Optimisation of a direct plating method for the detection and enumeration of Alicyclobacillus acidoterrestris spores

Henczka, Marek; Djas, Małgorzata; Filipek, Katarzyna

doi:10.1016/j.mimet.2012.10.007

Cited by 11 publications

(8 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditional methods for detecting A. acidoterrestris are based on selective enrichment and subsequent culture on a selective medium, which are laborious and take up to 3-5 days to give a positive result [8]. To expeditiously detect this bacterium in food samples, a number of rapid methods have been developed, including polymerase chain reaction (PCR), Enzyme-linked immunosorbent assay (ELISA), Fourier transform infrared (FT-IR), electronic nose and 16S rDNA gene sequence analysis [4,9–11].…”

Section: Introductionmentioning

confidence: 99%

Immunomagnetic Separation Combined with Polymerase Chain Reaction for the Detection of Alicyclobacillus acidoterrestris in Apple Juice

Wang

Yue

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

A combination of immunomagnetic separation (IMS) and polymerase chain reaction (PCR) was used to detect Alicyclobacillus acidoterrestris (A. acidoterrestris) in apple juice. The optimum technological parameters of the IMS system were investigated. The results indicated that the immunocapture reactions could be finished in 60 min and the quantity of IMPs used for IMS was 2.5 mg/mL. Then the combined IMS-PCR procedure was assessed by detecting A. acidoterrestris in apple juice samples. The agarose gel electrophoresis results of 20 different strains showed that the IMS-PCR procedure presented high specificity to the A. acidoterrestris. The sensitivity of the IMS-PCR was 2×101 CFU/mL and the total detection time was 3 to 4 h. Of the 78 naturally contaminated apple juice samples examined, the sensitivity, specificity and accuracy of IMS-PCR compared with the standardized pour plate method were 90.9%, 97.0% and 96.2%, respectively. The results exhibited that the developed IMS-PCR method will be a valuable tool for detecting A. acidoterrestris and improving food quality in juice samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Immunomagnetic Separation Combined with Polymerase Chain Reaction for the Detection of Alicyclobacillus acidoterrestris in Apple Juice

Wang

Yue

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…have become a major concern to the global juice and beverage industries [ 1 , 4 , 9 ]. Currently, the culture-based techniques are mainly used as routine analysis of fruit juice products in world famous enterprises [ 2 , 9 ]. The development of rapid and reliable technique for the detection of Alicyclobacillus spp.…”

Section: Discussionmentioning

confidence: 99%

“…Alicyclobacillus spp. are one of the most important quality and safety factors in acidic food products [ 1 , 2 ]. The pasteurization processes are not effective against the thermo-acidophilic spore-forming microorganisms and their spores can survive conventional heat processes.…”

Section: Introductionmentioning

confidence: 99%

Detection of Alicyclobacillus spp. in Fruit Juice by Combination of Immunomagnetic Separation and a SYBR Green I Real-Time PCR Assay

et al. 2015

View full text Add to dashboard Cite

An approach based on immunomagnetic separation (IMS) and SYBR Green I real-time PCR (real-time PCR) with species-specific primers and melting curve analysis was proposed as a rapid and effective method for detecting Alicyclobacillus spp. in fruit juices. Specific primers targeting the 16S rDNA sequences of Alicyclobacillus spp. were designed and then confirmed by the amplification of DNA extracted from standard strains and isolates. Spiked samples containing known amounts of target bacteria were used to obtain standard curves; the correlation coefficient was greater than 0.986 and the real-time PCR amplification efficiencies were 98.9%- 101.8%. The detection limit of the testing system was 2.8×101 CFU/mL. The coefficient of variation for intra-assay and inter-assay variability were all within the acceptable limit of 5%. Besides, the performance of the IMS-real-time PCR assay was further investigated by detecting naturally contaminated kiwi fruit juice; the sensitivity, specificity and accuracy were 91.7%, 95.9% and 95.3%, respectively. The established IMS-real-time PCR procedure provides a new method for identification and quantitative detection of Alicyclobacillus spp. in fruit juice.

show abstract

“…59 acidoterrestris in fruit juice industry. However, they usually require 3-5 days to 60 generate positive results, which is too long to minimize the financial losses for juice 61 producers (Henczka, Djas, & Filipek, 2013 Table 2 and Table 3 were synthesized by 143 Takara Biotechnology (Dalian, China). Fig.…”

mentioning

confidence: 99%

A novel developed method based on single primer isothermal amplification for rapid detection of Alicyclobacillus acidoterrestris in apple juice

Yang

et al. 2017

Food Control

View full text Add to dashboard Cite

Optimisation of a direct plating method for the detection and enumeration of Alicyclobacillus acidoterrestris spores

Cited by 11 publications

References 18 publications

Immunomagnetic Separation Combined with Polymerase Chain Reaction for the Detection of Alicyclobacillus acidoterrestris in Apple Juice

Immunomagnetic Separation Combined with Polymerase Chain Reaction for the Detection of Alicyclobacillus acidoterrestris in Apple Juice

Detection of Alicyclobacillus spp. in Fruit Juice by Combination of Immunomagnetic Separation and a SYBR Green I Real-Time PCR Assay

A novel developed method based on single primer isothermal amplification for rapid detection of Alicyclobacillus acidoterrestris in apple juice

Contact Info

Product

Resources

About