Optimal conditions for mutagenesis by ozone in Escherichia coli K12

Hamelin, Claude; Chung, Young Sup

doi:10.1016/0027-5107(74)90175-4

Cited by 51 publications

(16 citation statements)

References 34 publications

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“…Levels of 0.001 ppm were not high enough to show the same results. There is some variation in the literature regarding what level of ozone is required for bactericidal purposes, but it is generally in the range of 1 ppm and above [34,35]. In the current experiment, a significant difference in bacterial numbers was not noted.…”

Section: Resultscontrasting

confidence: 70%

Effect of adding ozone into an intensive broiler production unit on performance, mortality, ammonia levels, and bacterial levels as compared with a non-ozone-treated broiler unit

Schwean-Lardner

Dahiya

Olkowski

et al. 2009

Journal of Applied Poultry Research

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Ozone is a very unstable and reactive molecule that can rapidly oxidize many organic substances and is often used for odor control. It is also known to be an effective biocide at high dose levels. Despite the lack of scientific evidence linking ozone to reduced aerial ammonia at levels that are safe for birds and humans, there is still an interest in the use of the product for commercial production poultry units. The objective of this experiment was to determine the effect of adding atmospheric ozone at a target level of 0.05 ppm (level achieved, 0.03 ± 0.017 ppm) to rooms housing broiler chickens. Female broiler chickens were grown in either an untreated environment or an environment with added ozone (3 rooms of 5 replicate pens, each containing 110 birds/treatment). Bird weight and feed consumption were recorded on a pen basis at 21 and 40 d of age; mortalities were collected daily, and necropsies were performed. Air samples were collected and plated on appropriate media to count total aerobic bacteria at 11, 19, and 34 d of age and enterobacteria at 34 d. Atmospheric ammonia levels were measured on d 15, 20, 28, 32, and 38. A significant (P ≤ 0.05) improvement in feed conversion (feed:gain ratio) when corrected for mortality was noted in the ozone-treated birds (1.808 vs. 1.870). Birds exposed to ozone grew significantly slower (1.938 vs. 2.053 kg of BW gain), consumed less feed (3.695 vs. 3.953 kg), and had a higher mortality (11.46 vs. 7.33%) and condemnation percentages (10.36 vs. 3.39%) than nontreated broilers. The addition of ozone caused no significant decrease in ammonia level or total bacterial count. The major increases in morbidity and mortality of the birds subjected to ozone make ozone gas unacceptable for use in a commercial broiler unit and raise serious health issues for both producers and birds.

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Section: Resultscontrasting

confidence: 70%

Effect of adding ozone into an intensive broiler production unit on performance, mortality, ammonia levels, and bacterial levels as compared with a non-ozone-treated broiler unit

Schwean-Lardner

Dahiya

Olkowski

et al. 2009

Journal of Applied Poultry Research

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“…Hammelin and Chung described the appearance of four mutant strains of E coli growing in 0*1 ppm ozone. 4 Our work was carried out to examine the possible use of this small ozone generator to decontaminate rooms containing immunosuppressed patients. Ozone, at high concentration, has been used to disinfect rooms prior to patient occupation,2 but these concentrations are toxic to man.78 Mortality rates are increased in exercising mice exposed to 0-3 ppm ozone for three hours and then challenging them with an aerosol of Strep pyogenes.9 In mice exposed…”

Section: Resultsmentioning

confidence: 99%

Ozone killing action against bacterial and fungal species; microbiological testing of a domestic ozone generator.

Dyas¹,

Boughton²,

Das³

1983

Journal of Clinical Pathology

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BirminghamSUMMARY The action of ozone generated from a small domestic device was examined with a view to using it in clinical isolation units accommodating immunosuppressed patients. Over a six-hour period in an average size room the device did not generate sufficient ozone to suppress bacterial and fungal growth. A useful bactericidal action, against a variety of human pathogens was achieved with ozone concentrations between 0-3 to 0-9 ppm. Bactericidal ozone concentrations are close to the limit permitted for human exposure however and further experiments are indicated.Ozone has well documented bactericidal properties which are of use in the decontamination of uninhabited "bioclean" areas.' 2 The purpose of the present study was to determine the effectiveness of an inexpensive domestic ozone generator in maintaining an ultra clean environment for immunosuppressed patients. Material and methodsThe Miles and Misra technique of bacterial enumeration3 was modified as follows. Peptone water suspensions were made from plate cultures of E coli, Proteus sp, Pseudomonas aeruginosa, Serratia sp, three strains of Staphylococcus aureus, Candida albicans and Aspergillus fumigatus. Subsequent tenfold dilutions were made in sterile water. Two separate 20 Al drops of each dilution were then delivered on to each of two predried plates. Blood agar was used, except for Proteus sp where cysteine lactose electrolyte deficient (CLED) agar was used and for Aspergillus fumigatus where Sabouraud's agar was used. One plate was exposed to ozone for 4 h, the duplicate being placed in an ozone free environment. After 4 h all plates were incubated overnight at 37°C and the colonies counted using those dilutions which gave discrete colonies on both plates. All experiments were performed in duplicate.Ozone was produced using a small "Airbracer"

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“…In this respect it should be noted that the frameshift mutants utilized in our studies are particularly sensitive to mutagenesis by a number of polycyclic carcinogens (16), at least some of which are also associated with the production of lipid peroxidation during their metabolism (5). An additional area of possible pertinence, and the original impetus for this study, concerns the observation of chromosomal aberrations in the circulating lymphocytes of man and Chinese hamsters following inhalation of relatively low levels of ozone (17), an air pollutant known to be mutagenic in animals and bacteria (18). It is unlikely that this extremely reactive oxidant gas, which produces lung lipid peroxidation (19), can itself reach directly into the nucleus of circulating lymphocytes.…”

mentioning

confidence: 99%

Mutagenicity of Malonaldehyde, a Decomposition Product of Peroxidized Polyunsaturated Fatty Acids

Mukai

Goldstein

1976

Science

349

121

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Incubation of histidine requiring auxotrophs of the bacterium Salmonella typhimurium with malonaldehyde, a three-carbon dialdehyde, produced an increased number of revertants in specific strains. Mutagenesis was only observed in frameshift mutants with normal excision repair and did not occur in those base-pair substitution mutants tested. The results are consistent with the cross-linking of bacterial DNA by malonaldehyde leading to mutagenesis expressed through the error-prone repair system.

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Optimal conditions for mutagenesis by ozone in Escherichia coli K12

Cited by 51 publications

References 34 publications

Effect of adding ozone into an intensive broiler production unit on performance, mortality, ammonia levels, and bacterial levels as compared with a non-ozone-treated broiler unit

Effect of adding ozone into an intensive broiler production unit on performance, mortality, ammonia levels, and bacterial levels as compared with a non-ozone-treated broiler unit

Ozone killing action against bacterial and fungal species; microbiological testing of a domestic ozone generator.

Mutagenicity of Malonaldehyde, a Decomposition Product of Peroxidized Polyunsaturated Fatty Acids

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