Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in escherichia coli K12

Chlorate toxicity in Aspergillus nidulans: The selection and characterisation of chlorate resistant mutants

“…Conidiospores were treated with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) according to the method of Adelberg, Mandel and Chen (1965) as modified by Alderson and Hartley (1969), so that survival was between 5 and 15 per cent.…”

Mutants of Escherichia coli K12 Permeable to Haemin

“…The procedure gave sterile solutions which were stored at -18 "C and added aseptically to give concentrations of 25 mg 1-1 in CM and 10 mg 1-1 in GM and GMA. N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) was used by the method of Adelberg et al (1965). The following K12 strains were used: AB1515 F-leu-13 lacY5proCl4purE27 trp-21 mil-7 rpsL xyl-11 mtl-5 thi-9 (strain 13-6 of Schwartz, 1964); AB1621 F-ara-14 tfrA lac-1 tsx-57gal-2 rpsL xyl-5 mtl-1 thi-1 A+ (Adelberg et al, 1965); AB312 Hfr thr leu rpsL (Taylor & Adelberg, 1960).…”

“…N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) was used by the method of Adelberg et al (1965). The following K12 strains were used: AB1515 F-leu-13 lacY5proCl4purE27 trp-21 mil-7 rpsL xyl-11 mtl-5 thi-9 (strain 13-6 of Schwartz, 1964); AB1621 F-ara-14 tfrA lac-1 tsx-57gal-2 rpsL xyl-5 mtl-1 thi-1 A+ (Adelberg et al, 1965); AB312 Hfr thr leu rpsL (Taylor & Adelberg, 1960). Strain MLMlO6 was a 5-ALA-requiring mutant of AB1621 carrying the new allele hernAlO6.…”

Alternate requirement for pyridoxine or isoleucine in mutants of Escherichia coli

“…Cultures were incubated at 37" C on a rotary shaker. Auxotrophs were induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) [3] . Cross-feeding tests were performed by the method of Dempsey and Pachler [4] .…”