Optically Clear and Resilient Free-Form µ-Optics 3D-Printed via Ultrafast Laser Lithography

Jonušauskas, Linas; Gailevičius, Darius; Mikoliūnaitė, Lina; Sakalauskas, Danas; Šakirzanovas, Simas; Juodkazis, Saulius; Malinauskas, Mangirdas

doi:10.3390/ma10010012

Cited by 126 publications

(102 citation statements)

References 65 publications

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“…We attribute the bright autofluorescence to the photoinitiator employed in the commercial IP‐DiP resin. It is relevant to point out how this problematic may be fixed in future by using customized materials inspired by recently reported progresses concerning the fabrication of structures based on non‐photosensitized polymers …”

Section: Resultsmentioning

confidence: 99%

Multiphoton Direct Laser Writing and 3D Imaging of Polymeric Freestanding Architectures for Cell Colonization

Accardo

Blatché

Courson

et al. 2017

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The realization of 3D architectures for the study of cell growth, proliferation, and differentiation is a task of fundamental importance for both technological and biological communities involved in the development of biomimetic cell culture environments. Here we report the fabrication of 3D freestanding scaffolds, realized by multiphoton direct laser writing and seeded with neuroblastoma cells, and their multitechnique characterization using advanced 3D fluorescence imaging approaches. The high accuracy of the fabrication process (≈200 nm) allows a much finer control of the micro- and nanoscale features compared to other 3D printing technologies based on fused deposition modeling, inkjet printing, selective laser sintering, or polyjet technology. Scanning electron microscopy (SEM) provides detailed insights about the morphology of both cells and cellular interconnections around the 3D architecture. On the other hand, the nature of the seeding in the inner core of the 3D scaffold, inaccessible by conventional SEM imaging, is unveiled by light sheet fluorescence microscopy and multiphoton confocal imaging highlighting an optimal cell colonization both around and within the 3D scaffold as well as the formation of long neuritic extensions. The results open appealing scenarios for the use of the developed 3D fabrication/3D imaging protocols in several neuroscientific contexts.

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Section: Resultsmentioning

confidence: 99%

Multiphoton Direct Laser Writing and 3D Imaging of Polymeric Freestanding Architectures for Cell Colonization

Accardo

Blatché

Courson

et al. 2017

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“…The scanning speed adopted in the processing of the microtubes is 30 µm s −1 and the laser power range for the microstructures with different parameters is 50 to 150 mW (for details see Figure S2 in Supporting Information for details). Taking the intensity of a Bessel beam ( n = 25, r 0 = 600) for example, the 120 mW of average optical power (measured before the objective) results in the 0.36 TW cm −2 intensity/irradiance; transmission of the optical elements was taken into account . After polymerization, the sample was developed in 1‐propanol for 1 h until all of the unpolymerized parts were washed away.…”

Section: Methodsmentioning

confidence: 99%

Dimension‐Controllable Microtube Arrays by Dynamic Holographic Processing as 3D Yeast Culture Scaffolds for Asymmetrical Growth Regulation

Yang

et al. 2017

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Transparent microtubes can function as unique cell culture scaffolds, because the tubular 3D microenvironment they provide is very similar to the narrow space of capillaries in vivo. However, how to realize the fabrication of microtube-arrays with variable cross-section dynamically remains challenging. Here, a dynamic holographic processing method for producing high aspect ratio (≈20) microtubes with tunable outside diameter (6-16 µm) and inside diameter (1-10 µm) as yeast culture scaffolds is reported. A ring-structure Bessel beam is modulated from a typical Gaussian-distributed femtosecond laser beam by a spatial light modulator. By combining the axial scanning of the focused beam and the dynamic display of holograms, dimension-controllable microtube arrays (straight, conical, and drum-shape) are rapidly produced by two-photon polymerization. The outside and inside diameters, tube heights, and spatial arrangements are readily tuned by loading different computer-generated holograms and changing the processing parameters. The transparent microtube array as a nontrivial tool for capturing and culturing the budding yeasts reveals the significant effect of tube diameter on budding characteristics. In particular, the conical tube with the inside diameter varying from 5 to 10 µm has remarkable asymmetrical regulation on the growth trend of captured yeasts.

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“…The channels were sealed with 150 µm cover glass slide. For the filter mesh fabrication a hybrid organic-inorganic photopolymer SZ2080 was chosen as it exhibits high mechanical strength, 11 wide fabrication window 12 and, if need arises, could be easily combined with organic 13 or inorganic 14 additives for increased functionality. It was mixed with 1 wt.% photoinitiator 2-benzyl-2-dimethylamino-1-(4-morpholinophenyl)-butanone-1 (also known as Irgacure 369).…”

Section: Methodsmentioning

confidence: 99%

Hybrid Subtractive-Additive-Welding Microfabrication for Lab-on-Chip (LOC) Applications via Single Amplified Femtosecond Laser Source

Jonušauskas¹,

Rekštytė²,

Buividas³

et al. 2017

Preprint

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Abstract. An approach employing ultrafast laser hybrid subtractive-additive microfabrication combining ablation, 3D nanolithography and welding is proposed for the realization of Lab-On-Chip (LOC) device. Single amplified Yb:KGW fs-pulsed laser source is shown to be suitable for fabricating microgrooves in glass slabs, polymerization of fine-meshes filter out of hybrid organic-inorganic photopolymer SZ2080 inside them, and, lastly, sealing the whole chip with cover glass into a single monolithic piece. The created microfluidic device proved its particle sorting function by separating 1 µm and 10 µm polystyrene spheres in a mixture. All together, this shows that fs-laser microfabrication technology is a flexible and versatile tool for the manufacturing of mesoscale multi-material LOC devices.

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Optically Clear and Resilient Free-Form µ-Optics 3D-Printed via Ultrafast Laser Lithography

Cited by 126 publications

References 65 publications

Multiphoton Direct Laser Writing and 3D Imaging of Polymeric Freestanding Architectures for Cell Colonization

Multiphoton Direct Laser Writing and 3D Imaging of Polymeric Freestanding Architectures for Cell Colonization

Dimension‐Controllable Microtube Arrays by Dynamic Holographic Processing as 3D Yeast Culture Scaffolds for Asymmetrical Growth Regulation

Hybrid Subtractive-Additive-Welding Microfabrication for Lab-on-Chip (LOC) Applications via Single Amplified Femtosecond Laser Source

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