Optimal management of extreme oligozoospermia by an appropriate cryopreservation programme

Koscinski, Isabelle; Wittemer, C.; Lefebvre‐Khalil, V.; Marcelli, F.; Défossez, A.; Rigot, Jean-Marc

doi:10.1093/humrep/dem190

Cited by 33 publications

(24 citation statements)

References 32 publications

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“…The results of ICSI for the whole cohort of patients are similar to those in the literature [7]. When we compare the results according to the sperm count, we find significant differences in the cleavage rate and the number of frozen embryos while no difference is observed in age, neither for women nor for men.…”

Section: Discussionsupporting

confidence: 83%

“…In other centers, synchronous ICSI-TESE is not possible and they have developed a politic of extended search in repeated ejaculates according to Koscinski et al [7] combined to microfreezing before the ovarian stimulation has started. This option of “security straw” freezing avoids not only cancellation of the ICSI but also a premature TESE [7, 8].…”

Section: Introductionmentioning

confidence: 99%

“…This option of “security straw” freezing avoids not only cancellation of the ICSI but also a premature TESE [7, 8]. Extended to the patients who have very low sperm count, this micro-freezing of security straws can also prevent further azoospermia of these patients.…”

Section: Introductionmentioning

confidence: 99%

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In cryptozoospermia or severe oligozoospermia is sperm freezing useful?

Bessonnat

Brouillet

Sintzel

et al. 2014

Basic Clin. Androl.

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BackgroundIntracytoplasmic Sperm Injection (ICSI) is an Assisted Reproduction Technique (ART) which offers the chance to conceive to patients presenting very low sperm counts (cryptozoospermia/severe oligozoospermia). Sperm freezing before the oocyte pick-up, can prevent from a lack of spermatozoa on the day of the ICSI. It can avoid the cancellation of the ICSI or the use of TESE (Testicular sperm extraction). The objective of this study was to analyse the practice of sperm freezing for these patients in our center over 8 years and the rate of use of these frozen sperms. We also compared the outcome of ICSIs with frozen versus ejaculated sperm.Material and methodsWe performed a retrospective epidemiological study between 2004 and 2011. We recruited all the patients having a sperm count below 1 Million/mL and who were waiting for their first ICSI attempt.Results169 patients were recruited: 84 cryopreserved their sperm before the ICSI (secured ICSI) while 85 did not (non-secured ICSI). Both groups were split in cryptozoospermia (<103 spermatozoa/ml): 19 and 17 patients respectively, very severe oligozoospermia (103–105/ml): 37 and 13 patients, and severe oligozoospermia (105–106/ml): 28 and 55 patients. The part of secured ICSI significantly increased from 29% during 2004–2007 to 74% during 2008–2011(p = 0.0029) and the frozen sperm was used in 5.9% of the cases. Median age was significantly higher in the non secured ICSI group (33.57 vs 35.52 for men, p = 0.0069 and 30.45 vs 32.26 for women, p = 0.025) but no significant difference was found in the outcome of the ICSI between frozen-thawed sperm and fresh ejaculated sperm.ConclusionSperm freezing before ICSI for severe oligozoospermic and cryptozoospermic patients significantly increased in our practice but the rate of use remain very low. This encourages to define more accurate criteria leading to sperm freezing.Electronic supplementary materialThe online version of this article (doi:10.1186/2051-4190-24-15) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In cryptozoospermia or severe oligozoospermia is sperm freezing useful?

Bessonnat

Brouillet

Sintzel

et al. 2014

Basic Clin. Androl.

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“…In addition, sperm cryopreservation is increasingly used in case of other disorders, such as autoimmune diseases and myelodysplastic syndromes requiring treatments that may affect reproductive functions. Moreover, sperm cryopreservation is offered to patients with severe oligospermia (or even cryptozoospermia) or ejaculatory disorders with the intent of using cryopreserved sperm in case no sperm are found in the ejaculates on the day of intracytoplasmic sperm injection (ICSI) (1) to have sperm available in the event of a decline in sperm count, which may occur in these patients (2). Cryopreservation of spermatozoa was introduced in the early 1960s and is performed with different procedures.…”

mentioning

confidence: 99%

Semen cryopreservation for men banking for oligospermia, cancers, and other pathologies: prediction of post-thaw outcome using basal semen quality

Degl'Innocenti

Filimberti

Magini

et al. 2013

Fertility and Sterility

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Objective: To evaluate post-thawing sperm parameters in a large series of men cryopreserving for different cancers and oligospermia. Design: Retrospective observational study. Setting: Semen cryopreservation laboratory. Patient(s): Six hundred twenty-three patients undergoing semen cryopreservation for cancer or oligospermia who discontinued banking. Intervention(s): None. Main Outcome Measure(s): Postcryopreservation sperm motility and viability. Result(s): In oligospermic men, recovery of motile sperm after cryopreservation was possible in only a few out of the 219 samples cryopreserved for this problem. Similarly, independent of the reason for which cryopreservation was required, if one basal semen parameter fell below the 5th percentile of the World Health Organization reference values, recovery of motile and viable spermatozoa after thawing was low. Among samples cryopreserved for cancer, those with testicular cancer showed the lowest basal semen quality and recovery after thawing. In cases of hematological cancers or other types of cancers, motility recovery was similar to that of non-cancerrelated samples. Receiver operating characteristic analyses demonstrate that basal progressive and total motility predict the recovery rate of motile sperm after thawing with high accuracy, sensibility and specificity. Conclusion(s): Our study demonstrates the ability of prefreeze semen parameters to predict cryosurvival in terms of sensitivity and precision. Using this information, the clinician could perform appropriate counseling about the future possibilities of fertility for the patient. (Fertil Steril Ò 2013;100:1555-63.

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“…When the initial sample is of very poor quality or with very small number of cells, small cell-number or single cell cryopreservation techniques may become useful. Starting in the late 1990's , reports on several single sperm cryopreservation techniques showed up in the scientific literature (Walmsley et al, 1998;Gil-Salom et al, 2000;Gvakharia & Adamson, 2001;Just et al, 2004;Herrler et al, 2006;Isaev et al, 2007;Koscinski et al, 2007;Woods et al, 2010). Using these various techniques, researchers reported a wide range of outcomes and recovery efficiency.…”

Section: Semen Freezingmentioning

confidence: 99%