Optimal conditions for induction of certain mutation types inBrevibacterium flavum by N-Methyl-N’-nitro-N-nitrosoguanidine

Koníčék, J.; Koníčková-Radochová, M.; Strnadová, M.; Chaloupka, J.; Wolf, Anja; Rytíř, V.; Hubáček, J.

doi:10.1007/bf02925841

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Mutagenesis and Enrlchment By Amplciilin1

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1994

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“…With Gram-negative bacteria ampicillin enrichment is an efficient technique (Davis 1948;Lederberg and Zinder 1948). In the Gram-positive bacterium, Brevibacteriurn Javum, vancomycin at a concentration of 100 mg I-' was used and the survival rate was only 0.001% after 12 h (Konicek et al 1988).…”

Section: Mutagenesis and Enrlchment By Amplciilinmentioning

confidence: 99%

Selection and biochemical studies of pyrimidine‐requiring mutants of Lactobacillus plantarum

Masson¹,

Kammerer²,

Hubert³

1994

Journal of Applied Bacteriology

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Mutagenesis and mutant enrichment in Lactobacillus plantarum, a lactic acid bacterium used in silage, sauerkraut and sausage fabrication, were studied. In optimal conditions, auxotrophic mutants were obtained that permitted investigation of the de novo pyrimidine biosynthesis. Uracil‐requiring mutants were characterized for their enzymatic defect, in aspartate transcarbamylase (ATCase), dihydro‐orotase (DHOase), dihydro‐orotate dehydrogenase (DHOdehase), orotidine monophosphate pyrophosphorylase (OMPppase) or in orotidine monophosphate decarboxylase (OMPdecase). The five enzymatic activities are totally repressed by uracil in the growth medium.

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Section: Mutagenesis and Enrlchment By Amplciilinmentioning

confidence: 99%