Optimal culture condition for the production of phenyalanine ammonia lyase from E. coli

Cui, Jiandong; Li, Yan

doi:10.1007/s11814-009-0075-0

Cited by 10 publications

(7 citation statements)

References 18 publications

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“…The specific activity of PAL in E. coli JM109 (pBV-PAL) was higher than that reported by Faulkner et al (1994), it reached 35 U/g dried cell weight (DCW). PAL-specific activity in the recombinant E. coli (contain expression plasmid pBV-PAL) reached 300 U/g (DCW) by using a controlled fed-batch fermentation system in a 5 L jar fermentor (Cui & Li, 2009). However, the yield of recombinant PAL obtained still need to drastically increase for industrial and medical applications.…”

Section: Strategy Of Improving Pal Activitymentioning

confidence: 99%

Biotechnological production and applications of microbial phenylalanine ammonia lyase: a recent review

Cui

Qiu

Fan

et al. 2013

Critical Reviews in Biotechnology

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Phenylalanine ammonia lyase (PAL) catalyzes the nonoxidative deamination of l-phenylalanine to form trans-cinnamic acid and a free ammonium ion. It plays a major role in the catabolism of l-phenylalanine. The presence of PAL has been reported in diverse plants, some fungi, Streptomyces and few Cyanobacteria. In the past two decades, PAL has gained considerable significance in several clinical, industrial and biotechnological applications. Since its discovery, much knowledge has been gathered with reference to the enzyme's importance in phenyl propanoid pathway of plants. In contrast, there is little knowledge about microbial PAL. Furthermore, the commercial source of the enzyme has been mainly obtained from the fungi. This study focuses on the recent advances on the physiological role of microbial PAL and the improvements of PAL biotechnological production both from our laboratory and many others as well as the latest advances on the new applications of microbial PAL.

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Section: Strategy Of Improving Pal Activitymentioning

confidence: 99%

Biotechnological production and applications of microbial phenylalanine ammonia lyase: a recent review

Cui

Qiu

Fan

et al. 2013

Critical Reviews in Biotechnology

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“…Fed-batch culture under constant substrate feeding rate is a simple way to improve microbial biomass and product formation [18]. First, a high-cell-density culture of C. utilis SZU 07-01 was conducted by glucose feeding after 12 h under different constant feeding rate of 4.0, 5.0, 6.7 and 8.0 g/(L·h) until the total glucose concentration of 150 g/L to find if there existed a suitable glucose feeding rate (Fig.…”

Section: Fed-batch Glutathione Production Under Constant Glucose Feedmentioning

confidence: 99%

A novel strategy on the high-cell-density cultivation of Candida utilis for the enhanced production of glutathione

Nie

Wei

Shao

et al. 2010

Korean J. Chem. Eng.

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Efficient glutathione production by high-cell-density cultivation of Candida utilis was investigated. A series of batch glutathione fermentations were carried out and the optimal initial glucose concentration was found to be about 26 g/L. Then, fed-batch fermentation under diverse feeding strategies was used to enhance glutathione production with a total glucose concentration of 150 g/L. Constant glucose feeding strategy cannot meet the requirement of cells at the late period of feeding, while exponential glucose feeding strategy cannot satisfy the needs of cells at the beginning of feeding. Based on the results above, a polynomial glucose feeding strategy was developed to provide enough glucose for cells along with the cultivation, under which both the cell and glutathione productivity were satisfactorily improved. Furthermore, fed-batch fermentation under this strategy with a total glucose concentration of 200 g/L was successfully performed, the dry cell weight and glutathione concentration reached 91.2 g/L and 825 mg/L, respectively.

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“…The harvested cells were suspended in the double distilled water (2%, w/w) as cells suspension [18].…”

Section: Preparation Of the Cell Suspensionmentioning

confidence: 99%

Production of hydrocortisone by Absidia coerulea in moderate pressure bioconversion system

Shi

Cui

2009

Korean J. Chem. Eng.

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The effects of moderate pressure (0.1-2.5 MPa) on viability, cell membrane permeability and catalyzing activity of Absidia coerulea for RSA were investigated. A new method for improving the production of Hydrocortisone (HC) from 17α-hydroxypregn-4-en-3, 20-dione-21-acetate by Absidia coerulea in moderate pressure was developed. The results showed that the morphology of Abasidia coerulea mycelium was changed in moderate pressure, Absidia coerulea mycelium seemed to be loosed, and cell membrane permeability of Abasidia coerulea mycelium was improved. However, the viability of Abasidia coerulea mycelium could keep high level. Moreover, the yield of HC was improved over 1.25-fold as compared with that of the control (untreated cells), to give the yield of HC as 350 mg/l, when the Abasidia coerulea mycelium was treated with 0.5 Mpa the atmosphere as the pressure media. Especially, the production of HC with atmosphere as the pressure media (0.5 MPa) could be increased by the addition of H 2 O 2 (60 mmol/l); the relative yield of HC in moderate pressure was enriched by over 4.5% in comparison with the control. The major composition of bioconverted mixture was reduced. It was indicated that the new approach (moderate pressure) obtained in this work possessed a high potential for the industrial production of HC.

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Optimal culture condition for the production of phenyalanine ammonia lyase from E. coli

Cited by 10 publications

References 18 publications

Biotechnological production and applications of microbial phenylalanine ammonia lyase: a recent review

Biotechnological production and applications of microbial phenylalanine ammonia lyase: a recent review

A novel strategy on the high-cell-density cultivation of Candida utilis for the enhanced production of glutathione

Production of hydrocortisone by Absidia coerulea in moderate pressure bioconversion system

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