Optimal conditions for heart cell cryopreservation for transplantation

Yokomuro, Hiroki; Mickle, Donald A.G.; Weisel, Richard D.; Li, Ren‐Ke

doi:10.1007/978-1-4757-4712-6_14

Cited by 6 publications

(4 citation statements)

References 11 publications

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“…For example, cryopreservation of 4‐mm slices of ovarian tissue resulted in 34% normal follicle recovery compared with 56% for smaller tissue slices (2‐mm slices; Ferreira et al , ). Similarly, for fetal myocardium, cell yields were greater for tissue frozen in 0.2‐mm 3 pieces compared with 2‐mm 3 or 6‐mm 3 pieces (Yokomuro et al , ). For these reasons, we chose to attempt cryopreservation of only very small tissue pieces but still saw slight alterations to tissue architecture, which likely rules out simply re‐transplanting cryopreserved lacrimal gland tissue.…”

Section: Discussionmentioning

confidence: 97%

Cryopreservation and hypothermic storage of lacrimal gland: towards enabling delivery of regenerative medicine therapies for treatment of dry eye syndrome

Massie

Spaniol

Geerling

et al. 2016

J Tissue Eng Regen Med

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Severe dry eye syndrome (DES) can cause painful loss of vision and may result from lacrimal gland dysfunction. Current treatments are palliative, so a causative therapy is desirable. The ability to (cryo)preserve lacrimal gland tissue or epithelial cells would simplify this. Here, lacrimal gland tissue was cryopreserved in 10% dimethylsulphoxide in liquid nitrogen, or stored at 4 °C in culture medium for up to 7 days, and compared with fresh tissue using immunohistochemistry. Cultures were initiated from fresh and stored tissue, and cells characterised in P1 for proliferation (WST-1), colony-forming efficiency (CFE) and secretory capacity (immunocytochemistry and β-hexosaminidase activity assay). Tissue stored for > 3 days at 4 °C displayed grossly altered tissue architecture when compared with fresh tissue, decreased acinus density and increased caspase-3 activity. Cryopreserved tissue showed less obvious signs of damage without caspase-3 activation. Storage at 4 °C and cryopreservation delayed epithelial outgrowth compared with that from fresh tissue initially (p < 0.05) but, by day 9, all explants showed comparable outgrowth (~90%), except tissue stored at 4 °C for 3 or 7 days (p < 0.05 compared with fresh tissue). Epithelial cell yields per explant were similar from fresh and stored tissue, apart from tissue stored at 4 °C for 7 days (p < 0.01). In P1, epithelial cells from fresh and stored tissue were largely equivalent in terms of: proliferation; CFE (~21%); Rab3D, HexA and lysozyme expression; mucin production; and β-hexosaminidase activity. These data demonstrate that cryo(preservation) of lacrimal gland tissue and cells is possible, which may enable use of autologous cells in regenerative medicine approaches to treating DES. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 97%

Cryopreservation and hypothermic storage of lacrimal gland: towards enabling delivery of regenerative medicine therapies for treatment of dry eye syndrome

Massie

Spaniol

Geerling

et al. 2016

J Tissue Eng Regen Med

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“…Studies on the cryopreservation of spermatozoa have now linked molecular based stress responses and the loss of acrosomal and motility functions. Other studies have now associated negative effects of cryopreservation on the impairment of biochemical functionality in hepatocytes [ 22 , 64 ] and cardiomyocytes [ 57 ]. These studies have helped to further our understanding and increase our recognition of the downstream effects cryopreservation may have on cellular function.…”

Section: Post-storage Outcomementioning

confidence: 96%

“…The literature contains numerous reports citing high post-thaw cell viability and function [ 4 ]. Further examination of these studies, however, reveals that in many cases there are signifi cant compromises in function post-thaw in cell systems such as hepatocytes [ 22 , 54 , 55 ], pancreatic islets [ 56 ], cardiac cells [ 57 ], blood cells [ 58 ], and stem cells [ 59 ]. Abrahamsen et al [ 60 ] used fl ow cytometry to assess sample quality levels (apoptosis and necrosis) following cryopreservation as a means of establishing dosing parameters for cancer patients the cryopreservation process significantly affected the level of CD34 + expressing cells in PBMC samples.…”

