Optical skin biopsies by clinical CARS and multiphoton fluorescence/SHG tomography

Abstract

The ultimate challenge for early diagnostics is labelfree high-resolution intratissue imaging without taking physical biopsies. A novel hybrid femtosecond laser tomograph provides in vivo optical biopsies of human skin based on non-linear excitation of autofluorescence and the detection of lipids and water by CARS. Applications include skin cancer detection, biosafety tests of intradermal nanoparticles, and the testing of anti-aging products. μmOverlay (CARS/autofluorescence) of the outermost skin layer (stratum corneum) of a healthy male volunteer

Safety Assessment by Multiphoton Fluorescence/Second Harmonic Generation/Hyper-Rayleigh Scattering Tomography of ZnO Nanoparticles Used in Cosmetic Products

“…As a result, an excitation wavelength of 760 nm was chosen for further investigations. For multiphoton tomography with a lateral and axial resolution of 0.5 and 2 µm, respectively, two-channel detection was used [47]. To distinguish between the spectrally broad two-photon fluorescence (also called autofluorescence) of the skin and the spectrally narrow SHG/HRS signals originating from ZnO, a broadband filter transmitting between 300 and 700 nm and an interference filter (transmission at 380 nm with a spectral width of 14 nm, full width at half maximum), respectively, were used in front of the photo multipliers.…”

Principles of nonlinear microscopy and application possibilities in dermatology

“…ábra) azonosítására, melyek fontos információként szolgálhatnak a bôr barrier funkciójáról, vagy metabolikus állapotáról (36,37). Az elsô in vivo CARS vizsgálatokat König és munkatársai hajtották végre egészséges önkénte-36 2. ábra Ex vivo humán bôr keresztmetszete a különbözô nemlineáris optikai módszerek alkalmazásával seken, valamint pikkelysömörrel érintett betegeken (38). Az idegsejteket burkoló myelinhüvely vizsgálata a ma még gyógyíthatatlan sclerosis multiplex kutatásában jelentôs szerepet játszik.…”

Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser

“…Several efforts have been undertaken to bring multimodal nonlinear imaging into more clinical applications. [7][8][9] Nonetheless, the widespread transfer to a clinical environment for routine biomedical imaging is still at an early stage. To facilitate this transition, two key aspects have to be taken into consideration in the microscopy platforms design: the detection scheme and laser technology.…”