Optical Waveguide Spectroscopy for the Investigation of Protein‐Functionalized Hydrogel Films

Aulasevich, Alena; Roskamp, Robert F.; Jonas, Ulrich; Menges, Bernhard; Dostálek, Jakub; Knoll, Wolfgang

doi:10.1002/marc.200800747

Cited by 41 publications

(51 citation statements)

References 14 publications

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“…A “box” approximation was used in which a potential gradient of the hydrogel density perpendicular to the surface was neglected. 12 A similar approach was used for the measurement of the thickness of a dry hydrogel film d h-dry in contact with air that exhibits a refractive index of n h-dry = 1.48. 14 …”

Section: Methodsmentioning

confidence: 99%

Active Control of SPR by Thermoresponsive Hydrogels for Biosensor Applications

et al. 2013

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The use of thermoresponsive poly(N-isopropylacrylamide)-based hydrogel (pNIPAAm) for rapid tuning of surface plasmon resonance (SPR) is reported. This approach is implemented by using an SPR layer architecture with an embedded indium tin oxide microheater and pNIPAAm film on its top. It takes advantage of rapid thermally induced swelling and collapse of pNIPAAm that is accompanied by large refractive index changes and leads to high thermo-optical coefficient of dn/dT = 2 × 10–2 RIU/K. We show that this material is excellently suited for efficient control of refractive index-sensitive SPR and that it can serve simultaneously as a 3D binding matrix in biosensor applications (if modified with biomolecular recognition elements for a specific capture of target analyte). We demonstrate that this approach enables modulating of the output signal in surface plasmon-enhanced fluorescence spectroscopy biosensors and holds potential for simple time-multiplexing of sensing channels for parallelized readout of fluorescence assays.

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Section: Methodsmentioning

confidence: 99%

Active Control of SPR by Thermoresponsive Hydrogels for Biosensor Applications

et al. 2013

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“…The modification of the layer swelling state due to the variation of the salt concentration or pH were recorded as a measure of the changes in the fluorescence signal from the dye-functionalized dextran. In another example, immunoglobulin G (IgG) antibodies were covalently immobilized in poly( N -isopropylacrylamide) hydrogel layers containing carboxyl groups by reaction via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, N -hydroxysuccinimide, and sodium para- tetrafluorophenol sulfonate to yield responsive sensor matrices for specific immuno-binding [54,55]. In a similar way, IgG antibodies were immobilized in a hydrogel layer of photocrosslinkable carboxymethylated dextran for the detection of the free prostate-specific antigen by employing a biosensor platform based on surface plasmon-enhanced fluorescence spectroscopy with long-range surface plasmons [23].…”

Section: Classification Of Hydrogel Systemsmentioning

confidence: 99%

Thin Hydrogel Films for Optical Biosensor Applications

et al. 2012

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Hydrogel materials consisting of water-swollen polymer networks exhibit a large number of specific properties highly attractive for a variety of optical biosensor applications. This properties profile embraces the aqueous swelling medium as the basis of biocompatibility, non-fouling behavior, and being not cell toxic, while providing high optical quality and transparency. The present review focuses on some of the most interesting aspects of surface-attached hydrogel films as active binding matrices in optical biosensors based on surface plasmon resonance and optical waveguide mode spectroscopy. In particular, the chemical nature, specific properties, and applications of such hydrogel surface architectures for highly sensitive affinity biosensors based on evanescent wave optics are discussed. The specific class of responsive hydrogel systems, which can change their physical state in response to externally applied stimuli, have found large interest as sophisticated materials that provide a complex behavior to hydrogel-based sensing devices.

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“…At a given angle, several resonance frequencies are observed using this set up because of interferences between the guided light wave and the plasmon resonance. Using this technique, both the thickness and the refractive index of the hydrogel film can thus be calculated, contrary to the SPR that only allows for the calculation of the refractive index [98]. …”

Section: Principles Of Readout Platforms Supporting Bioresponsive Hydmentioning

confidence: 99%

Responsive Hydrogels for Label-Free Signal Transduction within Biosensors

Gawel

Barriet

Sletmoen

et al. 2010

Sensors

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Hydrogels have found wide application in biosensors due to their versatile nature. This family of materials is applied in biosensing either to increase the loading capacity compared to two-dimensional surfaces, or to support biospecific hydrogel swelling occurring subsequent to specific recognition of an analyte. This review focuses on various principles underpinning the design of biospecific hydrogels acting through various molecular mechanisms in transducing the recognition event of label-free analytes. Towards this end, we describe several promising hydrogel systems that when combined with the appropriate readout platform and quantitative approach could lead to future real-life applications.

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Optical Waveguide Spectroscopy for the Investigation of Protein‐Functionalized Hydrogel Films

Cited by 41 publications

References 14 publications

Active Control of SPR by Thermoresponsive Hydrogels for Biosensor Applications

Active Control of SPR by Thermoresponsive Hydrogels for Biosensor Applications

Thin Hydrogel Films for Optical Biosensor Applications

Responsive Hydrogels for Label-Free Signal Transduction within Biosensors

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