Optically amended biosynthesized crystalline copper-doped ZnO for enhanced antibacterial activity

Mengistu, Adam; Naimuddin, Mohammed; Abebe, Buzuayehu

doi:10.1039/d3ra04488b

Cited by 6 publications

(3 citation statements)

References 69 publications

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“…The assay for EO was similarly performed in microtiter plates except that the volumes were reduced. The %RSA was calculated according to the following formula % RSA = A 0 − A 1 A 0 × 100 % where %RSA is the percent radical scavenging activity, A 0 is the absorbance of the blank DPPH, and A 1 is the absorbance of sample. , …”

Section: Methodsmentioning

confidence: 99%

“…Several approaches have been developed including antibodies, vaccines, antimicrobial peptides (AMPs), probiotics, plant natural products, and nanobiotechnology. 2,20 Natural products derived from plants and microbes have received particular attention. 21 The natural products in different parts of plants are comprised of phenols, tannins, flavonoids, saponins, terpenoids, essential oils, alkaloids, steroids, lectins, polypeptides, etc.…”

Section: Introductionmentioning

confidence: 99%

“…The discovery and development of antibiotics during the “golden period” (1930–1960s) have saved millions of lives; however, the difficulty to maintain the pace of antibiotic discovery with the emerging and re-emerging resistant pathogens has mounted the current massive challenge to contain and treat infections. , Therefore, there is an urgent need to search for and discover novel alternate treatment strategies to combat AMR and MDR. Several approaches have been developed including antibodies, vaccines, antimicrobial peptides (AMPs), probiotics, plant natural products, and nanobiotechnology. , Natural products derived from plants and microbes have received particular attention . The natural products in different parts of plants are comprised of phenols, tannins, flavonoids, saponins, terpenoids, essential oils, alkaloids, steroids, lectins, polypeptides, etc.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical Composition, Antioxidant, Antimicrobial, Antibiofilm, and Antiquorum Sensing Potential of Methanol Extract and Essential Oil from Acanthus polystachyus Delile (Acanthaceae)

Getahun,

Nesru,

Ahmed

et al. 2023

ACS Omega

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The evolution of microbes in response to conventional antimicrobials leads to antimicrobial resistance (AMR) and multidrug resistance (MDR), and it is a global threat to public health. Natural products are possible solutions to this massive challenge. In this study, the potential of Acanthus polystachyus extracts was investigated for phytochemical composition and biological properties as antimicrobials. Gas chromatography-mass spectra (GC-MS) analysis of methanol extract (ME) and essential oil (EO) detected 79 and 20 compounds, respectively. The major compounds identified in ME and their abundance were β-sitosterol acetate (16.06%), cholest-5-en-3-yl (9Z)-9-octadecenoate (9.54%), 1-dodecanol (7.57%), (S)-(E)-(−)-4-acetoxy-1-phenyl-2-dodecen-1-one (6.03%), neophytadiene (5.7%), (E)-2-nonadecene (3.9%), hexanol-4-D2 (2.92%), and decane (2.4%). Most compounds have known bioactive functions. In EO, the major compounds were stearyl alcohol (25.38%); cis-9-tetradecenoic acid, isobutyl ester (22.95%); butyl 9-tetradecenoate (10.62%); 11,13-dimethyl-12-tetradecen-1-ol acetate (10.14%); ginsenol (3.48%); and diisooctyl phthalate (2.54%). All compounds are known to be bioactive. The antioxidant activity of ME and EO ranged from 48.3 to 84.2% radical scavenging activity (RSA) and 45.6 to 82% RSA, respectively, with dose dependency. The disc diffusion assay for the antimicrobial activity of ME revealed high inhibition against Acenetobacter baumannii (130.2%), Pseudomonas aeruginosa (100.3%), and Staphylococcus aureus (87.7%). The MIC, MBC/MFC, and MBIC values for ME were 0.5–1.0, 2–4, and 0.5–1.0 mg/mL and for EO were 0.31–0.62, 1.25–2.5, and 0.31–0.62 μL/mL, respectively, indicating inhibition potential as well as inhibition of biofilm formation. The tolerance test values indicated bactericidal activity against most strains and bacteriostatic/fungistatic activity against A. baumannii, E. faecalis, and C. albicans. The antiquorum sensing activity of ME achieved by pyocyanin inhibition assay on P. aeruginosa showed a 51.6% inhibition at 500 μg/mL. These results suggest that ME and EO derived from A. polystachyus leaves are potent, valuable, cost-effective antioxidants and antimicrobials. Both extracts may effectively combat pathogenic and resistant microbes.

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