Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics

Abstract

BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells' (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, a-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37°C), cell density (1 9 10 7 cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4°C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. Keywords Cell transporting Á Medium Á Cell density Á Amniotic fluid stem cell 1 Introduction Cell therapy using stem cells is considered as promising approach to cure degenerative diseases, cancer, damaged tissues, or any disease for which there are very limited Na-Hee Yu and So Young Chun contributed equally to this work.

In Vivo Safety and Regeneration of Long-Term Transported Amniotic Fluid Stem Cells for Renal Regeneration

“…Compared with the PLGA group, the values for both cell groups were significantly lower. Thus, the functional analysis confirmed that the Cells transported under conditions established in an in vitro experiment [3] showed recovery of total kidney volume, regeneration of glomerular/renal tubules, and low CD4/CD8 positive T cell infiltration. In addition, migration to distant organs and tumorigenicity were not observed over 40 weeks.…”

“…To minimize these problems, in a previous study we optimized the cell transport conditions [3] through an in vitro analysis of the medium, temperature, cell density, and container type. For AFSCs, we suggested an appropriate cell suspension medium comprising high-glucose Dulbecco's modified minimal Eagle's medium (DMEM), a transport temperature of 4 °C, a cell density of 1 9 10 7 / 2 mL, and a disposable syringe as the container.…”

“…For AFSCs, we suggested an appropriate cell suspension medium comprising high-glucose Dulbecco's modified minimal Eagle's medium (DMEM), a transport temperature of 4 °C, a cell density of 1 9 10 7 / 2 mL, and a disposable syringe as the container. Cells transported under these conditions maintained more than 80% survival and stem cell characteristics for 12 h [3]. These cells would be ready to use in the clinical field without additional treatment, such as removal of toxic cryoprotectants, and complex cell thawing steps to prevent osmotic shock.…”

The Role of Insulin-Like Growth Factor-2 on the Cellular Viability and Differentiation to the Osteogenic Lineage and Mineralization of Stem Cells Cultured on Deproteinized Bovine Bone Mineral

“…It was shown that IGF-2 promoted the adipogenic differentiation of stem cells at 100 ng/mL concentration [26]. Likewise, the net effects or optimal dosage may differ between the studies due to culturing conditions, type, and passages of cells [27]. Moreover, IGF-2 may have additional functions.…”

Fabrication of three-lobed magnetic microrobots for cell transportation

“…8 The cell-based therapies require the transportation of cells to the targeted locations for transplantation. 9 This transportation and delivery need to be precise, and a failed cell delivery could cause a serious immune response. 10 Microrobots are very suitable candidates to perform this crucial step for delivering cells accurately and noninvasively.…”