Section: Post-storage Outcomementioning

confidence: 99%

Biobanking: The Future of Cell Preservation Strategies

Baust

Corwin

VanBuskirk

2015

Advances in Experimental Medicine and Biology

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With established techniques cryopreservation is often viewed as an "old school" discipline yet modern cryopreservation is undergoing another scientific and technology development growth phase. In this regard, today's cryopreservation processes and cryopreserved products are found at the forefront of research in the areas of discovery science, stem cell research, diagnostic development and personalized medicine. As the utilization of cryopreserved cells continues to increase, the demands placed on the biobanking industry are increasing and evolving at an accelerated rate. No longer are samples providing for high immediate post-thaw viability adequate. Researchers are now requiring samples where not only is there high cell recovery but that the product recovered is physiologically and biochemically identical to its pre-freeze state at the genominic, proteomic, structural, functional and reproductive levels. Given this, biobanks are now facing the challenge of adapting strategies and protocols to address these needs moving forward. Recent studies have shown that the control and direction of the molecular response of cells to cryopreservation significantly impacts final outcome. This chapter provides an overview of the molecular stress responses of cells to cryopreservation, the impact of the apoptotic and necrotic cell death continuum and how studies focused on the targeted modulation of common and/or cell specific responses to freezing temperatures provide a path to improving sample quality and utility. This line of investigation has provided a new direction and molecular-based foundation guiding new research, technology development and procedures. As the use of and the knowledge base surrounding cryopreservation continues to expand, this path will continue to provide for improvements in overall efficacy and outcome.

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“…While cryopreservation of a single cell suspension is a standard and reliable process, cryopreservation of larger or more complex tissues is more challenging. 8 – 11 During the freezing process, the formation of ice crystals (intra- and extracellular) can cause cellular damage. 12 In tissues, as water begins to crystallize in the extracellular space, an ionic gradient forms across the cell membrane, causing an efflux of water from inside to outside the cells.…”

Section: Introductionmentioning

confidence: 99%

Method for manufacture and cryopreservation of cartilage microtissues

Shajib,

Futrega,

Franco

et al. 2023

J Tissue Eng

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The financial viability of a cell and tissue-engineered therapy may depend on the compatibility of the therapy with mass production and cryopreservation. Herein, we developed a method for the mass production and cryopreservation of 3D cartilage microtissues. Cartilage microtissues were assembled from either 5000 human bone marrow-derived stromal cells (BMSC) or 5000 human articular chondrocytes (ACh) each using a customized microwell platform (the Microwell-mesh). Microtissues rapidly accumulate homogenous cartilage-like extracellular matrix (ECM), making them potentially useful building blocks for cartilage defect repair. Cartilage microtissues were cultured for 5 or 10 days and then cryopreserved in 90% serum plus 10% dimethylsulfoxide (DMSO) or commercial serum-free cryopreservation media. Cell viability was maximized during thawing by incremental dilution of serum to reduce oncotic shock, followed by washing and further culture in serum-free medium. When assessed with live/dead viability dyes, thawed microtissues demonstrated high viability but reduced immediate metabolic activity relative to unfrozen control microtissues. To further assess the functionality of the freeze-thawed microtissues, their capacity to amalgamate into a continuous tissue was assess over a 14 day culture. The amalgamation of microtissues cultured for 5 days was superior to those that had been cultured for 10 days. Critically, the capacity of cryopreserved microtissues to amalgamate into a continuous tissue in a subsequent 14-day culture was not compromised, suggesting that cryopreserved microtissues could amalgamate within a cartilage defect site. The quality ECM was superior when amalgamation was performed in a 2% O2 atmosphere than a 20% O2 atmosphere, suggesting that this process may benefit from the limited oxygen microenvironment within a joint. In summary, cryopreservation of cartilage microtissues is a viable option, and this manipulation can be performed without compromising tissue function.

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Optimal conditions for heart cell cryopreservation for transplantation

Cited by 6 publications

References 11 publications

Cryopreservation and hypothermic storage of lacrimal gland: towards enabling delivery of regenerative medicine therapies for treatment of dry eye syndrome

Cryopreservation and hypothermic storage of lacrimal gland: towards enabling delivery of regenerative medicine therapies for treatment of dry eye syndrome

Biobanking: The Future of Cell Preservation Strategies

Method for manufacture and cryopreservation of cartilage microtissues

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