Optimal extraction and fingerprint analysis of Cnidii fructus by accelerated solvent extraction and high performance liquid chromatographic analysis with photodiode array and mass spectrometry detections

Gao, Fangyuan; Hu, Yingyu; Ye, Xiaolan; Li, Ji; Zhao, Chen; Fan, Guorong

doi:10.1016/j.foodchem.2013.05.013

Cited by 35 publications

(19 citation statements)

References 17 publications

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“…The monosubstitution of piperazine with dansyl chloride produced 1‐DNS‐piperazine under the selected derivatization conditions in this experiment (temperature, 50°C; time, 20 minutes; 0.12% triethylamine solution), as determined by MS analysis. These results are consistent with previous reports . The mass spectrum of 1‐DNS‐piperazine is shown in Figure .…”

Section: Resultssupporting

confidence: 93%

“…The ASE method is superior to other traditional extraction methods, and high temperature and high pressure are important parameters for optimizing the ASE process. High temperature can reduce the viscosity of the solvent, increasing its ability to dissolve analytes, and high pressure can maintain solvents in the liquid state, allowing them to pass quickly through the ASE system. However, pressure is typically maintained at 1500 psi and is not considered a key parameter as it has little effect on analyte recovery.…”

Section: Resultsmentioning

confidence: 99%

“…However, pressure is typically maintained at 1500 psi and is not considered a key parameter as it has little effect on analyte recovery. Unlike pressure, temperature is considered a key parameter, and it has a large effect on extraction efficiency . The extraction efficiency at 70°C is much higher than that at 60°C or 90°C .…”

Section: Resultsmentioning

confidence: 99%

“…The derivative formed by the reaction of piperazine with benzaldehyde only has UV activity . In this experiment, dansyl chloride was chosen as the derivatization reagent because piperazine contains a secondary amine, and the product reacted with it has a fluorescent group and is structurally stable . The reaction of piperazine with dansyl chloride can produce both monosubstituted and disubstituted products.…”

Section: Resultsmentioning

confidence: 99%

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Optimization of ASE and SPE conditions for the HPLC‐FLD detection of piperazine in chicken tissues and pork

et al. 2019

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Accelerated solvent extraction (ASE) and solid-phase extraction (SPE) conditions were optimized by a high-performance liquid chromatographyfluorescence detector (HPLC-FLD) method for the detection of piperazine in chicken tissues and pork. Piperazine residues were determined by precolumn derivatization with trimethylamine and dansyl chloride. Samples were extracted with 2% formic acid in acetonitrile using an ASE apparatus and purified using a Strata-X-C SPE column. The monosubstituted product of the reaction of piperazine with dansyl chloride was 1-dansyl piperazine (1-DNSpiperazine). Chromatographic separations were performed on an Athena C 18 column (250 × 4.6 mm, id: 5 μm) with gradient elution using ultrapure water and acetonitrile (5:95, V/V) as the mobile phase. The calibration curves showed good linearity over a concentration range of LOQ-200.0 μg/kg with a coefficient of determination (R 2 ) ≥ .9992. The recoveries and relative standard deviations (RSD values) ranged from 78.49% to 97.56% and 1.19% to 5.32%, respectively, across the limit of quantification (LOQ) and 0.5, 1, and 2.0 times the maximum residue limit (MRL; μg/kg). The limits of detection (LODs) and LOQs were 0.96 to 1.85 μg/kg and 3.20 to 5.50 μg/kg, respectively. The method was successfully applied for the validation of animal products in the laboratory.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Optimization of ASE and SPE conditions for the HPLC‐FLD detection of piperazine in chicken tissues and pork

et al. 2019

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“…The results in this study demonstrated that osthole is an important antifungal ingredient of C. fructus. In addition to osthole, there are other major constituents in C. fructus, such as imperatorin, bergapten, and isopimpinellin (21,22). However, these constituents did not show any anti-C. albicans effects either alone or in combination with azoles (data not shown).…”

Section: Discussionmentioning

confidence: 95%

Potent In Vitro Synergism of Fluconazole and Osthole against Fluconazole-Resistant Candida albicans

Chai

Huang

et al. 2017

Antimicrob Agents Chemother

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Osthole is a natural coumarin that exhibits wide biological and pharmacological activities such as neuroprotective, osteogenic, immunomodulation, antitumor, and anti-inflammatory effects. In this study, we investigated the antifungal effects of osthole in vitro. A checkerboard microdilution assay showed that osthole has significant synergistic effect with fluconazole against fluconazole-resistant Candida albicans. Similar results were obtained from a growth curve assay. Meanwhile, XTT reduction assay demonstrated the synergism of fluconazole and osthole against C. albicans biofilm formation. Microarray results showed that the expression of genes involved in the oxidation-reduction process, energy metabolism, and transportation changed significantly after the combined treatment with fluconazole and osthole, and further results showed that endogenous reactive oxygen species (ROS) was significantly increased in the combination group. In conclusion, these results demonstrate the synergism of fluconazole and osthole against fluconazole-resistant C. albicans and indicate that endogenous ROS augmentation might contribute to the synergism of fluconazole and osthole.

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Coumarins: Analytical and Preparative Techniques

Luca

Głowniak

Skalicka‐Woźniak

2018

Encyclopedia of Analytical Chemistry

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Coumarins are nowadays an important group of organic compounds from natural and synthetic sources, possessing a wide range of pharmacological activities. Coumarins comprise a large group of phenolic substances, which occur in plants as secondary metabolites. On account of the significance of this group of compounds, the development of effective methods for their extraction, separation, qualitative, and quantitative analysis, as well as for purification remains of high importance. The purpose of the article is to present information about methods of analysis and separation of this important group of naturally occurring compounds.

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Optimal extraction and fingerprint analysis of Cnidii fructus by accelerated solvent extraction and high performance liquid chromatographic analysis with photodiode array and mass spectrometry detections

Cited by 35 publications

References 17 publications

Optimization of ASE and SPE conditions for the HPLC‐FLD detection of piperazine in chicken tissues and pork

Optimization of ASE and SPE conditions for the HPLC‐FLD detection of piperazine in chicken tissues and pork

Potent In Vitro Synergism of Fluconazole and Osthole against Fluconazole-Resistant Candida albicans

Coumarins: Analytical and Preparative Techniques

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