“…These particles are trapped at the focal point of a tightly focused by optical gradient forces. Several applications of this non-contact particle manipulation have been considered ranging from movement tracking [8][9][10], application or detection of small forces [11,12], altering cell membranes [13][14][15], cells sorting [16][17][18] and particles manipulation [19][20][21][22][23][24], to name a few. This last application, which is the most popular one, includes dielectric spheres, viruses, bacteria, organelles, or DNA stands manipulation.…”