Optimal neural differentiation and extension of hybrid neuroblastoma cells (NDC) for nerve-target evaluations using a multifactorial approach

“…Griffith, who has used NDC as a source of neural progeny to evaluate biocompatibility on different biomaterials [52,53]. For culturing NDC, xenografts were soaked in DMEM media (Sigma-Aldrich) supplemented with 10% (v/v) FBS (Life Technologies Corporation) for seven days.…”

Section: Hybrid Neuroblastoma Cells (Ndc)-ndc Were Kindly Provided Bymentioning

confidence: 99%

Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts

et al. 2019

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To address the shortcomings associated with corneal transplants, substantial efforts have been focused on developing new modalities such as xenotransplantion. Xenogeneic corneas are anatomically and biomechanically similar to the human cornea, yet their applications require prior decellularization to remove the antigenic components to avoid rejection. In the context of bringing decellularized corneas into clinical use, sterilization is a crucial step that determines the success of

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“…After E‐beam irradiation of 8.0 mm PMMA discs, they were placed in a 48‐ well cell culture plates and washed with sterile phosphate buffered saline (PBS). Next, HCF (10000) or HCEp (5000) were seeded on each disc suspended in 20 µL of respective media (i.e., HCF: low glucose DMEM media (Gibco; Gaithersburg, MD) supplemented 1mM of L‐ascorbic acid 2‐phosphate (Sigma‐Aldrich, St. Louis, MO), 1x Insulin‐Transferrin‐Selenium Supplement (ITS; Sigma‐Aldrich), 1% Glutamax (Gibco), 1x penicillin/streptomycin (Gibco), 1g/L of D‐glucose (Sigma‐Aldrich), and 2.5 g/L of D‐mannitol (Sigma‐Aldrich); and HCEp: serum‐free medium (KSFM) supplemented with 50 µg/ml bovine pituitary extract and 5 ng/ml epidermal growth factor (Gibco)) and allowed to adhere for 30 min prior to addition of 500 µL of extra media, [ 10b,33 ] followed by incubation at 37°C and 5% CO 2 for up to 7 days. The respective culture media was changed every other day.…”

Section: Methodsmentioning

confidence: 99%

Electron Beam Sterilization of Poly(Methyl Methacrylate)—Physicochemical and Biological Aspects

Sharifi

Islam

Sharifi

et al. 2021

Macromolecular Bioscience

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Electron beam (E‐beam) irradiation is an attractive and efficient method for sterilizing clinically implantable medical devices made of natural and/or synthetic materials such as poly(methyl methacrylate) (PMMA). As ionizing irradiation can affect the physicochemical properties of PMMA, understanding the consequences of E‐beam sterilization on the intrinsic properties of PMMA is vital for clinical implementation. A detailed assessment of the chemical, optical, mechanical, morphological, and biological properties of medical‐grade PMMA after E‐beam sterilization at 25 and 50 kiloGray (kGy) is reported. Fourier transform infrared spectroscopy, thermogravimetric analysis, and differential scanning calorimetry studies indicate that E‐beam irradiation has minimal effect on the chemical properties of the PMMA at these doses. While 25 kGy irradiation does not alter the mechanical and optical properties of the PMMA, 50 kGy reduces the flexural strength and transparency by 10% and 2%, respectively. Atomic force microscopy demonstrates that E‐beam irradiation reduces the surface roughness of PMMA in a dose dependent manner. Live‐Dead, AlamarBlue, immunocytochemistry, and complement activation studies show that E‐beam irradiation up to 50 kGy has no adverse effect on the biocompatibility of the PMMA. These findings suggest that E‐beam irradiation at 25 kGy may be a safe and efficient alternative for PMMA sterilization.

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Optimal neural differentiation and extension of hybrid neuroblastoma cells (NDC) for nerve-target evaluations using a multifactorial approach

Cited by 7 publications

References 68 publications

Immortalized Dorsal Root Ganglion Neuron Cell Lines

Immortalized Dorsal Root Ganglion Neuron Cell Lines

Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts

Electron Beam Sterilization of Poly(Methyl Methacrylate)—Physicochemical and Biological Aspects

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