Optimal extraction, purification and antioxidant activity of total flavonoids from endophytic fungi of Conyza blinii H. Lév

Abstract

Background Flavonoids are widely used in the market because of their antibacterial, antiviral, and antioxidant activities. But the production speed of flavonoids is limited by the growth of plants. CBL9 (Chaetomium cruentum) is a flavonoid-producing endophytic fungi from Conyza blinii H. Lév, which has potential to produce flavonoids. Methods In this study, we isolated total flavonoids from endophytic fungus CBL9 of Conyza blinii H. Lév using macroporous resin D101. The process was optimized by response surface and the best extraction process was obtained. The antioxidant activities of total flavonoids were analyzed in vitro. Results It was found that the best parameters were 25 °C pH 2.80, 1.85 h, and the adsorption ratio reached (64.14 ± 0.04)%. A total of 60% ethanol was the best elution solvent. The elution ratio of total flavonoid reached to (81.54 ± 0.03)%, and the purity was 7.13%, which was increased by 14.55 times compared with the original fermentation broth. Moreover its purity could rise to 13.69% after precipitated by ethanol, which is very close to 14.10% prepared by ethyl acetate extraction. In the antioxidant research, the clearance ratio of L9F-M on DPPH, ABTS, •OH, •O2−, (96.44 ± 0.04)% and (75.33 ± 0.03)%, (73.79 ± 0.02)%, (31.14 ± 0.01)% at maximum mass concentration, was higher than L9F. Conclusion The result indicated using macroporous resin in the extraction of total flavonoid from endophytic fungus is better than organic solvents with higher extraction ratio, safety and lower cost. In vitro testing indicated that the flavonoid extracted by macroporous resin have good antioxidant activity, providing more evidence for the production of flavonoid by biological fermentation method.

Community composition, bacterial symbionts, antibacterial and antioxidant activities of honeybee-associated fungi

“…Notably, the crude extract of C. subaffine MFFC22 isolated from honeycomb has shown notable antioxidant activity. Chaetomium genus is already known to have stron g DPPH scavenging activity, such as C. nigricolor , C. globosum , C. cruentum [ 26 , 44 , 45 ]. Nevertheless, C. subaffine and its metabolites have not reported DPPH scavenging activity.…”

First Natural Yeast Strain Trichosporon asahii HZ10 with Robust Flavonoid Productivity and Its Potential Biosynthetic Pathway

“…Therefore, HZ10 was subjected to subsequent further analysis given its good growth performance and the highest productivity of flavonoids. This is the first reported natural flavonoid-producing yeast strain worldwide for only endophytic fungi isolated from medicinal plants − ,, , and a few Streptomyces species , have so far been found to naturally produce flavonoids.…”

“…The DPPH and ABTS radical scavenging activities of samples were evaluated using the DPPH free radical scavenging capacity assay kit and the ABTS free radical scavenging capacity assay kit (Solarbio, China), respectively. The scavenging activity of the hydroxyl radical and the superoxide radical was determined according to the method of Tang et al 7 and Zhao et al, 21 respectively.…”

Optimization of Extraction of Compound Flavonoids from Chinese Herbal Medicines Based on Quantification Theory and Evaluation of Their Antioxidant Activity

“…e difference in the hydroxyl radical scavenging rates of TBFs and PCFs was not significant (P > 0.05). CFs showed excellent hydroxyl radical scavenging capability at all concentrations in a dose-dependent manner, which surpassed the scavenging capabilities of tremella flavonoids (lower than 30%) [34], peony seed meal flavonoids (70.33%) [35], kale flavonoids (under 60%) [36], and bear bile grass flavonoids (73.79%) [37]. e reducing power reflects the potential activity of natural products and phytochemicals.…”