Optimal isolation, culture, and in vitro propagation of spermatogonial stem cells in Huaixiang chicken
Fahar Ibtisham,
Shuyan Tang,
Yiping Song
et al.
Abstract:Spermatogonial stem cells (SSCs) comprise the foundation of spermatogenesis and hence have great potential for fertility preservation of rare or endangered species and the development of transgenic animals and birds. Yet, developing optimal conditions for the isolation, culture, and maintenance of SSCs in vitro remains challenging, especially for chicken. The objectives of this study were to (1) find the optimal age for SSC isolation in Huaixiang chicken, (2) develop efficient protocols for the isolation, (3) … Show more
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