Wrist-worn accelerometers can increase compliance with wearing accelerometers, however, several large scale studies continue to use hip-worn accelerometers and it is unclear how comparable data is from the two sites. The study aims were: to investigate agreement between wrist-and hip-worn accelerometers and to determine the validity of Johansson et al cut-points for wrist worn accelerometers in preschool children.A sample of 32 preschool children (21 boys, 4.2 (0.5) years, BMI 16.6 (1.1)) were videoed wearing GT3X+ accelerometers on their wrist and hip while they engaged in 1 hour of free-play in their nursery.Children's activity were coded using, the Children's Activity Rating Scale (CARS): with CARS, level 1 'sedentary' and levels 2 to 5 were classified as time spent in TPA. Accelerometry data were processed using Johansson et al cut-points for the wrist data and Evenson et al cut-points for the hip data, into time spent in different intensities of PA. The mean counts per minute (cpm) from the hip and wrist were compared.There was a strong correlation between the hip and wrist cpm (r=0.81, p<0.01) and total count data (r=0.83 p<0.01), however there was a large systematic bias with wide limits of agreement. Good agreement (mean difference (LOA) 1.1 (-9.9, 12.1) was found between the CARS estimate of TPA (29.