Optical Spectroscopic Method for <i>in vivo</i> Measurement of Cardiac Myoglobin Oxygen Saturation

Schenkman, Kenneth A.; Marble, David R.; Burns, David H.; Feigl, Eric O.

doi:10.1366/0003702991946541

Cited by 42 publications

(50 citation statements)

References 23 publications

(23 reference statements)

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“…In muscle tissue, absorption by static objects (i.e. not blood) was approximated with a 0.6 % myoglobin content (Jansson and Sylvén 1983) (assuming a 76.3 % water content in muscle (Bergstrom, Furst et al 1974)), oxygenized to 90 % (Schenkman, Marble et al 1999a). Myoglobin absorption data was compiled from Bowen (1949), Millar, Moss et al (1996) and Shenkman, Marble et al (1999b).…”

Section: Tissue Modelsmentioning

confidence: 99%

Measurement depth and volume in laser Doppler flowmetry

Fredriksson

Larsson

Strömberg

2009

Microvascular Research

138

128

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Abstract:A new method for estimating the measurement depth and volume in laser Doppler flowmetry (LDF) is presented. The method is based on Monte Carlo simulations of light propagation in tissue. The contribution from each individual Doppler shift is calculated and thereby multiple Doppler shifts are handled correctly. Different LDF setups for both probe based (0.0, 0.25, 0.5, and 1.2 mm source-detector separation) and imaging systems (0.5 and 2.0 mm beam diameter) are considered, at the wavelengths 453 nm, 633 nm, and 780 nm. Non-linear speckle pattern effects are accounted for in the imaging system setups. The effects of tissue optical properties, blood concentration, and blood oxygen saturation are evaluated using both homogeneous tissue models and a layered skin model. The results show that the effect on the measurement depth of changing tissue properties is comparable to the effect of changing the system setup, e.g. sourcedetector separation and wavelength. Skin pigmentation was found to have a negligible effect on the measurement depth. Examples of measurement depths are (values are given for a probe based system with 0.25 mm source-detector separation and an imaging system with a 0.5 mm beam diameter, respectively, both operating at 780 nm): muscle -0.55/0.79 mm; liver -0.40/0.53 mm; gray matter -0.48/0.68 mm; white matter -0.20/0.20 mm; index finger pulp -0.41/0.53 mm; forearm skin -0.53/0.56 mm; heat provoked forearm skin -0.66/0.67 mm.

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Section: Tissue Modelsmentioning

confidence: 99%

Measurement depth and volume in laser Doppler flowmetry

Fredriksson

Larsson

Strömberg

2009

Microvascular Research

138

128

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“…The interpretation and understanding of alveoli-blood or blood-tissue gas exchange, whether being assessed from the observations of arterial and venous O 2 concentrations, 20,51,54 or by intra-tissue chemical signals such as BOLD MRI 46,53 or hemoglobin and myoglobin spectra, 59,60 or by external detection of tracer content such as 15 O-oxygen and 15 O-water through PET studies, 27,51,55,62 depends in general on all the factors involved in the dissociation of oxyhemoglobin. To study the dynamics of O 2 transport and metabolism, it is therefore important to account for the transport and exchange of CO 2 , , and H + as well as O 2 -CO 2 interactions.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Blood–Tissue Exchange of Oxygen, Carbon Dioxide, Bicarbonate, and Hydrogen Ion

Dash

Bassingthwaighte

2006

Ann Biomed Eng

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A detailed nonlinear four-region (red blood cell, plasma, interstitial fluid, and parenchymal cell) axially distributed convection-diffusion-permeation-reaction-binding computational model is developed to study the simultaneous transport and exchange of oxygen (O 2 ) and carbon dioxide (CO 2 ) in the blood-tissue exchange system of the heart. Since the pH variation in blood and tissue influences the transport and exchange of O 2 and CO 2 (Bohr and Haldane effects), and since most CO 2 is transported as (bicarbonate) via the CO 2 hydration (buffering) reaction, the transport and exchange of and H + are also simulated along with that of O 2 and CO 2 . Furthermore, the model accounts for the competitive nonlinear binding of O 2 and CO 2 with the hemoglobin inside the red blood cells (nonlinear O 2 -CO 2 interactions, Bohr and Haldane effects), and myoglobin-facilitated transport of O 2 inside the parenchymal cells. The consumption of O 2 through cytochrome-c oxidase reaction inside the parenchymal cells is based on MichaelisMenten kinetics. The corresponding production of CO 2 is determined by respiratory quotient (RQ), depending on the relative consumption of carbohydrate, protein, and fat. The model gives a physiologically realistic description of O 2 transport and metabolism in the microcirculation of the heart. Furthermore, because model solutions for tracer transients and steady states can be computed highly efficiently, this model may be the preferred vehicle for routine data analysis where repetitive solutions and parameter optimization are required, as is the case in PET imaging for estimating myocardial O 2 consumption.

