Optimal parameters for cinnabarin synthesis byPycnoporus sanguineus

Smânia, Elza F. A.; Smânia, Artur; Loguercio-Leite, Clarice; Gil, Marcio Luiz

doi:10.1002/(sici)1097-4660(199709)70:1<57::aid-jctb650>3.0.co;2-4

Cited by 21 publications

(7 citation statements)

References 9 publications

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“…A significantly higher yield was produced in lighted condition with 168.47 mg/30 ml compared to dark (87.6 mg/30 ml). This result is in congruence with the findings of Smania et al (1997) wherein P. sanguineus showed higher biomass yield under lighted condition. It was reported that light has a positive impact on the fruiting body development of king oysters (Zadrazil, 1978).…”

Section: Influence Of Illuminationsupporting

confidence: 91%

“…Moreover, Dulay et al (2021a) found out that room temperature favors luxuriant mycelial growth of various Lentinus species. Contrastingly, Smania et al (1997), Alberti et al (2021), and Lee et al (2004 observed that the optimum mycelial growth and antibiotic production of P. sanguineus, Oudemansiella canarii, and Grifola frondosa were favored under 25°C. According to Lin (2004), the temperature preferences of fungi can be used to classify them into three groups; these are temperate, tropical, or semitemperate.…”

Section: Influence Of Temperaturementioning

confidence: 97%

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Optimal liquid culture conditions and bioactivity of Pycnoporus sanguineus

Aguilar¹,

Dulay²,

Kalaw³

et al. 2023

J App Pharm Sci

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Pycnoporus sanguineus is a species of mushroom in the Philippines with promising nutritional and pharmacological potentials. In this study, the optimum nutritional and physical growth preferences of P. sanguineus in submerged conditions were established and the biological activities of ethanol extract of its mycelia were determined. The highest yield (100.13 mg/30ml) was produced in rice bran broth with a wide range of pH concentration. Biomass production was favored under 28°C, in lighted and agitated conditions with 147.20 mg/30 ml, 168.47 mg/30 ml, and 413.30 mg/30 ml, respectively. The extract inhibited the growth of Staphylococcus aureus while no antibacterial activity was observed in Escherichia coli. It was found toxic to brine shrimp with an LC 50 value of 190.55 µg/ml. Exposure of zebrafish embryos to the extract caused delayed growth resulting in a lower hatching rate. Growth retardation was the most noticeable teratogenic effect of the extract after 48 hours and high mortality rates were observed in high extract concentrations. This study suggests that the optimum biomass yield of P. sanguineus can be achieved when incubated under the most favorable conditions. Mycelia of P. sanguineus could be a valuable resource of antibacterial and cytotoxic compounds.

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Section: Influence Of Illuminationsupporting

confidence: 91%

Section: Influence Of Temperaturementioning

confidence: 97%

Optimal liquid culture conditions and bioactivity of Pycnoporus sanguineus

Aguilar¹,

Dulay²,

Kalaw³

et al. 2023

J App Pharm Sci

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“…Among the tested wastes, the wood chip was found to be the best substrate not only giving a maximum pigment yield but also greatly promoting the growth of strain SYBC-L7. In the literature, most Pycnoporus species have been isolated and used as enzyme producers ( Tellez-Tellez et al, 2016 ), but few of them were used to produce pigment, which was shown in ( Supplementary Table S1 ; Eggert, 1997 ; Smania et al, 1997 ; Cruz-Munoz et al, 2015 ; Sutthisa and Sanoamuang, 2017 ; Zhang et al, 2019 ). However, our study is the first report, to our knowledge, on using agro-industrial waste, especially wood chips as a culture substrate for pigment production by Pycnoporus sanguineus SYBC-L7.…”

Section: Resultsmentioning

confidence: 99%

Pigment production by a newly isolated strain Pycnoporus sanguineus SYBC-L7 in solid-state fermentation

Meng

Shao²,

Luo³

et al. 2022

Front. Microbiol.

