Optimal Feed Trajectories for Fedbatch Fermentation with Substrate Inhibition Kinetics

Ryde, Thomas Emil; Wahlgreen, Morten Ryberg; Nielsen, Marcus Krogh; Hørsholt, Steen; Jørgensen, Sten Bay; Jørgensen, John Bagterp

doi:10.1016/j.ifacol.2021.08.261

Cited by 5 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, our results show that there exists multiple feeding trajectories resulting in the same mAb production. Similar results has been obtained for fed-batch fermentation with Haldane growth kinetics (Ryde et al, 2021).…”

Section: Introductionsupporting

confidence: 87%

See 1 more Smart Citation

Dynamic Optimization for Monoclonal Antibody Production

Kaysfeld¹,

Kumar²,

Nielsen³

et al. 2023

Preprint

View full text Add to dashboard Cite

This paper presents a dynamic optimization numerical case study for Monoclonal Antibody (mAb) production. The fermentation is conducted in a continuous perfusion reactor. We represent the existing model in terms of a general modeling methodology well-suited for simulation and optimization. The model consists of six ordinary differential equations (ODEs) for the non-constant volume and the five components in the reactor. We extend the model with a glucose inhibition term to make the model feasible for optimization case studies. We formulate an optimization problem in terms of an optimal control problem (OCP) and consider four different setups for optimization. The optimization results show that optimal operation of the continuous perfusion reactor increases the mAb production with up to 52% compared to the base case. Additionally, our results show that multiple optimal feeding trajectories exists and that full glucose utilization can be forced without loss of mAb formation.

show abstract

Section: Introductionsupporting

confidence: 87%

“…We notice that forcing full glucose utilization does not effect the final production of mAb. This shows that there are multiple optimal solutions all resulting in the same mAb production (Ryde et al, 2021). As such, optimization setup (2) and (4) are preferred under the assumption that glucose has a cost.…”

Section: Optimizationmentioning

confidence: 99%