Optimal Sex Pheromone Composition for Monitoring Spodoptera exigua (Lepidoptera: Noctuidae) in Korea

Jung, Chung Ryul; Park, Young Jin; Boo, Kyung Saeng

doi:10.1016/s1226-8615(08)60183-1

Cited by 13 publications

(10 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to pheromone components, SfruOR13 also responds to pheromone analog Z9,E12-14:OAc, and SfruOR16 to pheromone analog Z9-14:OH. These two pheromone analogs are actually the sex pheromone components of S. exigua, and were used for male trapping in a ratio of 9:1 in the field [63][64][65].Z9,E12-14:OAc is also a minor sex pheromone component in another Spodoptera species S. litura [66]. In its native range in the Americas, the FAW share a similar geographic distribution with S. exigua [67]; thus, SfruOR13 and SfruOR16 are possibly involved in interspecific recognition between these two Spodoptera species.…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda)

Guo

Liu

et al. 2020

Insects

View full text Add to dashboard Cite

Pheromone receptors (PRs) found in the antennae of male moths play a vital role in the recognition of sex pheromones released by females. The fall armyworm (FAW), Spodoptera frugiperda, is a notorious invasive pest, but its PRs have not been reported. In this report, six candidate PRs (SfruOR6,11,13,16, 56 and 62) suggested by phylogenetic analysis were cloned, and their tissue-sex expression profiles were determined by quantitative real-time PCR (qPCR). All six genes except for SfruOR6 were highly and specifically expressed in the antennae, with SfruOR6, 13 and 62 being male-specific, while the other three (SfruOR11, 16 and 56) were male biased, suggesting their roles in sex pheromone perception. A functional analysis by the Xenopus oocyte system further demonstrated that SfruOR13 was highly sensitive to the major sex pheromone component Z9-14:OAc and the pheromone analog Z9,E12-14:OAc, but less sensitive to the minor pheromone component Z9-12:OAc; SfruOR16 responded weakly to pheromone component Z9-14:OAc, but strongly to pheromone analog Z9-14:OH; the other four candidate PRs did not respond to any of the four pheromone components and four pheromone analogs. This study contributes to clarifying the pheromone perception in the FAW, and provides potential gene targets for developing OR-based pest control techniques.

show abstract

Section: Discussionmentioning

confidence: 99%

Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda)

Guo

Liu

et al. 2020

Insects

View full text Add to dashboard Cite

show abstract

“…Three main components (Z9E12-14:Ac, Z9-14:OH, and Z9-14:Ac) have also been detected in Korean local populations of S . exigua , in which Z9E12-14:Ac can evoke more male antennal responses than others [ 7 ]. Thus, Z9E12-14:Ac is a major component of S .…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9 mutagenesis against sex pheromone biosynthesis leads to loss of female attractiveness in Spodoptera exigua, an insect pestt

et al. 2021

View full text Add to dashboard Cite

Virgin female moths are known to release sex pheromones to attract conspecific males. Accurate sex pheromones are required for their chemical communication. Sex pheromones of Spodoptera exigua, a lepidopteran insect, contain unsaturated fatty acid derivatives having a double bond at the 12th carbon position. A desaturase of S. exigua (SexiDES5) was proposed to have dual functions by forming double bonds at the 11th and 12th carbons to synthesize Z9,E12-tetradecedienoic acid, which could be acetylated to be a main sex pheromone component Z9,E12-tetradecenoic acetate (Z9E12-14:Ac). A deletion of SexiDES5 using CRISPR/Cas9 was generated and inbred to obtain homozygotes. Mutant females could not produce Z9E12-14:Ac along with Z9-14:Ac and Z11-14:Ac. Subsequently, pheromone extract of mutant females did not induce a sensory signal in male antennae. They failed to induce male mating behavior including hair pencil erection and orientation. In the field, these mutant females did not attract any males while control females attracted males. These results indicate that SexiDES5 can catalyze the desaturation at the 11th and 12th positions to produce sex pheromone components in S. exigua. This study also suggests an application of the genome editing technology to insect pest control by generating non-attractive female moths.

show abstract

“…Furthermore, the two species have a common sex pheromone component Z9,E12-14:OAc, while each species also uses some specific components. In S. exigua , several pheromone components, Z9,E12-14:OAc, Z9-14:OH, Z11-16:OAc, Z9-14:OAc, and Z9,E12-14:OH, were identified successively in the female pheromone glands of different populations (Persoons et al, 1981; Tumlinson et al, 1981, 1990; Mochizuki et al, 1993; Mitchell and Tumlinson, 1994; Dong and Du, 2002), with the former three components at the ratio of 7:3:1 attracting the most number of males in east Asia (Jung et al, 2003; Zhao et al, 2018). Different from S. exigua , the female S. litura showed highest attraction for males with Z9,E11-14:OAc and Z9,E12-14:OAc in a ratio of 9:1 (Tamaki et al, 1973).…”

Section: Introductionmentioning

confidence: 99%

Two Sympatric Spodoptera Species Could Mutually Recognize Sex Pheromone Components for Behavioral Isolation

et al. 2019

View full text Add to dashboard Cite

Spodoptera exigua and S. litura are two sympatric species in China and many other countries. Both moths employ a multiple component sex pheromone blend, including a common component Z9,E12-14:OAc, and two specific components Z9-14:OH and Z11-16:OAc for S. exigua, and one specific component Z9,E11-14:OAc for S. litura. For the two species, it has been well documented that males are able to recognize and behaviorally attracted by their species-specific sex pheromone, which functions as a means of reproductive isolation, but whether males could mutually recognize pheromone components of its sympatric species is unknown. In the present study, the electroantennogram (EAG) and field evaluation were conducted to address this topic. The EAG recordings revealed that males of each species could significantly respond to specific components of its sympatric species, although the response values were lower than that to its own major component. In field tests, the specific components Z9-14:OH and Z11-16:OAc of S. exigua strongly inhibited the male catches of S. litura to its conspecific sex pheromone, while specific component Z9,E11-14:OAc of S. litura significantly reduced the male catches of S. exigua to its sex pheromone. Furthermore, the combined lure of the two species completely inhibited male catches of S. litura, and significantly decreased the male catches of S. exigua, compared to the species-specific lure alone. The results demonstrated that males of the two sibling species could perceive the specific components of its counterpart, suggesting that mutual recognition of pheromone components may function to strengthen the behavioral isolation between the two species. Our study has added new knowledge to the reproductive isolation via sex pheromone communication system in sympatric moth species, and provided a base for designing of mating disruption tactics targeting multispecies by using insect sex pheromones.

show abstract

Optimal Sex Pheromone Composition for Monitoring Spodoptera exigua (Lepidoptera: Noctuidae) in Korea

Cited by 13 publications

References 12 publications

Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda)

Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda)

CRISPR/Cas9 mutagenesis against sex pheromone biosynthesis leads to loss of female attractiveness in Spodoptera exigua, an insect pestt

Two Sympatric Spodoptera Species Could Mutually Recognize Sex Pheromone Components for Behavioral Isolation

Contact Info

Product

Resources

About