Optical sensing systems for microfluidic devices: A review

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

“…Traditionally used excitation sources for fluorimetric detection are incandescent or arc lamps (halogen or mercury) based on technologies going back over a century, 4 rates, while their main deficiency is the lack of powerful enough emitters in the deep-UV (below 300nm) spectral region [26][27][28].…”

Impact of laser excitation intensity on deep UV fluorescence detection in microchip electrophoresis

“…Accordingly, there is a need for detection schemes in microfluidics capable to probe unlabelled proteins. UV-absorbance detection that is commonly applied for this purpose in classical and HPLC and CE [8] has also been transferred to microfluidics [9], but due to the reduced optical path lengths it is challenging to achieve sufficient sensitivity [10,11].…”

High‐resolution electrophoretic separation and integrated‐waveguide excitation of fluorescent DNA molecules in a lab on a chip

“…Capillary electrophoresis (CE) separation is a powerful method over a broad range of molecular sizes, thanks to flexible MF protocols, exploiting a variety of sieving gel matrices. The inherent advantages of MF CE, high-speed operation and low reagent volumes, can be combined with laser-induced fluorescence (LIF) detection [6][7][8] Furthermore, the spatio-temporal resolution provided by integrated waveguide excitation 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 F o r P e e r R e v i e w 4 can allow for a 20-fold improvement in accuracy if better MF CE separation protocols become available.…”