Optically Active Hybrid Materials Based on Natural Spider Silk

Kiseleva, Aleksandra; Kiselev, Grigorii O.; Kessler, Vadim G.; Seisenbaeva, Gulaim A.; Gets, Dmitry; Rumyantseva, Valeriya; Lyalina, Tatiana; Fakhardo, Anna F.; Krivoshapkin, Pavel V.; Krivoshapkina, Elena F.

doi:10.1021/acsami.9b05131

Cited by 15 publications

(19 citation statements)

References 77 publications

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“…L. fallax was chosen as the producer of the model material in this work because its silk was recently demonstrated to be attractive as a tissue engineering material with beneficial biointernalization characteristics. 82 It is a Bolivian curtain-web spider with an enlarged abdomen that makes up half of the spider’s body. Thus, the amount of silk produced is substantially larger than that of typical orb-web spiders.…”

Section: Resultsmentioning

confidence: 99%

Modulating Surface Properties of the Linothele fallax Spider Web by Solvent Treatment

Kiseleva

Nestor²,

Östman³

et al. 2021

Biomacromolecules

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Linothele fallax (Mello-Leitão) ( L. fallax ) spider web, a potentially attractive tissue engineering material, was investigated using quantitative peak force measurement atomic force microscopy and scanning electron microscopy with energy dispersive spectroscopy both in its natural state and after treatment with solvents of different protein affinities, namely, water, ethanol, and dimethyl sulfoxide (DMSO). Native L. fallax silk threads are densely covered by globular objects, which constitute their inseparable parts. Depending on the solvent, treating L. fallax modifies its appearance. In the case of water and ethanol, the changes are minor. In contrast, DMSO practically removes the globules and fuses the threads into dense bands. Moreover, the solvent treatment influences the chemistry of the threads’ surface, changing their adhesive and, therefore, biocompatibility and cell adhesion properties. On the other hand, the solvent-treated web materials’ contact effect on different types of biological matter differs considerably. Protein-rich matter controls humidity better when wrapped in spider silk treated with more hydrophobic solvents. However, carbohydrate plant materials retain more moisture when wrapped in native spider silk. The extracts produced with the solvents were analyzed using nuclear magnetic resonance (NMR) and liquid chromatography–mass spectrometry techniques, revealing unsaturated fatty acids as representative adsorbed species, which may explain the mild antibacterial effect of the spider silk. The extracted metabolites were similar for the different solvents, meaning that the globules were not “dissolved” but “fused into” the threads themselves, being supposedly rolled-in knots of the protein chain.

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Section: Resultsmentioning

confidence: 99%

Modulating Surface Properties of the Linothele fallax Spider Web by Solvent Treatment

Kiseleva

Nestor²,

Östman³

et al. 2021

Biomacromolecules

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“…It was shown that the upconversion luminescent properties of these nanoparticles could be extended to the macroscale components of the fibrous hybrid while the spidroin backbone remains unaffected. Such spider silk–inorganic nanoparticle-based hybrid materials may have prospective bio-applications in the fields of biosensing and bioimaging in a non-invasive and real-time manner (Kiseleva et al, 2019 ).…”

Section: Fabrication Of Spider Silk-based Hybrid Materialsmentioning

confidence: 99%

Recent Advances in Development of Functional Spider Silk-Based Hybrid Materials

Kiseleva

Krivoshapkin

2020

Front. Chem.

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Silkworm silk is mainly known as a luxurious textile. Spider silk is an alternative to silkworm silk fibers and has much more outstanding properties. Silk diversity ensures variation in its application in nature and industry. This review aims to provide a critical summary of up-to-date fabrication methods of spider silk-based organic-inorganic hybrid materials. This paper focuses on the relationship between the molecular structure of spider silk and its mechanical properties. Such knowledge is essential for understanding the innate properties of spider silk as it provides insight into the sophisticated assembly processes of silk proteins into the distinct polymers as a basis for novel products. In this context, we describe the development of spider silk-based hybrids using both natural and bioengineered spider silk proteins blended with inorganic nanoparticles. The following topics are also covered: the diversity of spider silk, its composition and architecture, the differences between silkworm silk and spider silk, and the biosynthesis of natural silk. Referencing biochemical data and processes, this paper outlines the existing challenges and future outcomes.

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“…Additionally, metal oxides suit well for the preparation of upconverting luminescent materials as matrices for doping owing to their chemical and photochemical stability, high refractive indices and low phonon energies [ 92 , 93 ]. As such, due to the modification of native spider silk fibers with nanoparticles of zirconia (ZrO 2 ) and hafnia (HfO 2 ) doped with rare earth elements, upconversion luminescent properties of the nanoparticles were extended to macro-scale components of the fibrous hybrids while the obtained materials remained biocompatible [ 94 ]. These optically active hybrid materials based on spider silk and inorganic nanoparticles may find applications in the fields of biosensors and bio-imaging in a noninvasive and real-time manner.…”

Section: Modification Of Spider Silk By Inorganic Nanomaterialsmentioning

confidence: 99%

Hybrid Spider Silk with Inorganic Nanomaterials

et al. 2020

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High-performance functional biomaterials are becoming increasingly requested. Numerous natural and artificial polymers have already demonstrated their ability to serve as a basis for bio-composites. Spider silk offers a unique combination of desirable aspects such as biocompatibility, extraordinary mechanical properties, and tunable biodegradability, which are superior to those of most natural and engineered materials. Modifying spider silk with various inorganic nanomaterials with specific properties has led to the development of the hybrid materials with improved functionality. The purpose of using these inorganic nanomaterials is primarily due to their chemical nature, enhanced by large surface areas and quantum size phenomena. Functional properties of nanoparticles can be implemented to macro-scale components to produce silk-based hybrid materials, while spider silk fibers can serve as a matrix to combine the benefits of the functional components. Therefore, it is not surprising that hybrid materials based on spider silk and inorganic nanomaterials are considered extremely promising for potentially attractive applications in various fields, from optics and photonics to tissue regeneration. This review summarizes and discusses evidence of the use of various kinds of inorganic compounds in spider silk modification intended for a multitude of applications. It also provides an insight into approaches for obtaining hybrid silk-based materials via 3D printing.

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Optically Active Hybrid Materials Based on Natural Spider Silk

Cited by 15 publications

References 77 publications

Modulating Surface Properties of the Linothele fallax Spider Web by Solvent Treatment

Modulating Surface Properties of the Linothele fallax Spider Web by Solvent Treatment

Recent Advances in Development of Functional Spider Silk-Based Hybrid Materials

Hybrid Spider Silk with Inorganic Nanomaterials

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