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Natural pigments are playing important roles in our daily lives. They not only make products colorful but also provide various health benefits for humans. In addition, Pycnoporus genus, listed as food- and cosmetic-grade microorganism, is one of the promising organisms for developing natural pigments. In this study, a new fungal strain with high efficiency in producing intense orange pigments was isolated and identified as Pycnoporus sanguineus SYBC-L7. Different agro-industrial wastes were applied to evaluate the growth and pigment production of strain SYBC-L7. SYBC-L7 can grow rapidly and effectively produce pigments using wood chips as substrate in solid-state fermentation (SSF). Culture conditions were also optimized for value-added pigments production and the optimum production conditions were glucose as carbon source, ammonium tartrate as nitrogen source, initial pH 6.0, and relative humidity of 65%. Pigment components, cinnabarinic acid, tramesanguin, and 2-amino-9-formylphenoxazone-1-carbonic acid were confirmed by liquid chromatography–mass spectrometry. Meanwhile, an agar plate diffusion assay was performed to evaluate the antimicrobial activity of the pigment. These pigments showed more significant inhibition of Gram-positive than Gram-negative bacteria. The results showed that Pycnoporus sanguineus SYBC-L7 was able to cost-effectively produce intense natural orange pigments with antibacterial activity in SSF, which is the basis of their large-scale production and application.

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“…Subsequently, the flask was placed under continuous shaking at 1000 rpm for 24 h. The ethyl acetate fraction was filtered using Whatman No. 4 paper and subsequently concentrated on a Büchi rotary evaporator (461) at 75 • C [20]. Finally, the crude extract was weighed and stored in amber bottles.…”

Section: Production and Synthesis Of Cinnabarin From Pycnoporus Sangu...mentioning

confidence: 99%

“…Finally, cinnabarin synthesis was indirectly determined by the agar diffusion method, where in cinnabarin concentration in each treatment was estimated by the formula Y = AX + B; where (Y) corresponds to the diameter of the zone of inhibition (mm), (AX) is equivalent to the logarithm of the amount of cinnabarin concentrate (InU/g) in ethyl acetate extract that produced an inhibition equivalent to 1 mg of cinnabarin [20] and (B) represents different treatments. The experiments were carried out in triplicate.…”

Section: Purification Of Cinnabarinmentioning

confidence: 99%

In Vitro Antimicrobial Activity of Cinnabarin on Xanthomonas campestris Isolated from Bean Crops of Puebla, Mexico

et al. 2021

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Phaseolus vulgaris is the most legume cultivated in the world; in Mexico, it is considered the second most important crop after corn. The aim of this research was to determine the characteristics of Xanthomonas campestris strain “Xcf1-APJR” isolated from the leaves of bean crops, and determine the antimicrobial activity of cinnabarin on this strain. Bacterial cultures were obtained from leaves with necrotic leaf spot symptoms of bean plant variety “Flor de Mayo M38” in Puebla, Mexico. The antimicrobial activity of cinnabarin was tested at 7, 14 and 21 days on X. campestris pv. campestris. The Xcf1-APJR strain showed 100% identity with X. campestris pv. campestris as a causal agent of necrotic leaf spot. Treatment with a potato dextrose medium with a dehydrated sugar cane (PDA+C) showed a higher orange pigmentation than the other treatments after 7, 14 and 21 days of incubation and a higher concentration of cinnabarin (54.33 InU/g) with in vitro antimicrobial activity against X. campestris pv. campestris.

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Optimal parameters for cinnabarin synthesis byPycnoporus sanguineus

Cited by 21 publications

References 9 publications

Optimal liquid culture conditions and bioactivity of Pycnoporus sanguineus

Optimal liquid culture conditions and bioactivity of Pycnoporus sanguineus

Pigment production by a newly isolated strain Pycnoporus sanguineus SYBC-L7 in solid-state fermentation

In Vitro Antimicrobial Activity of Cinnabarin on Xanthomonas campestris Isolated from Bean Crops of Puebla, Mexico

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