database assembled from publicly available ITS2 data (www.ncbi.nlm.gov) supplemented with identified leaf samples collected from the field.…”

Section: Faecal Edna Metabarcodingmentioning

confidence: 99%

“…A full list of diet species from all sources combined is presented in Appendix S1. Complete methods for the extraction of DNA from samples and the identification of plant taxa are provided inBell et al 2021.…”

mentioning

confidence: 99%

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The diet of a specialist nectarivore in Australia: The little red flying‐fox (Pteropus scapulatus, Pteropodidae)

et al. 2022

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The little red flying-fox (Pteropus scapulatus, Pteropodidae) is the most widely distributed of the four Australian mainland flying-fox (Pteropus) species. They move very large distances following foraging resources and congregate in large numbers which often causes human-animal conflict. To better understand the resources that drive these movements and aggregations, we investigated the diet of the little red flying-fox using data from five sources: (i) faecal eDNA metabarcoding of field sampled little red flying-foxes; (ii) identification of foraging locations through satellite tracking; (iii) a literature search; (iv) a search of online databases; and (v) an expert survey. Our sources revealed a specialist nectarivore diet containing 204 species, dominated by floral products from the plant family Myrtaceae. We consider a small number of widely occurring and structurally dominant Myrtaceae, particularly from the genera Corymbia, Eucalyptus and Melaleuca, as major diet species that regularly drive mass aggregations. In addition, we consider a moderate number of species dominated by the Myrtaceae as important diet species and a large number of species from diverse taxa as supplementary diet species. Fruit represents approximately 5% of the diet suggesting that the little red flying-fox is unlikely to be a major pest of horticultural crops or disperser of weeds. The combination of long-distance movement and a wide range of diet species results in a long-distance pollination service to many plant species which likely promotes genetic mixing between isolated populations of plants. Our understanding of the little red flying-fox diet allows us to better predict mass migrations and aggregations at a continental scale and allows us to clearly identify key foraging habitat so that informed management decisions can be made.

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“…Complete methods for the extraction of DNA from samples and the identification of plant taxa are provided in Bell et al . 2021.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Faecal Edna Metabarcodingmentioning

confidence: 99%

mentioning

confidence: 99%

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The diet of a specialist nectarivore in Australia: The little red flying‐fox (Pteropus scapulatus, Pteropodidae)

et al. 2022

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“…For example, the first national DNA barcode sequence reference database of Wales [141] has provided a benchmark for DNA metabarcoding studies of plantpollinator interactions and this has recently grown into a comprehensive database for all of the United Kingdom [59]. Together, these databases have proven powerful for reconstructing bee foraging behavior [142][143][144]. These and other studies [145][146][147] have built a strong foundation for using DNA metabarcoding to study plant-pollinator interactions.…”

Section: Symbiotic Relationships and Plant-pollinator Interactionsmentioning

confidence: 99%

The Expanding Role of DNA Barcodes: Indispensable Tools for Ecology, Evolution, and Conservation

Gostel

Kress

2022

Diversity

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DNA barcoding has transformed the fields of ecology, evolution, and conservation by providing a rapid and effective tool for species identification. The growth of DNA barcodes as a resource for biologists has followed advances in computational and sequencing technology that have enabled high-throughput barcoding applications. The global DNA barcode database is expanding to represent the diversity of species on Earth thanks to efforts by international consortia and expanding biological collections. Today, DNA barcoding is instrumental in advancing our understanding of how species evolve, how they interact, and how we can slow down their extirpation and extinction. This review focuses on current applications of DNA barcode sequences to address fundamental lines of research, as well as new and expanding applications of which DNA barcoding will play a central role.

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Using eDNA metabarcoding to understand the effect of fire on the diet of small mammals in a woodland ecosystem

Wanniarachchi

Swan

Nevill

et al. 2022

Ecology and Evolution

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Food acquisition is a fundamental process that drives animal distribution and abundance, influencing how species respond to changing environments. Disturbances such as fire create significant shifts in available dietary resources, yet, for many species, we lack basic information about what they eat, let alone how they respond to a changing resource base. In order to create effective management strategies, faunal conservation in flammable landscapes requires a greater understanding of what animals eat and how this change following a fire. What animals eat in postfire environments has received little attention due to the time-consuming methodologies and low-resolution identification of food taxa. Recently, molecular techniques have been developed to identify food DNA in scats, making it possible to identify animal diets with enhanced resolution. The primary aim of this study was to utilize eDNA metabarcoding to obtain an improved understanding of the diet of three native Australian small mammal species: yellow-footed antechinus (Antechinus flavipes), heath mouse (Pseudomys shortridgei), and bush rat (Rattus fuscipes). Specifically, we sought to understand the difference in the overall diet of the three species and how diet changed over time after fire. Yellow-footed antechinus diets mostly consisted of moths, and plants belonging to myrtles and legume families while bush rats consumed legumes, myrtles, rushes, and beetles. Heath mouse diet was dominated by rushes. All three species shifted their diets over time after fire, with most pronounced shifts in the bush rats and least for heath mice. Identifying critical food resources for native animals will allow conservation managers to consider the effect of fire management actions on these resources and help conserve the species that use them.

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Optimisation of a pollen DNA metabarcoding method for diet analysis of flying-foxes (

Cited by 15 publications

References 45 publications

The diet of a specialist nectarivore in Australia: The little red flying‐fox (Pteropus scapulatus, Pteropodidae)

The diet of a specialist nectarivore in Australia: The little red flying‐fox (Pteropus scapulatus, Pteropodidae)

The Expanding Role of DNA Barcodes: Indispensable Tools for Ecology, Evolution, and Conservation

Using eDNA metabarcoding to understand the effect of fire on the diet of small mammals in a woodland ecosystem

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