Optimal imaging windows of indocyanine green‐assisted near‐infrared dental imaging with rat model and its comparison to X‐ray imaging

Li, Zhongqiang; Hartzler, Thomas; Ramos, Alexandra; Osborn, Michelle L.; Li, Yanping; Yao, Shaomian; Xu, Jian

doi:10.1002/jbio.201960232

Cited by 7 publications

(10 citation statements)

References 39 publications

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“…Although application of PB is a feasible procedure, detection of fine (neo) vasculature system is often not possible due to its relatively low sensitivity by optical wave length-light. Hence, visualization and in vivo imaging by various dye injection is becoming frequently reported [ 21 – 24 ]. Injected PB after the current procedure showed the successful ligation and enlargement of the main L.V.…”

Section: Resultsmentioning

confidence: 99%

Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model

et al. 2021

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Background Because of the high frequency of chronic edema formation in the current “aged” society, analyses and detailed observation of post-surgical edema are getting more required. Post-surgical examination of the dynamic vasculature including L.V. (Lymphatic Vasculature) to monitor edema formation has not been efficiently performed. Hence, procedures for investigating such vasculature are essential. By inserting transparent sheet into the cutaneous layer of mouse tails as a novel surgery model (theTailEdema bySilicone sheet mediatedTransparency protocol; TEST), the novel procedures are introduced and analyzed by series of histological analyses including video-based L.V. observation and 3D histological reconstruction of vasculatures in mouse tails. Results The dynamic generation of post-surgical main and fine (neo) L.V. connective structure during the edematous recovery process was visualized by series of studies with a novel surgery model. Snapshot images taken from live binocular image recording for TEST samples suggested the presence of main and elongating fine (neo) L.V. structure. After the ligation of L.V., the enlargement of main L.V. was confirmed. In the case of light sheet fluorescence microscopy (LSFM) observation, such L.V. connections were also suggested by using transparent 3D samples. Finally, the generation of neo blood vessels particularly in the region adjacent to the silicone sheet and the operated boundary region was suggested in 3D reconstruction images. However, direct detection of elongating fine (neo) L.V. was not suitable for analysis by such LSFM and 3D reconstruction procedures. Thus, such methods utilizing fixed tissues are appropriate for general observation for the operated region including of L.V. Conclusions The current surgical procedures and analysis on the post-surgical status are the first case to observe vasculatures in vivo with a transparent sheet. Systematic analyses including the FITC-dextran mediated snap shot images observation suggest the elongation of fine (neo) lymphatic vasculature. Post-surgical analyses including LSFM and 3D histological structural reconstruction, are suitable to reveal the fixed structures of blood and lymphatic vessels formation.

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Section: Resultsmentioning

confidence: 99%

Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model

et al. 2021

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“…15 Early detection plays an important role in the diagnosis of a crack, which could significantly limit further growth of the crack and save the tooth. 10,13 In our previous pilot studies, we demonstrated the feasibility of ICG-NIRF dental imaging based on both a rat model 40,41,44 and human extracted teeth. 42,43 These results showed the promise of using ICG-NIRF dental imaging for crack detection.…”

Section: Discussionmentioning

confidence: 99%

“…37,38 In our previous research we demonstrated the feasibility of indocyanine green (ICG) for dental imaging in the rat model and human extracted tooth. [39][40][41][42][43][44] We found that ICF-NIR fluorescent (ICG-NIRF) imaging could diagnose several typical diseases in human extracted teeth, such as caries lesions, decay, and insidious caries. 42,43 In particular, we compared ICG-NIRF dental imaging with X-ray imaging.…”

Section: Introductionmentioning

confidence: 99%

Detection and analysis of enamel cracks by ICG‐NIR fluorescence dental imaging

Holamoge

et al. 2020

Annals of the New York Academy of Sciences

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Cracked teeth are the third most common cause of tooth loss, but there is no reliable imaging tool for the diagnosis of cracks. Here, we demonstrate the feasibility of indocyanine green near‐infrared fluorescence (ICG‐NIRF) dental imaging for the detection of enamel cracks and enamel–dentin cracks in vitro in the first (ICG‐NIRF‐I, 700–950 nm) and second (ICG‐NIRF‐II, 950–1700 nm) imaging windows with transmission excitation light, and compared ICG‐NIRF with conventional NIR illumination‐II (NIRi‐II) and X‐ray imaging. Dentin cracks were detected by CT scan, while most enamel cracks, undetectable under X‐ray imaging, were clearly visible in NIR images. We found that ICG‐NIRF‐II detected cracks more effectively than NIRi‐II, and that light orientation is an important factor for crack detection: an angled exposure obtained better image contrast of cracks than parallel exposure, as it created a shadow under the crack. Crack depth could be evaluated from the crack shadow in ICG‐NIRF and NIRi‐II images; from this shadow we could determine crack depth and discriminate enamel–dentin cracks from craze lines. Cracks could be observed clearly from ICG‐NIRF images with 1‐min ICG tooth immersion, although longer ICG immersion produced images with greater contrast. Overall, our data show that ICG‐NIRF dental imaging is a useful tool for diagnosing cracked teeth at an early stage.

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“… 11 , 19 – 27 Our previous studies have successfully demonstrated ICG-NIR fluorescence (ICG-NIRF) dental imaging in both human-extracted tooth and rat models. 17 , 28 – 32 …”

Section: Introductionmentioning

confidence: 99%

“… 17 , 47 In our previous rat model studies, ICG-NIRF dental imaging was only performed in the short NIR wavelength. 29 – 32 Few studies have reported using ICG for dental imaging in the second NIR windows to our best knowledge.…”

Section: Introductionmentioning

confidence: 99%

Mouthwash as a non-invasive method of indocyanine green delivery for near-infrared fluorescence dental imaging

Zaid

et al. 2022

J. Biomed. Opt.

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. Significance: X-ray imaging serves as the mainstream imaging in dentistry, but it involves risk of ionizing radiation. Aim: This study presents the feasibility of indocyanine green-assisted near-infrared fluorescence (ICG-NIRF) dental imaging with 785-nm NIR laser in the first (ICG-NIRF-I: 700 to 1000 nm) and second (ICG-NIRF-II: 1000 to 1700 nm) NIR wavelengths. Approach: Sprague Dawley rats with different postnatal days were used as animal models. ICG, as a fluorescence agent, was delivered to dental structures by subcutaneous injection (SC) and oral administration (OA). Results: For SC method, erupted and unerupted molars could be observed from ICG-NIRF images at a short imaging time ( ). ICG-NIRF-II could achieve a better image contrast in unerupted molars at 24 h after ICG injection. The OA could serve as a non-invasive method for ICG delivery; it could also cause the glow-in-dark effect in unerupted molars. For erupted molars, OA can be considered as mouthwash and exhibits outstanding performance for delivery of ICG dye; erupted molar structures could be observed at a short imaging time ( ) and low ICG dose ( ). Conclusions: Overall, ICG-NIRF with mouthwash could perform in-vivo dental imaging in two NIR wavelengths at a short time and low ICG dose.

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Optimal imaging windows of indocyanine green‐assisted near‐infrared dental imaging with rat model and its comparison to X‐ray imaging

Cited by 7 publications

References 39 publications

Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model

Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model

Detection and analysis of enamel cracks by ICG‐NIR fluorescence dental imaging

Mouthwash as a non-invasive method of indocyanine green delivery for near-infrared fluorescence dental imaging

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