Optimal extraction and fingerprinting of carotenoids by accelerated solvent extraction and liquid chromatography with tandem mass spectrometry

Saha, Supradip; Walia, Suresh; Kundu, Aditi; Sharma, Khushbu; Paul, Ranjit Kumar

doi:10.1016/j.foodchem.2015.01.039

Cited by 52 publications

(27 citation statements)

References 28 publications

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“…The use of higher temperatures during extraction probably enhanced its efficiency and prolongation of the extraction time was unnecessary. Saha et al (), who applied ASE to the analysis of carotenoids from carrots, observed that the influence of static time was considerable at lower temperatures, which is in agreement with results presented in this article.…”

Section: Discussionsupporting

confidence: 92%

Analysis of chloropigments in marine sediments using accelerated solvent extraction (ASE)

Szymczak‐Żyła

2016

Limnology & Ocean Methods

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The application of accelerated solvent extraction (ASE) for the analysis of chloropigments in marine sediment samples was studied. Chloropigments (chlorophyll a, b, c and their derivatives) are a group of natural compounds providing valuable data on the state of the aquatic environment. As these compounds are rather unstable and given the considerable risk that alteration products will form, their analysis is difficult. The new extraction method was optimized by testing both solvents and ASE operating conditions (static time, rinse volume, number of static cycles). The results obtained using an ASE technique were compared with those using an ultrasound‐assisted extraction method. The results presented in this article indicate that the application of ASE gives high chloropigment recoveries, comparable with conventional methods, but in less time, without the use of elevated temperature during extraction. The type of solvent used has the greatest influence on the extraction efficiency while the effects of the other parameters tested are important in the case of organic‐rich sediments. In addition, the influence of freeze‐drying of sediment samples on chloropigments composition and target compound stability in freeze‐dried sediments during storage under different conditions was examined. The results indicate that freeze‐drying does not affect the composition of pigments in the sediment samples and improves reproducibility of results, although extraction of freeze‐dried sediments does require the use of a more polar solvent (95% acetone). Freeze‐dried sediment samples could be stored in a freezer (−20°C) for up to 6 months, without the risk of degradation of the target compounds.

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Section: Discussionsupporting

confidence: 92%

Analysis of chloropigments in marine sediments using accelerated solvent extraction (ASE)

Szymczak‐Żyła

2016

Limnology & Ocean Methods

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“…The ASE method is superior to other traditional extraction methods, and high temperature and high pressure are important parameters for optimizing the ASE process. High temperature can reduce the viscosity of the solvent, increasing its ability to dissolve analytes, and high pressure can maintain solvents in the liquid state, allowing them to pass quickly through the ASE system. However, pressure is typically maintained at 1500 psi and is not considered a key parameter as it has little effect on analyte recovery.…”

Section: Resultsmentioning

confidence: 99%

Optimization of ASE and SPE conditions for the HPLC‐FLD detection of piperazine in chicken tissues and pork

et al. 2019

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Accelerated solvent extraction (ASE) and solid-phase extraction (SPE) conditions were optimized by a high-performance liquid chromatographyfluorescence detector (HPLC-FLD) method for the detection of piperazine in chicken tissues and pork. Piperazine residues were determined by precolumn derivatization with trimethylamine and dansyl chloride. Samples were extracted with 2% formic acid in acetonitrile using an ASE apparatus and purified using a Strata-X-C SPE column. The monosubstituted product of the reaction of piperazine with dansyl chloride was 1-dansyl piperazine (1-DNSpiperazine). Chromatographic separations were performed on an Athena C 18 column (250 × 4.6 mm, id: 5 μm) with gradient elution using ultrapure water and acetonitrile (5:95, V/V) as the mobile phase. The calibration curves showed good linearity over a concentration range of LOQ-200.0 μg/kg with a coefficient of determination (R 2 ) ≥ .9992. The recoveries and relative standard deviations (RSD values) ranged from 78.49% to 97.56% and 1.19% to 5.32%, respectively, across the limit of quantification (LOQ) and 0.5, 1, and 2.0 times the maximum residue limit (MRL; μg/kg). The limits of detection (LODs) and LOQs were 0.96 to 1.85 μg/kg and 3.20 to 5.50 μg/kg, respectively. The method was successfully applied for the validation of animal products in the laboratory.

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“…The extractive process used for the ELPA was ASE, developed to maximize the yield of the extract and simultaneously the content of flavonoids (18). According Pearson et al (2010) (32) and Saha et al (2015) (33), the process is promising, because it offers advantages over other extraction processes such as: easy automation, faster sample analysis, better reproducibility, less solvent required, and sample maintenance in an environment without light and free of oxygen. Accelerated extraction systems also allow the operator to control the temperature, pressure, extraction time and number of extractions, which may increase the number of compounds extracted from the plant when optimized.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the cytotoxic potential of extracts from the genus Passiflora cultived in Brazil against cancer cells

Svf

Âr

et al. 2018

Preprint

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Abstract20 This work aimed to evaluate the cytotoxic potential against cancer cells of Passiflora genus 21 plant species cultivated in Brazil and identify the mechanism of cytotoxicity induced by the 22 most promising extract. Leaf extracts from 14 Passiflora (P.) species were obtained ASE and 23 in vitro cytotoxicity evaluated against cancer cell lines using MTT assay at a single . CC-BY 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

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Optimal extraction and fingerprinting of carotenoids by accelerated solvent extraction and liquid chromatography with tandem mass spectrometry

Cited by 52 publications

References 28 publications

Analysis of chloropigments in marine sediments using accelerated solvent extraction (ASE)

Analysis of chloropigments in marine sediments using accelerated solvent extraction (ASE)

Optimization of ASE and SPE conditions for the HPLC‐FLD detection of piperazine in chicken tissues and pork

Evaluation of the cytotoxic potential of extracts from the genus Passiflora cultived in Brazil against cancer cells

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