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“…There could be several reasons for this discrepancy. First, Hb is absent from the sample and Mb may remain partially oxygenated even at very low PO 2 [16] and [31]. Second, the epicardial contamination by the oxygen from the surrounding air is significant for very small hearts [32].…”

Section: -D Imaging Of Tissue Oxygenation In a Rodent Heart With A Mmentioning

confidence: 99%

“…These changes can be detected in the visible and near infrared (NIR) spectral range. Previously, oxygen-dependent changes of Mb in intact myocardium have been detected by visible-NIR point spectroscopy in rodent and swine hearts [12], [13], [14], [15], [16] and [17] and NIR spectroscopic imaging (NIRSI) in swine hearts [18] and [19]. The depth of NIR light penetration into the tissue can reach several mm [19].…”

Section: Introductionmentioning

confidence: 99%

Defects in myoglobin oxygenation in KATP-deficient mouse hearts under normal and stress conditions characterized by near infrared spectroscopy and imaging

Jilkina

Glogowski

Kuzio

et al. 2011

International Journal of Cardiology

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Defects in myoglobin oxygenation in K ATP -deficient mouse hearts under normal and stress conditions characterized by near infrared spectroscopy and imaging Abstract BackgroundDisruption of ATP-sensitive potassium (K ATP ) channel activity results in the development of dilated cardiomyopathy in response to different forms of stress, likely due to the underlying metabolic defects. To further understand the role of Kir6.2-containing channels in the development of cardiac disease, we analysed the left ventricular (LV) wall oxygenation and the physiologic responses induced by acute stress in non-dilated Kir6.2 −/− hearts. MethodsControl (C57BL6) and Kir6.2 −/− mouse hearts were perfused in constant flow Langendorff mode with Krebs-Henseleit buffer. Myocardial oxygenation was evaluated using a newly developed technique, near infrared spectroscopic imaging (NIRSI) of the myoglobin (Mb) oxygen saturation parameter (OSP, ratio of oxy-to total Mb). Results2,4-dinitrophenol (DNP, 50-µM) and isoproterenol (0.1-µM) failed to produce a transient vasodilatory response and caused a significant diastolic pressure increase in Kir6.2 −/− hearts. DNP strongly suppressed contractile function in both groups and induced severe mean OSP decreases in Kir6.2 −/− hearts. Isoproterenol-induced decreases in OSP were similar despite the lack of contractile function stimulation in the Kir6.2 −/− group. The index of OSP spatial heterogeneity (relative dispersion, RD) was lower by 15% in the Kir6.2 −/− group at the baseline conditions. Recovery after stress caused reduction of RD values by 20% (DNP) and 8% (isoproterenol) in controls; however, these values did not change in the Kir6.2 −/− group. Conclusions1) NIRSI can be used to analyse 2-D dynamics of LV oxygenation in rodent models of cardiomyopathy; 2) Kir6.2-containing K ATP channels play an important role in maintaining myocardial oxygenation balance under acute stress conditions and in post-stress recovery.

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Optical Spectroscopic Method for in vivo Measurement of Cardiac Myoglobin Oxygen Saturation

Cited by 42 publications

References 23 publications

Measurement depth and volume in laser Doppler flowmetry

Measurement depth and volume in laser Doppler flowmetry

Simultaneous Blood–Tissue Exchange of Oxygen, Carbon Dioxide, Bicarbonate, and Hydrogen Ion

Defects in myoglobin oxygenation in KATP-deficient mouse hearts under normal and stress conditions characterized by near infrared spectroscopy and imaging

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