Optical Spectroscopy for Noninvasive Monitoring of Stem Cell Differentiation

Downes, Andrew; Mouras, Rabah; Elfick, Alistair

doi:10.1155/2010/101864

Cited by 69 publications

(51 citation statements)

References 53 publications

(65 reference statements)

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“…Madre (CM) y la medicina regenerativa, son el control en la diferenciación de CM y asegurar la pureza de las células diferenciadas [1] . Una CM, se define como aquella célula que tiene capacidad de autorenovación y potencialidad de diferenciación, y puede mantenerse en estado indiferenciado por tiempo prolongado.…”

Section: Dos De Los Grandes Retos En La Biología De Las Célulasunclassified

Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

Mata-Miranda

Guerrero-Robles

Rojas-López

et al. 2017

Rev. Mex. Ing. Biomed.

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RESUMENDos de los grandes retos en la biología de las Células Madre (CM) y la Medicina Regenerativa, son el control en la diferenciación de estas células y asegurar la pureza de las células diferenciadas, por lo que es necesario contar con técnicas rápidas, eficientes y precisas para la caracterización de CM y su diferenciación a diferentes linajes celulares. El objetivo de este trabajo fue analizar Células Madre Pluripotentes (CMP) y Células Pancreáticas Diferenciadas (CPD) mediante espectroscopía Infrarroja por Transformada de Fourier (FTIR) y Análisis de Componentes Principales (ACP). Para ello se diferenciaron CMP a CPD, caracterizando el proceso de diferenciación a los días 0, 11, 17 y 21 mediante microscopía óptica y espectroscopia vibracional. Los espectros FTIR se analizaron con el método multivariado de ACP, utilizando su segunda derivada en las regiones de proteínas, carbohidratos y ribosas. Los resultados indican que el ACP permite caracterizar y discriminar CMP y CPD en sus diferentes etapas de diferenciación en las regiones espectrales analizadas. Con lo anterior concluimos que el ACP permite caracterizar química y estructuralmente CMP y diferentes etapas de su diferenciación en una forma rápida, precisa y no invasiva.

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Section: Dos De Los Grandes Retos En La Biología De Las Célulasunclassified

Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

Mata-Miranda

Guerrero-Robles

Rojas-López

et al. 2017

Rev. Mex. Ing. Biomed.

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“…The automation of standard planar culture methods has shown promise, and improved control, but is afflicted by high entry costs and limited scalability. These planar culture system developments have necessitated parallel development of new non-invasive analysis methods, such as image analysis or spectroscopy [32,33]. Finally, systems have been designed for high-density support of adherent cells, often by virtue of diffuse mass exchange via perfused fibres or suspended particles.…”

Section: Alternative Process Platformsmentioning

confidence: 99%

Precision manufacturing for clinical-quality regenerative medicines

Williams

Thomas

Hourd

et al. 2012

Phil. Trans. R. Soc. A.

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Innovations in engineering applied to healthcare make a significant difference to people's lives. Market growth is guaranteed by demographics. Regulation and requirements for good manufacturing practice—extreme levels of repeatability and reliability—demand high-precision process and measurement solutions. Emerging technologies using living biological materials add complexity. This paper presents some results of work demonstrating the precision automated manufacture of living materials, particularly the expansion of populations of human stem cells for therapeutic use as regenerative medicines. The paper also describes quality engineering techniques for precision process design and improvement, and identifies the requirements for manufacturing technology and measurement systems evolution for such therapies.

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“…It has already been used successfully to discriminate, classify and diagnose malignancies and various tumors [166][167][168] without having adverse effect on the cells analyzed. 169 Huser and co-workers 170 have suggested Raman microspectrometry to be a valuable tool in assessing sperm quality for assisted reproduction.…”

Section: Raman Microspectrometrymentioning

confidence: 99%

Sperm preparation: state-of-the-art—physiological aspects and application of advanced sperm preparation methods

Henkel

2011

Asian J Androl

111

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For assisted reproduction technologies (ART), numerous techniques were developed to isolate spermatozoa capable of fertilizing oocytes. While early methodologies only focused on isolating viable, motile spermatozoa, with progress of ART, particularly intracytoplasmic sperm injection (ICSI), it became clear that these parameters are insufficient for the identification of the most suitable spermatozoon for fertilization. Conventional sperm preparation techniques, namely, swim-up, density gradient centrifugation and glass wool filtration, are not efficient enough to produce sperm populations free of DNA damage, because these techniques are not physiological and not modeled on the stringent sperm selection processes taking place in the female genital tract. These processes only allow one male germ cell out of tens of millions to fuse with the oocyte. Sites of sperm selection in the female genital tract are the cervix, uterus, uterotubal junction, oviduct, cumulus oophorus and the zona pellucida. Newer strategies of sperm preparation are founded on: (i) morphological assessment by means of 'motile sperm organelle morphological examination (MSOME)'; (ii) electrical charge; and (iii) molecular binding characteristics of the sperm cell. Whereas separation methods based on electrical charge take advantage of the sperm's adherence to a test tube surface or separate in an electrophoresis, molecular binding techniques use Annexin V or hyaluronic acid (HA) as substrates. Techniques in this category are magnet-activated cell sorting, Annexin V-activated glass wool filtration, flow cytometry and picked spermatozoa for ICSI (PICSI) from HA-coated dishes and HA-containing media. Future developments may include Raman microspectrometry, confocal light absorption and scattering spectroscopic microscopy and polarization microscopy.

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Optical Spectroscopy for Noninvasive Monitoring of Stem Cell Differentiation

Cited by 69 publications

References 53 publications

Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

Precision manufacturing for clinical-quality regenerative medicines

Sperm preparation: state-of-the-art—physiological aspects and application of advanced sperm preparation methods

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