OPTIMASI NAA DAN BAP TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TUNAS MIKRO TANAMAN KANTONG SEMAR (Nepenthes mirabilis) SECARA IN VITRO

Rosmaina, Rosmaina; Aryani, Dinni

doi:10.24014/ja.v5i2.1352

Cited by 6 publications

(7 citation statements)

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“…Kultur in vitro adalah metode mengisolasi bagian dari tanaman seperti protoplasma, sel, jaringan dan organ, serta menumbuhkannya dalam kondisi aseptik, sehingga bagian tanaman tersebut bisa memperbanyak diri dan beregenerasi menjadi tanaman lengkap (Rosmaina & Aryani, 2015).…”

Section: Pendahuluanunclassified

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Nurussakinah¹,

Dwiati²,

Budisantoso³

2022

bioe

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Nepenthes sp. memiliki ciri khas berupa ujung daun mengalami modifikasi menjadi kantong. Nepenthes mirabilis (Lour.) Druce dihabitat aslinya banyak diburu untuk diperjualbelikan. Perbanyakan N. mirabilis di alam sangat kecil tingkat keberhasilannya, untuk mengatasi hal tersebut perlu dilakukan perbanyakan dengan cara kultur in vitro dan menggunakan eksplan daun muda untuk membentuk kalus. Zat pengatur tumbuh seperti Auksin (NAA). Sitokinin (TDZ) dapat memacu pertumbuhan kalus. Auksin dapat merangsang pembentukan kalus dengan memacu pembentangan sel dan Sitokinin dapat memacu proses pembelahan sel. Tujuan penelitian ini untuk mengetahui pengaruh NAA dan TDZ dalam menginduksi kalus N. mirabilis dan mengetahui Interaksi antara NAA dan TDZ dalam menginduksi kalus N. mirabilis. Rancangan percobaan yang digunakan adalah RAL pola Faktorial, dengan dua faktor, yaitu NAA dan TDZ. NAA terdiri atas empat taraf yakni, 0 mg/L (N0); 0,5 mg/L (N1), 1 mg/L (N2) dan 1,5 mg/L (N3). TDZ juga terdiri atas empat taraf yakni 0 mg/L (T0); 1 mg/L (T1); 2 mg/L (T2) dan 3 mg/L (T3). Masing-masing perlakuan diulang sebanyak tiga kali. Hasil penelitian menunjukkan bahwa penambahan NAA dan TDZ berpengaruh terhadap induksi kalus N. mirabilis. Tidak terdapat interaksi antara NAA dan TDZ dalam menginduksi kalus. Konsentasi TDZ 1 mg/L (T1) memacu induksi kalus khususnya persentase pembentukan kalus dan diameter kalus. Sementara itu, NAA 1 mg/L (N2) memacu persentase pembentukan kalus N. mirabilis.

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Section: Pendahuluanunclassified

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Nurussakinah¹,

Dwiati²,

Budisantoso³

2022

bioe

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“…Pemindahan tunas dapat dilakukan secara individu atau berkelompok. Pemanjangan tunas secara berkelompok lebih ekonomis daripada secara individu, dimana pemanjangan tunas bertujuan untuk pengakaran yang umumnya memerlukan hormon auksin seperti NAA atau IBA (Alitalia, 2008). Hal ini ditunjukkan pada Gambar 3.…”

Section: Tahap Pemanjangan Tunas Induksi Dan Perkembangan Akarunclassified

POTENSI DAN ANALISA BIAYA PENYEDIAAN BIBIT TEBU (Saccharum officinarum L.) UNGGUL SECARA IN VITRO DI PT. GUNUNG MADU PLANTATIONS

Sarı¹,

Mahfut²,

Ahyar³

2022

Biolokus

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<p><em>Sugarcane productivity included cane yield and sugar extracted rate (rendemen) fluctuated annually. The main causes of low sugarcane productivity is quality of planting material with no high sugar extracted rate (sugar rendemen) potential, resistance to pests and diseases, stability production, tolerance to climate changed and early age of harvest. For increasing of the yield productivity and producing a superior sugarcane planting material with competitiveness cost, the vegetative propagation through in vitro culture technique via shoot tip culture was carried out. The objective of this research is to produce a superior sugarcane planting in vitro and analyze the costs required. The research was carried out at the Tissue Culture Laboratory, PT. Gunung Madu Plantations (GMP) in March-May 2021. The research stages started from the preparation of explant source plants, sterilization of explants and tools, manufacture of stock solutions and planting media, subculture, and analysis of the costs needed to provide superior sugarcane seeds. The results showed that the basal swelling of explants, increasing the number of shoots, root elongation, and the method of providing superior sugarcane seeds from apical shoot explants in vitro were relatively more competitive with the price of plantlets per culture tube contained 10 clones costly were Rp. 20,328, compared with conventional propagation methods. with the price of sugar cane seedlings material were Rp 1,603/stem.</em></p>

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“…Nepenthes can be propagated conventionally using seeds and stem cuttings or nonconventional propagation using tissue culture techniques (Devi et al, 2013;Dinarti et al, 2010;Rosmaina and Aryani, 2015;Sukamto, 2011;Nuryadin, 2018). However, Nepenthes is a dioecious plant whose insect such as Diptera and Hymenoptera assists the pollination, or sometimes aided by the wind, and many pitchers have offspring from crosses of several species and the resulting impurity seed (Cheek and Jebb, 2001).…”

Section: Nepenthes Reinwardtiana Miq) Through Callus Inductionmentioning

confidence: 99%

PROPAGATION OF PITCHER PLANTS (Nepenthes gracilis KORTH. AND Nepenthes reinwardtiana MIQ.) THROUGH CALLUS INDUCTION

Novitasari

Isnaini²

2021

Agric

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In vitro propagation of pitcher plants is still limited only using seeds, while the other in vitro methods using leaf explants such as embryogenesis, organogenesis, and callus culture has not been widely reported. The research aims to study the growth response of leaf explants in two species of pitcher plants (Nepenthes gracilis and Nepenthes reinwardtiana), which formed callus in several treatment media combinations. Leaf pieces were taken from a 4-month pitcher plant culture were grown on the treatment media, namely modified media of Murashige & Skoog with a half concentration (½ MS) added 2.4-Dichlorophenoxoxyacetate (2.4-D) with a concentration of 0, 0.5, 1, 1.5, and 2 mg L-1 and kinetin (0.5 mg L-1). The treatment was then stored in a dark environment to induce callus formation. The observation for 12 weeks showed that the time of callus formation in two species of pitcher plant observed was not significantly different. Both species of pitcher plants begin to form callus in the fourth week after treatment. None of the leaf explants were planted on the control medium without hormones formed callus. The best medium for callus induction in N. gracilis is ½ MS medium added with 2 mg L-1 2,4-D and 0.5 mg L-1 kinetin, with callus morphology brownish-white with friable texture. In comparison, the optimum callus media from leaf explant of N. reinwardtiana has not been obtained yet. Thus further research is still needed.

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OPTIMASI NAA DAN BAP TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TUNAS MIKRO TANAMAN KANTONG SEMAR (Nepenthes mirabilis) SECARA IN VITRO

Cited by 6 publications

References 0 publications

Untitled

Untitled

POTENSI DAN ANALISA BIAYA PENYEDIAAN BIBIT TEBU (Saccharum officinarum L.) UNGGUL SECARA IN VITRO DI PT. GUNUNG MADU PLANTATIONS

PROPAGATION OF PITCHER PLANTS (Nepenthes gracilis KORTH. AND Nepenthes reinwardtiana MIQ.) THROUGH CALLUS INDUCTION

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