BACKGROUNDNowadays, organic farming has become a feasible approach for the production of high‐quality fruits. To evaluate the response of fruit quality and mineral nutrition contents of Hayward Kiwifruit affected by different organic and inorganic fertilizers, this study was conducted in Citrus and Subtropical Fruits Research Center, Iran in 2017–2021 as a randomized block design with three replications. The studied treatments were organic fertilizers (cow, vermicompost, and Azolla) and chemical fertilizers. After four years of fertilization, the fruit's nutritional elements content and some fruit bioactive compounds were evaluated after three months of cold storage and then analyzed by the principal component analysis (PCA) method.RESULTSThe use of organic amendments boosted the calcium, phosphorus, potassium, and iron content of the kiwifruits when compared to chemical fertilizers. The highest fruit potassium and phosphorus content were recorded in the cow manure treatment. The lowest amount of nitrate and the highest calcium, zinc, copper, and manganese accumulation were recorded in the fruits treated with vermicompost. In addition to mineral nutrients, the dry matter, total soluble solids, total phenolic, and antioxidant capacity of kiwifruit were improved by the application of vermicompost amendment compared with the other fertilizer sources. However, the highest fruit vitamin C and total soluble carbohydrates were measured in the cow manure treatment. The PCA results of the fruit quality indices indicated that fertilization treatments were ranked as vermicompost (1.88) > cow manure (1.63) = chemical (1.60) > Azolla (1.54).CONCLUSIONIt is concluded that the application of 40 kg vermicompost or 40 kg cow manure in the next rank in Hayward kiwifruit orchards in March (growth stage beginning of bud swelling) may be a more suitable approach for improving the nutritional quality of the fruit.This article is protected by copyright. All rights reserved.