Optimal Recovery of DNA for Polymerase Chain Reaction–Based Assays from Fine Needle Aspirates

Thyroid fine-needle aspiration samples inadequate for reverse transcriptase-polymerase chain reaction analysis

“…Mitteldorf et al have also demonstrated a correlation between the cellularity of FNAB samples and their adequacy for RT-PCR analysis. 52 Nevertheless, among 30 samples in which leftover material was analyzed for RT-PCR and which were inadequate for RT-PCR analysis and adequate for cytologic diagnosis, there were 8 samples in which many follicular cells were present. Takano et al offered a possible explanation for such results, suggesting that occasionally when a large volume of blood or cystic fluid is aspired, the majority of follicular cells in the aspirate can be washed out onto the slide glass with no or only few follicular cells remaining in the leftover material.…”

Local microstructure characterization of rare earth‐doped PMMA with low‐ion content by fluorescence EXAFS

“…A decrease in the number of follicular cells present in the sample increases the chance that the number of follicular cells present in the leftover material will be below the threshold for RT‐PCR. Mitteldorf et al have also demonstrated a correlation between the cellularity of FNAB samples and their adequacy for RT‐PCR analysis 52. Nevertheless, among 30 samples in which leftover material was analyzed for RT‐PCR and which were inadequate for RT‐PCR analysis and adequate for cytologic diagnosis, there were 8 samples in which many follicular cells were present.…”

Massively parallel DNA sequencing from routinely processed cytological smears

“…Examples includes 1) monoclonal antibodies (trastuzumab and cetuximab) in human epidermal growth factor receptor 2-positive breast cancer and wild-type KRAS colorectal cancer; 2) tyrosine kinase inhibitors (TKIs) such as imatinib, gefitinib, erlotinib, and crizotinib in chronic myeloid leukemia, gastrointestinal stromal tumors, and non-small cell lung cancers; and 3) intracellular agents (vemurafenib and olaparib) in metastatic malignant melanoma and ovarian, breast, and prostate cancers. 19 As shown in other studies, DNA extracted from previously stained cytological slides can be amplified with PCR [21][22][23][24][25] or can be used for conventional comparative genomic hybridization. 26 More recently, several authors have reported successful genetic analyses with qPCR and DNA targeted sequencing of dozens of hotspot mutations of interest.…”