Optical waveguide lightmode spectroscopy immunosensor for detection of carp vitellogenin

Adányi, Nóra; Majer-Baranyi, Krisztina; Nagy, András; Németh, Gyöngyi; Szendrő, István; Székacs, András

doi:10.1016/j.snb.2012.10.079

Cited by 14 publications

(14 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LOD and LOQ of the proposed biosensor for Vtg (calculated as described in the ''Materials and methods'' section) were 31 and 102.2 ng mL -1 , respectively. The results from our study are in good correlation with those published by both Kim et al (2008) and Adányi et al (2013) showing a similar measurement setup. However, the developed system shows lower sensitivity than monoclonal antibody-based immunosensors for Vtg (Darain et al 2004(Darain et al , 2005.…”

Section: Elisa and Optical Immunosensorsupporting

confidence: 90%

“…The difference in sensitivities between the ELISA and the immunosensor methods is mostly due to the enzyme-linked signal amplification in ELISA. In addition, the lower sensitivity of the direct immunosensor system may be attributed to the greater initial surface mass coverage by immobilized antibodies relative to which the antigen represents a lower mass proportion, as suggested by Adányi et al (2013). In fact, results from different studies demonstrated that moving from a direct to a competitive immunosensor-based method can increase the sensitivity resulting thus more suitable for rapid determination of Vtg in fish samples (Adányi et al 2013).…”

Section: Elisa and Optical Immunosensormentioning

confidence: 99%

“…More recently, Bulukin et al (2007) have proposed an optical immunosensor that, in the order of minutes, is able to detect Vtg detection in carp plasma samples. Similarly, Kim et al (2008) and Adányi et al (2013) have developed an optical waveguide lightmode spectroscopy (OWLS)-based immunosensor for quick and highly sensitive determination of Vtg from different fish tissues. A more simple method to rapidly (i.e.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

et al. 2019

View full text Add to dashboard Cite

Vitellogenin (Vtg) has proven to be a sensitive and simple biomarker in determining sex, sexual maturity, and xenoestrogenic effects in fish. Thus, our investigation has been focused on identification, partial characterization, and quantification of grey mullet (Mugil cephalus) Vtg through the use of a variety of biochemical and immunological analytical techniques. Mullet is considered both a promising aquaculture candidate and an important species for improving sediment quality in polyculture systems. In the first part of this work, grey mullet Vtg was purified from plasma of 17b-estradiol (E2)-induced male fish by a one-step chromatographic protocol, and partially characterized. Specific polyclonal antibodies were then raised against the mullet Vtg, and both an indirect ELISA and an optical immunosensor were set up and validated to quantify plasma Vtg. The indirect ELISA and the optical immunosensor assay developed showed linear measuring in the range 56.8-1047.1 ng mL-1 and 70-739 ng mL-1 Vtg concentrations in standard solutions, respectively. The results obtained suggest that the indirect ELISA allows Vtg detection over a wide dynamic range, thus resulting more suitable for rapid and sensitive sample screening. Therefore, we suggest that the direct immunosensor is a promising tool which needs more investigation to improve the sensitivity.

show abstract

Section: Elisa and Optical Immunosensorsupporting

confidence: 90%

Section: Elisa and Optical Immunosensormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The conjugation reaction was carried out as reported earlier for antibodies specific for Vtg from carp [30]. The resulting antibody-HRP conjugate was purified by gel chromatography using PD-10 columns (Amersham, New Jersey, USA).…”

Section: Elisa Protocolsmentioning

confidence: 99%

“…Monoclonal Vtg antibodies were linked on the sensor surface through chemical cross-linking via a capturing antibody. In a previous publication, our research group demonstrated an immunosensor for Vtg determination in carp (Cyprinus carpio) in both direct and competitive immunoassay format [30]. Kim et al [31,32] reported a very similar direct measurement setup for on-site monitoring of contamination of edible fish with endocrine disruptors.…”

Section: Introductionmentioning

confidence: 99%

Label-free immunosensor for monitoring vitellogenin as a biomarker for exogenous oestrogen compounds in amphibian species

Majer-Baranyi

Adányi

Nagy

et al. 2015

International Journal of Environmental Analytical Chemistry

Self Cite

View full text Add to dashboard Cite

A label-free, optical waveguide lightmode spectroscopy based immunosensor was developed for frog (Bombina orientalis) vitellogenin (Vtg) determination in biological samples as a biomarker for exogenous oestrogen compounds. Antibody against Vtg was produced in rabbits immunised with purified lipovitellin (Lpv), a precursor of Vtg, from the homogenised ovary of oriental fire-bellied toads (B. orientalis). The purified protein and Lpv/Vtg-specific serum were applied in both competitive and direct immunoassay formats using optical waveguide lightmode spectroscopy immunosensor. When measuring Vtg in direct manner, the Lpv antibody (1.76 µg mL −1 ) was immobilised on the sensor surface, and the linear measuring range for Vtg was 0.1-10 µg mL −1 . During the competitive measurement, 100 ng mL −1 Lpv was applied for the immobilisation. The linear measuring range for Vtg was 0.5-50 ng mL −1 . We studied the relative substrate specificity of the antibody, and it was concluded that the method is suitable for the sensitive and selective determination of Vtg levels in toads. Heart, liver and gonad samples from male animals were spiked with Vtg and were analysed using the newly developed method, and female toads and spawn samples were tested and compared to the calibration curve obtained by the spiked samples.

show abstract

Optical Waveguide Biosensors

Duval¹,

Lechuga²

2015

Photonics

View full text Add to dashboard Cite

Optical waveguide lightmode spectroscopy immunosensor for detection of carp vitellogenin

Cited by 14 publications

References 50 publications

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

Label-free immunosensor for monitoring vitellogenin as a biomarker for exogenous oestrogen compounds in amphibian species

Optical Waveguide Biosensors

Contact Info

Product

Resources

About