Optimisation of a mycobacterial replicon increases foreign antigen expression in mycobacteria

Griffin, Sarah; Williamson, Anna‐Lise; Chapman, Rosamund

doi:10.1016/j.tube.2009.03.006

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Cited by 5 publications

(1 citation statement)

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“…However, using a more reliable quantitative PCR (qPCR) method, the copy number in M. smegmatis was shown to be ϳ23 (44). The expression levels of proteins vary, depending on the strain, suggesting that the copy number of the vector is also dependent on the host strain (45). When expressing genes that are detrimental to cell growth if overexpressed, it is disadvantageous to express them from multicopy episomal plasmids.…”

Section: Resultsmentioning

confidence: 99%

Development of a New Generation of Vectors for Gene Expression, Gene Replacement, and Protein-Protein Interaction Studies in Mycobacteria

Parikh

Kumar

Chawla

et al. 2013

Appl Environ Microbiol

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Section: Resultsmentioning

confidence: 99%

Development of a New Generation of Vectors for Gene Expression, Gene Replacement, and Protein-Protein Interaction Studies in Mycobacteria

Parikh

Kumar

Chawla

et al. 2013

Appl Environ Microbiol

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Recombinant BCG vaccines: molecular features and their influence in the expression of foreign genes

Oliveira

Rizzi

Dellagostin

2017

Appl Microbiol Biotechnol

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Recombinant Mycobacterium bovis BCG vaccines (rBCG) were first developed in the 1990s as a means of expressing antigens from multiple pathogens. This review examines the key structural factors of recombinant M. bovis that influence the expression of the heterologous antigens and the generation of genetic and functional stability in rBCG, which are crucial for inducing strong and lasting immune responses. The fundamental aim of this paper is to provide an overview of factors that affect the expression of recombinant proteins in BCG and the generation of the immune response against the target antigens, including mycobacterial promoters, location of foreign antigens, and stability of the vectors. The reporter systems that have been employed for evaluation of these molecular features in BCG are also reviewed here.

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Construction and evaluation of pMycoFos, a fosmid shuttle vector for Mycobacterium spp. with inducible gene expression and copy number control

Liew

et al. 2011

Journal of Microbiological Methods

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Optimisation of a mycobacterial replicon increases foreign antigen expression in mycobacteria

Cited by 5 publications

References 35 publications

Development of a New Generation of Vectors for Gene Expression, Gene Replacement, and Protein-Protein Interaction Studies in Mycobacteria

Development of a New Generation of Vectors for Gene Expression, Gene Replacement, and Protein-Protein Interaction Studies in Mycobacteria

Recombinant BCG vaccines: molecular features and their influence in the expression of foreign genes

Construction and evaluation of pMycoFos, a fosmid shuttle vector for Mycobacterium spp. with inducible gene expression and copy number control

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