Optical Sectioning Microscopy and Biological Imaging

Girkin, John M.

doi:10.1002/9781119011804.ch5

Cited by 1 publication

(1 citation statement)

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“…In order to generate the 3D structure of a sample, light microscopes heavily rely on the sequential capture of two-dimensional (2D) images at various focal planes and the subsequent utilisation of computational tools to reconstruct the corresponding 3D structure (Agard, 1984;Girkin, 2015). These techniques have been improving in terms of both axial scanning speed and reconstruction algorithms (O'Holleran and Shaw, 2014;Schniete et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Electrically tunable lenses – eliminating mechanical axial movements during high-speed 3D live imaging

Efstathiou

Draviam

2021

Journal of Cell Science

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The successful investigation of photosensitive and dynamic biological events, such as those in a proliferating tissue or a dividing cell, requires non-intervening high-speed imaging techniques. Electrically tunable lenses (ETLs) are liquid lenses possessing shape-changing capabilities that enable rapid axial shifts of the focal plane, in turn achieving acquisition speeds within the millisecond regime. These human-eye-inspired liquid lenses can enable fast focusing and have been applied in a variety of cell biology studies. Here, we review the history, opportunities and challenges underpinning the use of cost-effective high-speed ETLs. Although other, more expensive solutions for three-dimensional imaging in the millisecond regime are available, ETLs continue to be a powerful, yet inexpensive, contender for live-cell microscopy.

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