Optimal activation methods for maximizing the concentrations of platelet-derived growth factor-BB and transforming growth factor-β1 in equine platelet-rich plasma

Fukuda, Kentaro; Kuroda, Taisuke; Tamura, Norihisa; Mita, Hiroshi; Kasashima, Yoshinori

doi:10.1292/jvms.20-0167

Cited by 9 publications

(2 citation statements)

References 35 publications

(50 reference statements)

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“…However, for bacterial flow cytometric analyzes in our study, these two solutions were found to be insufficient to lyse platelets. It was aimed to apply a platelet lysis method that can be easily used in every laboratory (Fukuda et al 2020 ; Rodrigues et al 2022 ; Kandoi et al 2018 ). In our literature review, it was seen that there were a few studies using the freeze-thaw method to achieve platelet lysis.…”

Section: Methodsmentioning

confidence: 99%

Use of flow cytometry method to detect contaminations of platelet suspensions

Bolat,

Hatipoğlu,

Köroğlu

et al. 2024

World J Microbiol Biotechnol

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In this study, it was aimed to investigate bacterial contamination in apheresis platelet suspensions (APS) by automated blood culture system and flow cytometry method (FCM).33 spiked APS each using 11 bacterial strains (5 standard strains, 6 clinical isolates), were prepared in three different dilutions (1–10, 10–50, 50-100 cfu/mL), incubated in two different temperatures (35–37 °C and 22–24 °C) and different incubation times (18–96 h) evaluated by FCM. This three different dilutions were also inoculated into special platelet culture bottles (BacT/ALERT® BPA) and loaded into the blood culture system. Additionally 80 APSs routinely prepared in the Transfusion Center were evaluated by both FCM and the blood culture system. Platelets were lysed by freeze-thaw method.All spiked samples were positive with BacT/ALERT® BPA in 12–18 h. In 96 h incubation at 22–24 °C, the presence of bacteria was detected by FCM in all other samples (31/33) except low dilutions (1–10 and 10–100 CFU/ml) of K.pneumoniae standard strain. In the 35–37 °C, the presence of bacteria was detected by FCM in all samples (33/33) after 48 h of incubation. In routine APS one sample detected as positive (Bacillus simplex) with BacT/ALERT® BPA and no positivity was detected by FCM.The freeze-thaw method, which we have optimized for the lysis of platelets, is very practical and can be easily applied. The BacT/ALERT® system has been found to be very sensitive in detecting bacterial contamination in PSs. Flow cytometry method has been found to be successful, fast, easy to use and low cost in detecting bacterial contamination in PSs.

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Section: Methodsmentioning

confidence: 99%

Use of flow cytometry method to detect contaminations of platelet suspensions

Bolat,

Hatipoğlu,

Köroğlu

et al. 2024

World J Microbiol Biotechnol

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“…71 Double freeze-and-thaw cycles have been suggested to be the optimal external activation method. 72 As for in vivo studies, there is no compelling evidence indicating whether activation is necessary. In the current review, six in vivo studies used platelet lysates and four studies used freeze-and-thaw cycles to activate platelet isolates.…”

Section: Summary Of In Vivo Studiesmentioning

confidence: 99%

A systematic review and meta‐analysis of the efficacy of platelet‐rich plasma products for treatment of equine joint disease

Peng,

Yang,

Labens

et al. 2024

Equine Veterinary Journal

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BackgroundEquine joint disease including septic arthritis (SA) and osteoarthritis (OA) is a critical cause of equine lameness. Platelet‐rich plasma (PRP) is one of the most popular regenerative therapies to treat equine OA, even SA, but the evidence in support of the treatment is conflicting.ObjectivesThe aim of the study was to systematically review the current evidence on PRP products used for SA and OA, as well as the efficacy of PRP products as treatment for OA on the basis of a meta‐analysis of the available literature.Study designSystematic review and meta‐analysis.MethodsA systematic search of relevant databases (PubMed, Web of Science, Scopus) was performed to identify studies published from 2013 to 2023, in accordance with the PRISMA guidelines. Randomised controlled trials, non‐randomised trials and controlled laboratory studies that used at least one type of PRP products were included. Dichotomous outcomes were presented using odds ratios (ORs) and 95% confidence intervals (95% CIs).ResultsA total of 21 publications were identified in the systematic review and 5 of them in the meta‐analysis. These publications involved various types of PRP products and reported different outcomes. Although most of the studies were associated with a high risk of bias, the overall estimated effect was consistent with a significant improvement in the PRP products treatment group compared with the control group (OR: 15.32; 95% CI: 3.00–78.15; p < 0.05). There was a significant improvement in clinical performance outcomes between the groups (OR: 36.64; 95% CI: 3.69–364.30; p < 0.05).ConclusionPRP products as intra‐articular treatment are likely efficacious for treatment of equine OA and have potential for treating SA. These conclusions might be affected by the limited number of randomised controlled studies and high variability of different types of PRP products. To better evaluate the efficacy of PRP, a widely recognised classification system and the utilisation of randomised, blinded, equivalency or non‐inferiority trials are required.

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