Optimal methods for fluorescence and diffuse reflectance measurements of tissue biopsy samples

Palmer, Gregory M.; Marshek, Crystal L.; Vrotsos, Kristin M.; Ramanujam, Nirmala

doi:10.1002/lsm.10026

Cited by 61 publications

(51 citation statements)

References 22 publications

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“…AF in colonic tissue from intracellular fluorophores such as NAD(P)H has been reported to decay ex vivo over a time scale of 118 minutes due to tissue deoxygenation, while the AF from collagen and flavins remained relatively constant [33]. In addition, similar AF intensity and spectral line shapes to that of in vivo tissue have been reported for freshly excised tissue in a hamster cheek pouch model [71]. In a study of ischaemic mouse skin by Palero et al [72] using spectrally resolved multiphoton microscopy, the change in fluorescence intensity was reported to increase by 71% compared to baseline over approximately one hour and the change in peak emission wavelength shifted from 456 nm to 450 nm after 20 minutes.…”

Section: Discussionmentioning

confidence: 83%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

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Abstract:We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime −570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens. References and links1. R. Lambert, H. Saito, and Y. Saito, "High-resolution endoscopy and early gastrointestinal cancer...dawn in the East," Endoscopy 39(3), 232-237 (2007

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Section: Discussionmentioning

confidence: 83%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

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“…A detailed description of the instrument is provided elsewhere (17). A fiber-optic probe can be coupled to the sample compartment (F3000, JY Horbia Inc., NJ), thus enabling optical spectroscopic measurements to be made remotely from the hamster cheek pouch.…”

Section: Instrument Descriptionmentioning

confidence: 99%

Investigation of fiber‐optic probe designs for optical spectroscopic diagnosis of epithelial pre‐cancers

et al. 2004

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Background and Objective-The first objective of this study was to evaluate the performance of fluorescence spectroscopy for diagnosing pre-cancers in stratified squamous epithelial tissues in vivo using two different probe geometries with (1) overlapping vs. (2) non-overlapping illumination and collection areas on the tissue surface. Probe (1) and probe (2) are preferentially sensitive to the fluorescence originating from the tissue surface and sub-surface tissue depths, respectively. The second objective was to design a novel, angled illumination fiber-optic probe to maximally exploit the depth-dependent fluorescence properties of epithelial tissues.

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“…Several studies have demonstrated that fluorescence spectroscopy can be used for discrimination of normal and malignant oral tissues. [6][7][8][9][10][11][12][13][14][15][16][17][18][19] We have developed a Raman spectroscopy method with specificity and sensitivity better than 95%, for discrimination of normal and malignant tissues in oral cancers. 20,21 Recent Raman microspectroscopy studies of formalin-fixed oral tissues have validated the use of these samples in optical pathology.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of normal, inflammatory, premalignant, and malignant oral tissue: A Raman spectroscopy study

et al. 2005

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Optical spectroscopy methods are fast emerging as potential alternatives for early diagnosis of cancer. A Raman spectroscopy method for discrimination of normal and malignant oral tissues has been developed by us earlier. It is necessary to evaluate and establish the validity of the approach before it can be routinely used. In the present study, our Raman spectroscopy investigations are extended further to evaluate the efficacy of the technique to discriminate between normal, inflammatory, premalignant, and malignant conditions in oral tissue. Spectral profiles of normal, malignant, premalignant, and inflammatory conditions show pronounced differences between one another. Spectra of normal tissues can be attributed mainly to lipids whereas pathological tissue spectra are dominated by proteins. Principal components analysis (PCA) of the spectral data sets belonging to the four different categories showed that scores of factors differentiated between

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Optimal methods for fluorescence and diffuse reflectance measurements of tissue biopsy samples

Cited by 61 publications

References 22 publications

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Investigation of fiber‐optic probe designs for optical spectroscopic diagnosis of epithelial pre‐cancers

Discrimination of normal, inflammatory, premalignant, and malignant oral tissue: A Raman spectroscopy study

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